Multienzyme Super-Dosing in Broiler Chicken Diets: The Implications for Gut Morphology, Microbial Profile, Nutrient Digestibility, and Bone Mineralization

Abstract

Optimizing gut health has a large impact on nutrient digestibility and bioavailability, and super-dosing feed enzymes may be one solution to achieve this. A 42-day grow-out trial was conducted using 192 Ross 308 broilers to determine if super-dosing Natuzyme at 0 g/t, 350 g/t, 700 g/t, and 1000 g/t dose rates could improve the gut morphology, alter the cecal microbial profile, enhance bone mineralization, and improve nutrient digestibility of a wheat–corn–soybean diet (six replicates per treatment, eight birds per pen). One bird per pen was slaughtered at day 42 and gut morphology, cecal microbial profile, and nutrient digestibility were studied. The addition of enzymes tended to increase the villus height in the duodenum, villus height, width, and crypt depth in the jejunum, and villus width and the number of goblet cells in the ileum. Microbial profiling revealed diverse communities; however, they did not significantly differ between treatment groups. Yet, 700 g/t Natuzyme promoted microbes belonging to the genus Romboutsia and Ruminococcus gauvreauii, while 1000 g/t Natuzyme promoted Barnesiella species. The nutrient digestibility demonstrated a significant improvement in all enzyme doses compared to the control. In conclusion, based on the outcomes of this study, a dose rate of 700 g/t Natuzyme is recommended to improve gut morphology and nutrient digestibility, and promote unique microbes which aid in feed efficiency.

Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes

“…Gibson et al found that via promoting lactic acid bacteria development in the hindgut, the hydrolysis products of enzymes may subtly inhibit the growth of some pathogens 73 . However, the current findings contradict those of Madigan-Stretton et al and Lourenco et al who revealed no significant prevalence for any connected bacterial species and no variations in microbial diversity across all multienzyme natuzyme treatment groups 48,74 .…”

“…Farmers must improve and maintain optimum digestive health in their birds since it leads to an enhanced performance index, enhanced feed utilization, and improved weight gain 47 . By breaking down macromolecules, modifying broiler gut physiology, and modifying the bacterial composition, multienzyme would enhance digestive health and increase the digestibility of nutrients in broilers 48 . This boost in growth performance may be triggered by the impacts of the reduction in the clostridia count and its secreted endotoxin, as well as the removal of the mycotoxins and their bad effects.…”

“…Madigan-Stretton et al discovered that villus height, width, and crypt depth were all improved in the duodenum by super-dosing multienzymes 48 . Villus width and the number of goblet cells in the jejunum were also increased.…”

Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers

“…On the other hand, conventional broilers in the current study were found to have a single amplicon sequence variant of Oscillibacter , Lachnospiracea NK4A136, and Ruminococcus gauvreauii that were differentially abundant in the ileum on day 7. Both Oscillibacter and Lachnospiracea NK4A136 are short chain fatty acid producers while Ruminococcus gauvreauii may be involved with feed efficiency ( Madigan-Stretton et al, 2020 ).…”

Effect of different levels of Labazyme supplementation on production and biochemical traits in quail

“…Poultry produce a variety of digestive enzymes. However, due to the presence of anti-nutritive factors that endogenous enzymes cannot combat, endogenous enzyme digestion alone leaves up to 25% of feed undigested (Celi et al, 2017;Madigan-Stretton et al, 2021). As a result, the significance of gut health in animal production highlights the need to improve food processing and nutrient utilization, as well as product performance and vitality (Burden, 2020).…”