Multidrug resistance gene expression response to cisplatin and 5FU treatment in hepatoma, prostate and colon cancer cells

Abstract

Variable expression of drug transporters at the plasma membrane of tumor cells contributed to drug resistance. The aim of the present study was to explore the expression levels of MRP2 and MRP3genes in Cisplatin and 5 fluorouracil (5FU) treated Hep3B, PC3 and HT29 cell lines. MTT assay was used to determine the cytotoxic effects of cisplatin and 5FU. The results from the MTT assay revealed that PC3 cell line is the most sensitive for cisplatin treatment presenting the lowest IC50 value (0.5 µg/ml) for 48h. 5FU treatment altered the proliferation of PC3 cells with the IC50 values 6.0 µg/ml for 48 h and 8.2 µg/ml for 72h. The mRNA expression levels of MRP2 and MRP3 were determined by quantitative reverse transcription polymerase chain reaction. qRT-PCR results revealed that while the expression levels of MRP3 mRNA was generally down regulated in all cell lines treated with cisplatin and 5FU, MRP2 was upregulated in PC3 and Hep3B cells.

Cisplatin binding to angiogenin protein: new molecular pathways and targets for the drug's anticancer activity

“…Under the investigated experimental conditions, the IC 50 value for CisPt was found at the concentrations of 50–100 μM (data not shown), according to the spread values ranging approximately from 20 to 130 μM found in the literature. 7,43,44…”

“…Under the investigated experimental conditions, the IC 50 value for CisPt was found at the concentrations of 50-100 μM (data not shown), according to the spread values ranging approximately from 20 to 130 μM found in the literature. 7,43,44 These divergences can be explained by considering that prostate cancer cells, and specifically the PC-3 line, generate high levels of ROS including H 2 O 2 and superoxide, in turn, and the ROS level increases with the aggressiveness of the cells. 44 The higher values of the IC 50 determined by the MTT assay should be considered in this context, where the conversion of the tetrazolium salt i.e.…”