Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of
            <i>Mycobacterium tuberculosis</i>

Bémer, Pascale; Palicova, Frantiska; Rüsch-Gerdes, Sabine; Drugeon, H; Pfyffer, Gaby E.

doi:10.1128/jcm.40.1.150-154.2002

Cited by 158 publications

(115 citation statements)

References 11 publications

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“…Re-testing of strains with discordant results was performed both on LJ medium (as before), and the Bactec MGIT 960 system (5.0 mg/ml for EMB) as described previously. 4 For MIC determination, suspensions were made from fresh cultures grown on LJ. The suspensions were adjusted to the optical density of McFarland 1.…”

Section: Strain Cultivation and Drug Susceptibility Testingmentioning

confidence: 99%

Tuberculosis ethambutol resistance: Concordance between phenotypic and genotypic test results

Plinke

Cox

Kalon³

et al. 2009

Tuberculosis

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s u m m a r y embB306 mutations are potential markers for detecting ethambutol resistance in clinical Mycobacterium tuberculosis isolates. However, more recently, embB306 mutations have been found in ethambutol susceptible isolates and an association with broad drug resistance rather than ethambutol resistance has been reported.To further investigate this question, we analyzed the association between embB306 mutations and phenotypic ethambutol resistance among 197 isolates from a drug resistance survey performed in Karakalpakstan, Uzbekistan.39 strains had an embB306 mutation, out of which seven were ethambutol susceptible, thus, displaying discrepant test results. After re-analysis, the seven isolates were tested ethambutol resistant. All of these strains had an increased ethambutol MIC, however, three strains showed no or weak growth on the critical concentration of 2 mg/ml on Lö wenstein-Jensen. In three strains we confirmed the presence of heteroresistant mixed populations which might influence conventional ethambutol testing. Final concordance between molecular and phenotypic EMB testing was high with a sensitivity of 78% and a specificity of 100%.Our results confirm that embB306 mutations are useful markers for predicting ethambutol resistance. Discrepancies between molecular and phenotypic ethambutol resistance test results are most likely caused by problems with conventional susceptibility testing.

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Section: Strain Cultivation and Drug Susceptibility Testingmentioning

confidence: 99%

Tuberculosis ethambutol resistance: Concordance between phenotypic and genotypic test results

Plinke

Cox

Kalon³

et al. 2009

Tuberculosis

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“…[95][96][97][98] Reductions in turnaround times from 21 days for LJ-based tests to 6-12 days for automated liquid culture systems for susceptibility testing have been reported. 98,99 The liquid culture methods are expensive, however, and require elaborate technology. 100 In addition, skilled and experienced staff are crucial as contamination rates have been high when inexperienced and untrained staff have used these systems.…”

Section: Rapid Liquid Culture Systemsmentioning

confidence: 99%

A systematic review of rapid diagnostic tests for the detection of tuberculosis infection

Dinnes¹,

Deeks²,

Kunst³

et al. 2007

Health Technol Assess

538

361

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“…The growth of bacteria enhances the fluorescence (emission of light). The growth of bacteria is visually observed in the presence of nonhomogeneous light turbidity or appearance of small granular in the medium 38,40 .…”

Section: Modern Methods For Drug-resistant Tuberculosismentioning

confidence: 99%

Tuberculosis: A Literature Review

Shivanand¹,

Mukhopadhayay²

2017

Int. Res. J. Pharm.

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Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of Mycobacterium tuberculosis

Cited by 158 publications

References 11 publications

Tuberculosis ethambutol resistance: Concordance between phenotypic and genotypic test results

Tuberculosis ethambutol resistance: Concordance between phenotypic and genotypic test results

A systematic review of rapid diagnostic tests for the detection of tuberculosis infection

Tuberculosis: A Literature Review

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