Multi‐Scale Label‐Free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two‐Photon Microscopy

Chang, Shuaibin; Yang, Jiarui; Novoseltseva, Anna; Abdelhakeem, Ayman; Hyman, Mackenzie; Fu, Xinlei; Li, Chenglin; Chen, Shih‐Chi; Augustinack, Jean C.; Magnain, Caroline; Fischl, Bruce; Mckee, Ann C.; Boas, David A.; Chen, Ichun Anderson; Wang, Hui

doi:10.1002/advs.202303381

Cited by 2 publications

(1 citation statement)

References 76 publications

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“…The samples were submerged in fomblin for the dMRI scan. After dMRI, one coronal slab per brain was embedded in agarose using a customized grater 20 and refractive index matched in either 30% TDE or 50% glycerol for PSOCT imaging. Supplementary Table 1 describes the detailed individual information about the specimens.…”

Section: Samplesmentioning

confidence: 99%

Imaging of developing human brains with ex vivo PSOCT and dMRI

Wang,

Blanke,

Gong

et al. 2024

Preprint

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The human brain undergoes substantial developmental changes in the first five years of life. Particularly in the white matter, myelination of axons occurs near birth and continues at a rapid pace during the first 2 to 3 years. Diffusion MRI (dMRI) has revolutionized our understanding of developmental trajectories in white matter. However, the mm-resolution of in vivo techniques bears significant limitation in revealing the microstructure of the developing brain. Polarization sensitive optical coherence tomography (PSOCT) is a three-dimensional (3D) optical imaging technique that uses polarized light interferometry to target myelinated fiber tracts with micrometer resolution. Previous studies have shown that PSOCT contributes significantly to the elucidation of myelin content and quantification of fiber orientation in adult human brains. In this study, we utilized the PSOCT technique to study developing brains during the first 5 years of life in combination with ex vivo dMRI. The results showed that the optical properties of PSOCT quantitatively reveal the myelination process in young children. The imaging contrast of the optic axis orientation is a sensitive measure of fiber orientations in largely unmyelinated brains as young as 3-months-old. The micrometer resolution of PSOCT provides substantially enriched information about complex fiber networks and complements submillimeter dMRI. This new optical tool offers great potential to reveal the white matter structures in normal neurodevelopment and developmental disorders in unprecedented detail.

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Section: Samplesmentioning

confidence: 99%

Imaging of developing human brains with ex vivo PSOCT and dMRI

Wang,

Blanke,

Gong

et al. 2024

Preprint

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Quantum estimation of the stokes vector rotation for a general polarimetric transformation

Pedram,

Besaga,

Gassab

et al. 2024

New J. Phys.

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Classical polarimetry is a well-established discipline with diverse applications across different branches of science. The burgeoning interest in leveraging quantum resources to achieve highly sensitive measurements has spurred researchers to elucidate the behavior of polarized light within a quantum mechanical framework, thereby fostering the development of a quantum theory of polarimetry. In this work, drawing inspiration from polarimetric investigations in biological tissues, we investigate the precision limits of polarization rotation angle estimation about a known rotation axis, in a quantum polarimetric process, comprising three distinct quantum channels. The rotation angle to be estimated is induced by the retarder channel on the Stokes vector of the probe state. The diattenuator and depolarizer channels, acting on the probe state, can be thought of as effective noise processes. We explore the precision constraints inherent in quantum polarimetry by evaluating the quantum Fisher information for probe states of significance in quantum metrology, namely NOON, Kings of Quantumness, and Coherent states. The effects of the noise channels as well as their ordering is analyzed on the estimation error of the rotation angle to characterize practical and optimal quantum probe states for quantum polarimetry. Furthermore, we propose an experimental framework tailored for NOON state quantum polarimetry, aiming to bridge theoretical insights with empirical validation.

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Multi‐Scale Label‐Free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two‐Photon Microscopy

Cited by 2 publications

References 76 publications

Imaging of developing human brains with ex vivo PSOCT and dMRI

Imaging of developing human brains with ex vivo PSOCT and dMRI

Quantum estimation of the stokes vector rotation for a general polarimetric transformation

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