Multidisciplinary approach for surveillance and risk identification of yellow fever and other arboviruses in Colombia

Mantilla, Juan S.; Sarmiento, Diana; Manzano, Jaime; Calderón-Peláez, María-Angélica; Velandia-Romero, Myriam L.; Buitrago, Luz Stella; Castellanos, Jaime E.; Olano, Víctor Alberto

doi:10.1016/j.onehlt.2022.100438

Cited by 5 publications

(4 citation statements)

References 38 publications

(44 reference statements)

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“…DENV detection was demonstrated in all three studies when testing larvae derived from laboratory-infected mosquitoes for virus detection. DENV also could be detected from filed-caught larvae in most studies (22/26) using various methods [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Twenty-six studies used pooled immature specimens when detecting dengue virus.…”

Section: Resultsmentioning

confidence: 99%

Potential Way to Develop Dengue Virus Detection in Aedes Larvae as an Alternative for Dengue Active Surveillance: A Literature Review

Rachmawati,

Ekawardhani,

Fauziah

et al. 2024

TropicalMed

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The burden of dengue has emerged as a serious public health issue due to its impact on morbidity, mortality, and economic burden. Existing surveillance systems are inadequate to provide the necessary data for the prompt and efficient control of dengue. Passive surveillance of dengue cases may lead to underreporting and delayed mitigation responses. Improved dengue control program requires sensitive and proactive methods for early detection of dengue. We collected and reviewed existing research articles worldwide on detecting dengue virus in Aedes species larvae. Searches were conducted in PUBMED and Google Scholar, including all the studies published in English and Bahasa Indonesia. Twenty-nine studies were included in this review in terms of assay used, positivity rate, and dengue serotype detected. The presence of dengue virus in immature mosquitoes was mostly detected using reverse transcription PCR (RT-PCR) in pooled larvae. In one study, dengue virus was detected in larvae from laboratory-infected mosquitoes using enzyme-linked immunosorbent assay (ELISA). The positivity rate of dengue virus detection ranged from 0 to 50% in field-caught larvae. Although various methods can detect the dengue virus, further research encourages the use of low-cost and less laborious methods for active surveillance of dengue in larvae.

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Section: Resultsmentioning

confidence: 99%

Potential Way to Develop Dengue Virus Detection in Aedes Larvae as an Alternative for Dengue Active Surveillance: A Literature Review

Rachmawati,

Ekawardhani,

Fauziah

et al. 2024

TropicalMed

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“…The specificity of ISV to invertebrate hosts is related to genetic, structural, immunological, and microclimatic conditions (Elrefaey et al, 2020 ). In addition, multiple infections with arboviruses such as DENV, ZIKV, YFV, and CHIKV have been found in field-collected mosquitoes, with evidence of vertical transmission (Velandia-Romero et al, 2017 ; Alencar et al, 2021 ; Mantilla-Granados et al, 2022 ). Thus, arboviruses are a part of the normal microbiota of these insect vectors, making them reservoirs.…”

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

“…Arbovirus co-infections, even from different arbovirus families, such as Flaviviridae (DENV, ZIKV, and YFV) and Togaviridae (CHIKV), are possible in wild Aedes spp. mosquitoes (Mantilla-Granados et al, 2022 ). Arboviruses can exhibit different strategies to evade the immunological response of mosquitoes.…”

Section: Mosquitoes and Arbovirusmentioning

confidence: 99%

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

Mantilla-Granados,

Castellanos,

Velandia-Romero

2024

Front. Microbiol.

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Arboviral infections transmitted by Aedes spp. mosquitoes are a major threat to human health, particularly in tropical regions but are expanding to temperate regions. The ability of Aedes aegypti and Aedes albopictus to transmit multiple arboviruses involves a complex relationship between mosquitoes and the virus, with recent discoveries shedding light on it. Furthermore, this relationship is not solely between mosquitoes and arboviruses, but also involves the mosquito microbiome. Here, we aimed to construct a comprehensive review of the latest information about the arbovirus infection process in A. aegypti and A. albopictus, the source of mosquito microbiota, and its interaction with the arbovirus infection process, in terms of its implications for vectorial competence. First, we summarized studies showing a new mechanism for arbovirus infection at the cellular level, recently described innate immunological pathways, and the mechanism of adaptive response in mosquitoes. Second, we addressed the general sources of the Aedes mosquito microbiota (bacteria, fungi, and viruses) during their life cycle, and the geographical reports of the most common microbiota in adults mosquitoes. How the microbiota interacts directly or indirectly with arbovirus transmission, thereby modifying vectorial competence. We highlight the complexity of this tripartite relationship, influenced by intrinsic and extrinsic conditions at different geographical scales, with many gaps to fill and promising directions for developing strategies to control arbovirus transmission and to gain a better understanding of vectorial competence. The interactions between mosquitoes, arboviruses and their associated microbiota are yet to be investigated in depth.

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“…Colombia, a world-renowned megadiverse nation, has a rich variety of mosquito species 33 .This diversity, coupled with the diversity of ecosystems, creates favorable conditions in approximately 85% of the country for the migration, adaptation, and proliferation of mosquitoes, which contributes to the increased prevalence of mosquito-borne diseases in the country 34 – 36 . The high richness of mosquitoes in different regions of the country is probably associated with landscape modifications, deforestation and changes in temperature 8 , 34 favoring the proximity of wildlife vectors to humans, which increases the risk of transmission of known and emerging zoonotic arboviruses 37 , 38 . In the country, entomovirological surveillance efforts have focused mainly on viral species of public health importance 36 , 38 , especially in urban centers, while exploration of other viral species remains limited.…”

Section: Introductionmentioning

confidence: 99%

Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco: insights from a preliminary metagenomic study

Gómez,

Martínez,

Páez-Triana

et al. 2023

Sci Rep

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Mosquitoes (Diptera: Culicidae) are primary vectors of arthropod-borne viruses (arboviruses) that pose significant public health threats. Recent advances in sequencing technology emphasize the importance of understanding the arboviruses and insect-specific viruses (ISVs) hosted by mosquitoes, collectively called the “virome”. Colombia, a tropical country with favorable conditions for the development and adaptation of multiple species of Culicidae, offers a favorable scenario for the transmission of epidemiologically important arboviruses. However, entomovirological surveillance studies are scarce in rural areas of the country, where humans, mosquitoes, and animals (both domestic and wild) coexist, leading to a higher risk of transmission of zoonotic diseases to humans. Thus, our study aimed to perform a preliminary metagenomic analysis of the mosquitoes of special relevance to public health belonging to the genera Ochlerotatus, Culex, Limatus, Mansonia, Psorophora, and Sabethes, within a rural savanna ecosystem in the Colombian Orinoco. We employed third-generation sequencing technology (Oxford Nanopore Technologies; ONT) to describe the virome of mosquitoes samples. Our results revealed that the virome was primarily shaped by insect-specific viruses (ISVs), with the Iflaviridae family being the most prevalent across all mosquito samples. Furthermore, we identified a group of ISVs that were common in all mosquito species tested, displaying the highest relative abundance concerning other groups of viruses. Notably, Hanko iflavirus-1 was especially prevalent in Culex eknomios (88.4%) and Ochlerotatus serratus (88.0%). Additionally, other ISVs, such as Guadalupe mosquito virus (GMV), Hubei mosquito virus1 (HMV1), Uxmal virus, Tanay virus, Cordoba virus, and Castlerea virus (all belonging to the Negevirus genus), were found as common viral species among the mosquitoes, although in lower proportions. These initial findings contribute to our understanding of ISVs within mosquito vectors of the Culicidae family in the Eastern Plains of Colombia. We recommend that future research explore deeper into ISV species shared among diverse vector species, and their potential interactions with arboviruses. In addition, we also showed the need for a thorough exploration of the influence of local rural habitat conditions on the shape of the virome in mosquito vectors.

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Multidisciplinary approach for surveillance and risk identification of yellow fever and other arboviruses in Colombia

Cited by 5 publications

References 38 publications

Potential Way to Develop Dengue Virus Detection in Aedes Larvae as an Alternative for Dengue Active Surveillance: A Literature Review

Potential Way to Develop Dengue Virus Detection in Aedes Larvae as an Alternative for Dengue Active Surveillance: A Literature Review

A tangled threesome: understanding arbovirus infection in Aedes spp. and the effect of the mosquito microbiota

Characterizing viral species in mosquitoes (Culicidae) in the Colombian Orinoco: insights from a preliminary metagenomic study

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