Multi-residue fluorescent microspheres immunochromatographic assay for simultaneous determination of macrolides in raw milk

Li, Xiangmei; Shen, Jianzhong; Wang, Qi; Gao, Shaohua; Pei, Xingyao; Jiang, Haiyang; Wen, Kai

doi:10.1007/s00216-015-9078-3

Cited by 52 publications

(17 citation statements)

References 25 publications

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“…Fluorescent microspheres (FMs) have been commonly used in immunoassays because of their stable morphology, high fluorescence efficiency and the ability of surface modification (Eucker et al, 2010;Li et al, 2015;Liang, Zou, Guo, Gui, & Zhu, 2013). In this study, carboxylate-modified FMs were covalently conjugated with antibodies to form FMs-mAb probes.…”

Section: Introductionmentioning

confidence: 99%

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Wang

Zheng

et al. 2017

Food and Agricultural Immunology

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A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L −1 , respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC-MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Wang

Zheng

et al. 2017

Food and Agricultural Immunology

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“…The nitrocellulose membrane coated with the capturing reagents was delivered onto the center of the plastic back plate (polyvinylchloride). Subsequently, the strip was assembled together as described in a previous work (Li and others ).…”

Section: Methodsmentioning

confidence: 99%

Advantage of Eu³⁺‐Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk

Tang

Zhang

Liu

et al. 2017

Journal of Food Science

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Colloidal gold and Eu -doped fluorescent microspheres were applied as labels to develop the immunochromatographic strips for detecting melamine in milk. Under the optimized condition, the visual detection limit of colloidal gold-immunochromatographic test strip (ICTS) was 150 μg/L of melamine in phosphate-buffered saline (PBS), although the visual detection limit of fluorescent nanoparticles (FN)-ICTS was 75 μg/L in PBS. As thermal acceleration test, FN-ICTS could be stored at 37 °C for at least 11 d before sample testing, but the color of the lines on colloidal gold-ICTS faded away after 7-d storage. The visual result of FN-ICTS was more stable than that of colloidal gold-ICTS, and the fluorescence intensity of the line on FN-ICTS could be maintained up to 30 d at 22 °C after sample testing. Once the immunochromatographic strips were used to detect melamine in milk, no negative effect of milk components on the performance of FN-ICTS was identified, whereas the performance of colloidal gold-ICTS was significantly influenced by milk matrix.

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“…QDs, lanthanide chelates (such as europium), and UCNPs were used as fluorescent labels in FIA [80] ( Fig. 4).…”

Section: Fluorescence-linked Immunoassaysmentioning

confidence: 99%

Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

Tao¹,

Peng²,

Liu³

2020

Journal of Food and Drug Analysis

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Veterinary drugs have been widely used in the food industry. Their residues in food products need to be tightly regulated to ensure food safety. In particular, some veterinary drugs are still used illegally, although they have already been banned. Nanomaterials are playing an increasingly important role in analytical chemistry due to their unique properties. Compared to traditional organic dyes and colloidal gold nanoparticle labels, fluorescent nanomaterials appear particularly attractive for the detection of veterinary drug residues. This review summarizes recent advancements of fluorescent biosensors using nanomaterials for the detection of veterinary drug residues in foods. The useful properties of each type of fluorescent nanomaterial are first discussed such as large Stokes shifts, long emission lifetime, and high quantum yields, which are useful for detection in food-related sample matrix. The following target recognition molecules are then reviewed individually including antibodies, aptamers, molecularly imprinted polymers, and metal ion coordination based ligands. Representative bioconjugation and assay methods are discussed for each recognition mechanism. Finally, a few future research directions are outlined in the last section of this review.

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Multi-residue fluorescent microspheres immunochromatographic assay for simultaneous determination of macrolides in raw milk

Cited by 52 publications

References 25 publications

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Advantage of Eu³⁺‐Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk

Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

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Multi-residue fluorescent microspheres immunochromatographic assay for simultaneous determination of macrolides in raw milk

Cited by 52 publications

References 25 publications

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Advantage of Eu3+‐Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk

Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

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Product

Resources

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Advantage of Eu³⁺‐Doped Polystyrene Microspheres Compared with Colloidal Gold Used in Immunochromatographic Assays for the Detection of Melamine in Milk