Multidrug release based on microneedle arrays filled with pH-responsive PLGA hollow microspheres

Ke, Cherng-Jyh; Lin, Yu-Min; Hu, Yichen; Chiang, Wei-Lun; Chen, Ko-Jie; Yang, Wencheng; Liu, Hao-Li; Fu, Chien-Chung; Sung, Hsing‐Wen

doi:10.1016/j.biomaterials.2012.03.056

Cited by 128 publications

(76 citation statements)

References 32 publications

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“…We chose these needle sizes because the usefulness of these microneedles are commonly investigated. 17,19,37) Microneedles having a needle length of 500 µm were likely to be more effective than 300-µm microneedles in terms of activation of MHC class II on LCs (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Takeuchi

Nomoto

Watanabe

et al. 2016

Biological & Pharmaceutical Bulletin

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An adequate immune response to percutaneous vaccine application is generated by delivery of sufficient amounts of antigen to skin and by administration of toxin adjuvants or invasive skin abrasion that leads to an adjuvant effect. Microneedles penetrate the stratum corneum, the outermost layer of the skin, and enable direct delivery of vaccines from the surface into the skin, where immunocompetent dendritic cells are densely distributed. However, whether the application of microneedles to the skin activates antigen-presenting cells (APCs) has not been demonstrated. Here we aimed to demonstrate that microneedles may act as a potent physical adjuvant for successful transcutaneous immunization (TCI). We prepared samples of isolated epidermal and dermal cells and analyzed the expression of major histocompatibility complex (MHC) class II and costimulatory molecules on Langerhans or dermal dendritic cells in the prepared samples using flow cytometry. The expression of MHC class II and costimulatory molecules demonstrated an upward trend in APCs in the skin after the application of 500-and 300-µm microneedles. In addition, in the epidermal cells, application of microneedles induced more effective activation of Langerhans cells than did an invasive tapestripping (positive control). In conclusion, the use of microneedles is likely to have a positive effect not only as an antigen delivery system but also as a physical technique inducing an adjuvant-like effect for TCI.

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Section: Discussionmentioning

confidence: 99%

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Takeuchi

Nomoto

Watanabe

et al. 2016

Biological & Pharmaceutical Bulletin

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“…The obtained IONPs were then thoroughly rinsed by acetone to remove the free oleic acid. HMs of PLGA (10 mg/mL) that contained IONPs (6 mg/mL) and DOX (1 mg/mL) were prepared using a water-in-oil-in-water (W/O/W) double-emulsion, solvent-diffusion-evaporation technique, which has been described previously [19,20]. The T g of PLGA HMs was evaluated using a differential scanning calorimeter (DSC, PerkinElmer, Norwalk, CT, USA); the heating rate was controlled at 10 C/min, and each sample was heated and cooled for five cycles between 20 and 60 C.…”

Section: Preparation and Characterization Of Lipos-hmsmentioning

confidence: 99%

Localized sequence-specific release of a chemopreventive agent and an anticancer drug in a time-controllable manner to enhance therapeutic efficacy

et al. 2016

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“…The excipient of maltose in SH-DM dissolved quickly after tissue fluid absorption and drug started to release. The degradation of PLGA was slow 61 , which acted as the skeleton of DM and postponed microchannels closing. This design further enhanced the drug permeability into skin.…”

Section: Pharmacokineticsmentioning

confidence: 99%

Sinomenine hydrochloride-loaded dissolving microneedles enhanced its absorption in rabbits

Chen

Shen

et al. 2015

Pharmaceutical Development and Technology

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Sinomenine hydrochloride-loaded dissolving microneedles (SH-DM) were fabricated by maltose and poly-lactic-co-glycolic acid using a casting method. The mechanical strength of SH-DM was investigated by an insertion test. In vivo transdermal absorption experiment was performed to evaluate the percutaneous absorption of SH-DM, sinomenine hydrochloride gel (SH-G) was used as a control. The results demonstrated that prepared SH-DM was morphologically intact with sufficient mechanical strength after inserting into aluminum foil and rat skin. The value of area under curve obtained after administration of SH-DM through transdermal in rabbits showed a significantly rise and was 1.99 times higher than that of SH-G, and the relative bioavailability value was 199.21%. These results showed that SH-DM enhanced bioavailability and permeability of SH.

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Multidrug release based on microneedle arrays filled with pH-responsive PLGA hollow microspheres

Cited by 128 publications

References 32 publications

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice

Localized sequence-specific release of a chemopreventive agent and an anticancer drug in a time-controllable manner to enhance therapeutic efficacy

Sinomenine hydrochloride-loaded dissolving microneedles enhanced its absorption in rabbits

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