Multi-target polydiacetylene liposome-based biosensor for improved exosome detection

Kim, Changheon; Han, Bo Hoon; Kim, Dongwoo; Lee, Gyubok; Hong, Changgi; Kang, Ji Yoon; Lee, Kangwon

doi:10.1016/j.snb.2021.131286

Cited by 11 publications

(6 citation statements)

References 40 publications

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“…Exosomes are a type of membrane vesicles derived from multiple cellular sources and are regarded as multiple therapeutic targets (32). ADSCs‐derived extracellular vesicles are involved in a broad range of biological effects, such as UV protection, fibroblast proliferation, collagen synthesis, DNA repair and cell aging (33).…”

Section: Discussionmentioning

confidence: 99%

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Zhang

Yuan

Huang

et al. 2023

Photochem & Photobiology

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UVB‐induced photoaging is characterized by wrinkle formation, slackness and senile plaques, affecting the health and beauty of human being. Our previous study revealed that exosomes derived from adipose‐derived stem cells (ADSCs) could efficiently alleviate UVB‐induced photodamage. However, the functional ingredients in exosomes were undefined. LncRNA H19, one of the well‐researched lncRNAs in exosomes, exhibits multiple physiological effects. This study aims to demonstrate the photo‐protective role of lncRNA H19 on skin photoaging in UVB‐irradiated human skin fibroblasts cells (HSFs) and Kunming mice. LncRNA H19‐overexpressing exosomes (H19‐Exo) were isolated from the supernatant of ADSCs infected with lncRNA H19‐loaded lentivirus. The results showed that H19‐Exo significantly inhibited MMPs production, DNA damage and ROS generation while enhancing procollagen type I synthesis in UVB‐irradiated HSFs. Meanwhile, H19‐Exo markedly reversed epidermal thickening and collagen degradation in UVB‐irradiated mice. Furthermore, luciferase reporter assays indicated that lncRNA H19 acted as a sponge for miR‐138 expression, and SIRT1 was targeted by miR‐138. Evidence from both in vitro and in vivo studies also revealed that H19‐Exo could enhance SIRT1 expression by knocking down miR‐138. In conclusion, lncRNA H19 served as a therapeutic candidate in treating UVB‐induced skin photoaging by upregulation of SIRT1 via miR‐138.

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Section: Discussionmentioning

confidence: 99%

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Zhang

Yuan

Huang

et al. 2023

Photochem & Photobiology

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“…112 In a recent paper, the authors have developed a liposome-based biosensor to detect biomarkers for potential therapeutic applications. 113 They used polydiacetylene-based liposomes, which have unique optical properties and can detect biomarkers by an easy-to-visualize colorimetric approach. Similar biobased nanoparticles can be used for biosensing-based theranostic applications.…”

Section: Liver and Kidney Diseasesmentioning

confidence: 99%

“…In the case of exosome-based sensors, the challenge is associated with the sensitive isolation and screening methods, resulting in low density, small size, and scarcity of exosomes . In a recent paper, the authors have developed a liposome-based biosensor to detect biomarkers for potential therapeutic applications . They used polydiacetylene-based liposomes, which have unique optical properties and can detect biomarkers by an easy-to-visualize colorimetric approach.…”

Section: Potential Applicationsmentioning

confidence: 99%

Tailoring the Inherent Properties of Biobased Nanoparticles for Nanomedicine

Zahid

Chakraborty

Luo

et al. 2023

ACS Biomater. Sci. Eng.

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Biobased nanoparticles are at the leading edge of the rapidly developing field of nanomedicine and biotherapeutics. Their unique size, shape, and biophysical properties make them attractive tools for biomedical research, including vaccination, targeted drug delivery, and immune therapy. These nanoparticles are engineered to present native cell receptors and proteins on their surfaces, providing a biomimicking camouflage for therapeutic cargo to evade rapid degradation, immune rejection, inflammation, and clearance. Despite showing promising clinical relevance, commercial implementation of these biobased nanoparticles is yet to be fully realized. In this perspective, we discuss advanced biobased nanoparticle designs used in medical applications, such as cell membrane nanoparticles, exosomes, and synthetic lipid-derived nanoparticles, and highlight their benefits and potential challenges. Moreover, we critically assess the future of preparing such particles using artificial intelligence and machine learning. These advanced computational tools will be able to predict the functional composition and behavior of the proteins and cell receptors present on the nanoparticle surfaces. With more advancement in designing new biobased nanoparticles, this field of research could play a key role in dictating the future rational design of drug transporters, thereby ultimately improving overall therapeutic outcomes.

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“…[33] Furthermore, a gradual change depending on the intensity of the stimulus can be observed with the absorbance change. Colorimetric sensors using the special optical characteristics of PDA have been developed in various fields and successfully applied to the visual detection of exosomes, [34] viruses, [35,36] chemicals, [37,38] arteriosclerosis, [39] and platelets. [40] PDA-based colorimetric sen-sors showed potential as a low-cost, high-efficiency sensor that can detect targets through colorimetric change without special equipment.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Detection of HER2‐Overexpressing‐Cancer‐Derived Exosomes in Mouse Urine Using Magnetic‐Polydiacetylene Nanoparticles

Kim,

Mun,

Lim

et al. 2023

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Breast cancer (BC) is a major global health problem, with ≈20–25% of patients overexpressing human epidermal growth factor receptor 2 (HER2), an aggressive marker, yet access to early detection and treatment varies across countries. A low‐cost, equipment‐free, and easy‐to‐use polydiacetylene (PDA)‐based colorimetric sensor is developed for HER2‐overexpressing cancer detection, designed for use in low‐ and middle‐income countries (LMICs). PDA nanoparticles are first prepared through thin‐film hydration. Subsequently, hydrophilic magnetic nanoparticles and HER2 antibodies are sequentially conjugated to them. The synthesized HER2‐MPDA can be concentrated and separated by a magnetic field while inheriting the optical characteristics of PDA. The specific binding of HER2 antibody in HER2‐MPDA to HER2 receptor in HER2‐overexpressing exosomes causes a blue‐to‐red color change by altering the molecular structure of the PDA backbone. This colorimetric sensor can simultaneously separate and detect HER2‐overexpressing exosomes. HER2‐MPDA can detect HER2‐overexpressing exosomes in the culture medium of HER2‐overexpressing BC cells and in mouse urine samples from a HER2‐overexpressing BC mouse model. It can selectively isolate and detect only HER2‐overexpressing exosomes through magnetic separation, and its detection limit is found to be 8.5 × 108 particles mL−1. This colorimetric sensor can be used for point‐of‐care diagnosis of HER2‐overexpressing BC in LMICs.

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Multi-target polydiacetylene liposome-based biosensor for improved exosome detection

Cited by 11 publications

References 40 publications

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Long Non‐coding RNA H19‐Overexpressing Exosomes Ameliorate UVB‐Induced Photoaging by Upregulating SIRT1Via Sponging miR‐138

Tailoring the Inherent Properties of Biobased Nanoparticles for Nanomedicine

Colorimetric Detection of HER2‐Overexpressing‐Cancer‐Derived Exosomes in Mouse Urine Using Magnetic‐Polydiacetylene Nanoparticles

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