Multi-stage transcriptome profiling of the neglected food-borne echinostome Artyfechinostomum sufrartyfex reveal potential diagnostic and drug targets

Dahal, Suman; Gour, Pratibha; Raghuvanshi, Saurabh; Prasad, Yugal K.; Saikia, Dipshikha; Ghatani, Sudeep

doi:10.1016/j.actatropica.2022.106564

Cited by 1 publication

(1 citation statement)

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“…The sequences obtained from SignalP 6.0 were evaluated by TargetP v1.1 (http:// www.cbs.dtu.dk/services/TargetP/) (accessed on 1 December 2021) [39] for their subcellular location based on N-terminal pre-sequences (at least the first 130 amino acids of the N-terminus required). The input data was a one-letter amino acid code reset; other symbols got converted to X before processing, and a non-plant option was selected before submitting the input [40][41][42].…”

Section: Target P Servermentioning

confidence: 99%

Genome-Wide Characterization of Effector Protein-Encoding Genes in Sclerospora graminicola and Its Validation in Response to Pearl Millet Downy Mildew Disease Stress

Hadimani

Britto

Udayashankar

et al. 2023

JoF

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Pearl millet [Pennisetum glaucum (L.) R. Br.] is the essential food crop for over ninety million people living in drier parts of India and South Africa. Pearl millet crop production is harshly hindered by numerous biotic stresses. Sclerospora graminicola causes downy mildew disease in pearl millet. Effectors are the proteins secreted by several fungi and bacteria that manipulate the host cell structure and function. This current study aims to identify genes encoding effector proteins from the S. graminicola genome and validate them through molecular techniques. In silico analyses were employed for candidate effector prediction. A total of 845 secretory transmembrane proteins were predicted, out of which 35 proteins carrying LxLFLAK (Leucine–any amino acid–Phenylalanine–Leucine–Alanine–Lysine) motif were crinkler, 52 RxLR (Arginine, any amino acid, Leucine, Arginine), and 17 RxLR-dEER putative effector proteins. Gene validation analysis of 17 RxLR-dEER effector protein-producing genes was carried out, of which 5genes were amplified on the gel. These novel gene sequences were submitted to NCBI. This study is the first report on the identification and characterization of effector genes in Sclerospora graminicola. This dataset will aid in the integration of effector classes that act independently, paving the way to investigate how pearl millet responds to effector protein interactions. These results will assist in identifying functional effector proteins involving the omic approach using newer bioinformatics tools to protect pearl millet plants against downy mildew stress. Considered together, the identified effector protein-encoding functional genes can be utilized in screening oomycetes downy mildew diseases in other crops across the globe.

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