Multidimensional chromatography in environmental analysis: Comprehensive two-dimensional liquid versus gas chromatography

Duarte, Regina M.B.O.; Brandão, Pedro F.; Duarte, Armando C.

doi:10.1016/j.chroma.2023.464288

Cited by 4 publications

(1 citation statement)

References 53 publications

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“…Therefore, enhancing separation capabilities and reducing the coelution fraction are essential for improving the sensitivity and reproducibility of LC-MS. Recently, two-dimensional (2D) LC-HRMS has emerged as a powerful tool for profiling complex chemical systems, such as natural products, biosamples, environmental contaminants and food additives [ 5 , 6 , 7 ]. 2D LC significantly increases peak capacities to several thousand or even over 10,000 by connecting two columns with different separation mechanisms in series, such as the combination of normal-phase (NP) and reversed-phase (RP), ion exchange (IE) and RP, as well as HILIC and RP [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Characterization of Triterpene Saponins in Rhizoma Panacis Japonici by Offline Two-Dimensional Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry

Yasen,

Li,

Wang

et al. 2024

Molecules

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Rhizoma Panacis Japonici (RPJ) is an ancient herbal medicine from China that has long been employed for its medicinal benefits in relieving arthritis physical debility and diverse afflictions. The primary bioactive constituents found in RPJ are triterpene saponins, which exhibit numerous pharmacological actions, including anti-inflammatory, antioxidant, and immunomodulating effects. The present study established a straightforward and effective approach for characterizing triterpene saponins in RPJ. An offline HILIC × RP LC/QTOF-MS method was developed, along with a self-constructed in-house database containing 612 saponins reported in the Panax genus and 228 predicted metabolites. The approach achieved good chromatographic performance in isolating triterpene saponins of RPJ, with the HILIC column as the first dimension (1D) and the BEH C18 column as the second dimension (2D). The developed two-dimensional liquid chromatography system exhibited an orthogonality of 0.61 and a peak capacity of 1249. Detection was performed using a QTOF mass spectrometer in a data-independent manner (MSE) in a negative ion mode. Using the in-house database, the collected MS data were processed by an automatic workflow on UNIFI 1.8.2 software, which included data correction, matching of precursor and product ions, and peak annotation. In this study, 307 saponins were characterized from RPJ and 76 saponins were identified for the first time in Panax japonicus. This research not only enhances our understanding of the chemical characteristics of RPJ but also offers a simple and efficient method for analyzing the complex composition of herbal medicine.

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