Multi-strain probiotic ameliorated toxic effects of phthalates and bisphenol A mixture in Wistar rats

Context Di-2-ethylhexyl phthalate (DEHP), a known persistent organic pollutant, can increase the sperm DNA fragmentation index (DFI). Objective To investigate the mechanism underlying the repair of DEHP-induced sperm DNA damage in mice by Wuwei Fuzheng Yijing (WFY) formula. Materials and methods The potential targets of WFY and sperm DNA fragment (SDF) were obtained from the TCMSP, BATMAN-TCM, OMIM and GeneCards. The protein–protein interaction (PPI) network, GO and KEGG pathway analyses of WFY-SDF were constructed. An animal model of DEHP-induced sperm DNA damage was replicated by gavage of SPF ICR (CD1) mice DEHP at 1 g/kg/d and treated with WFY at 8.92, 17.84 and 35.67 g/kg, respectively, for 60 d. Sperm DFI of each group was detected and compared. The target genes of WFY identified by transcriptomic and proteomic analyses were validated by qRT-PCR and Western blotting. Results Network pharmacology pathway analysis indicated that PI3K/Akt was the potential target of WFY on SDF. The DFI of the DEHP group (25.48%) was significantly higher than that of the control group (4.02%). The high-dose WFY group (19.05%) exhibited the most significant repairing effect. The related pathways were PI3K/Akt and metabolic. Aass, Aldh1a7, GSTA3, betaine homocysteine S -methyltransferase (Bhmt), Mug2 and Svs1 were screened and Bhmt was validated. Discussion and conclusions WFY can repair sperm DNA damage caused by DEHP, and the mechanism may be related to PI3K/Akt and metabolic pathways, and Bhmt. This provides a new direction for using traditional Chinese medicine to prevent and repair reproductive system injury caused by pollutants.

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“…These pollutants can cause damage to various organs of the body (Baralić et al. 2020 , 2021 ; Park et al. 2020).…”

Section: Discussionmentioning

confidence: 99%

Repair mechanism of Wuwei Fuzheng Yijing formula in di-2-ethylhexyl phthalate-induced sperm DNA fragmentation in mice

Zhang

Wang

et al. 2022

Pharmaceutical Biology

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“…In another study, one by one and mix treatment with probiotics such as Saccharomyces boulardii, Lactobacillus rhamnosus, Lactobacillus planarum LP 6595, Lactobacillus planarum, attenuated the toxic effects of a DEHP, Di-n-butyl phthalate (DBP) and Bisphenol A mixture. Probiotic usage improved serum glucose levels and organ size changes, and reduced inflammation processes in different organs such as the liver [38]. Nevertheless, studies on phthalate types and their effects on gut microbiota, as well as on the determination of changes in different types of phthalate toxic action mechanisms in probiotic supplementation, are very scarce in the literature.…”

Section: Phthalatesmentioning

confidence: 99%

Can probiotics win the battle against environmental endocrine disruptors?

Sevim¹,

Kara²

2021

Arhiv za farmaciju

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Compounds that have negative effects on the endocrine system are called endocrine disrupting compounds (EDCs). There are several different types of compounds, with several different usage areas in the environment, which can be classified as EDCs. These chemicals have a wide range of negative health effects in organisms, depending on their target hormone system. EDCs are among the most popular topics of scientific research, as they are widely used and organisms are frequently exposed to these chemicals. There are various exposure routes for EDCs, such as oral, inhalation and dermal exposure. Parabens, phenolic compounds, phthalates, and pesticides are the most common EDCs. Nowadays, intestinal microorganism distribution, probiotics, and food supplements that regulate these microorganisms and their protective effects against various harmful chemicals attract attention. For this reason, many studies have been carried out in this field and certain diet schemes have been created according to the results of these studies. In fact, probiotics are preferred in order to reduce and eliminate the negative effects of harmful chemicals and to ensure that the organism reacts strongly in these conditions. In this review, we will focus on EDCs, their health effects and positive effects of probiotics on EDCs exposure conditions.

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“…Recently, the present micropillar/microwell platform can be applicable as a cancer spheroid array chip for selecting effective drugs [18]. Some materials, such as luteolin-supplemented diets and multi-strain probiotics, showed to ameliorate toxic effects of BPA in vitro and in vivo studies via anti-oxidative stress and anti-inflammation mechanisms [19,20]. In the present study, we evaluated BPA detoxification with the materials that could stimulate metabolic enzymes.…”

Section: Changes In Bpa Toxicity In the Presence Of Korean Pear Extract Or Arbutinmentioning

confidence: 99%

Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Pyrus pyrifolia)

Lee

Koh

et al. 2020

Micromachines

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A micropillar/microwell chip platform with 3D cultured liver cells has been used for HTP screening of hepatotoxicity of bisphenol A (BPA), an endocrine-disrupting chemical. We previously found the hepatotoxicity of BPA is alleviated by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase 2 (ALDH2). In this study, we have tested potential BPA detoxification with Korean pear (Pyrus pyrifolia) extract, stimulators of ADH and ALDH, as well as arbutin, a reference compound in the pears, on the micropillar/microwell chip platform with human liver cells. Surprisingly, the toxicity of BPA was reduced in the presence of Korean pear extract, indicated by significantly increased IC50 values. The IC50 value of BPA with Korean pear extract tested against HepG2 cells was shifted from 151 to 451 μM, whereas those tested against Hep3B cells was shifted from 110 to 204 μM. Among the tested various concentrations, 1.25, 2.5, and 5 mg/mL of the extract significantly reduced BPA toxicity (Ps < 0.05). However, there was no such detoxification effects with arbutin. This result was supported by changes in protein levels of ADH in the liver cells.

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Multi-strain probiotic ameliorated toxic effects of phthalates and bisphenol A mixture in Wistar rats

Cited by 35 publications

References 76 publications

Repair mechanism of Wuwei Fuzheng Yijing formula in di-2-ethylhexyl phthalate-induced sperm DNA fragmentation in mice

Repair mechanism of Wuwei Fuzheng Yijing formula in di-2-ethylhexyl phthalate-induced sperm DNA fragmentation in mice

Can probiotics win the battle against environmental endocrine disruptors?

Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Pyrus pyrifolia)

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