Multiexposure laser speckle contrast imaging of the angiogenic microenvironment

Rege, Abhishek; Murari, Kartikeya; Seifert, Alan C.; Pathak, Arvind P.; Thakor, Nitish V.

doi:10.1117/1.3582334

Cited by 29 publications

(18 citation statements)

References 28 publications

(33 reference statements)

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“…Significant increase was observed in samples obtained from animals submitted to laser therapy alone and combined with S. chilensis. These results agree with the literature reporting that laser therapy stimulates angiogenesis [63,64]. In the present study, we observed that the same did not occur on the applications with the S. chilensis extract alone which probably indicates that the angiogenic effect is due to the action of the laser.…”

Section: Discussionsupporting

confidence: 92%

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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Therapies that accelerate the healing of burn injuries, improving the quality of life of the patient and reducing the cost of treatment are important. This study evaluated the effects of InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis leaves on burn wound healing in rats. Seventy-two rats were divided randomly into four groups: control untreated (C), treated with InGaP 670-nm laser with power density of 0.41 W/cm(2) and energy density of 4.93 J/cm(2) (L), treated with S. chilensis extract (S) and treated with S. chilensis extract and laser (LS). Second-degree burns were produced on the back of the animals with metal plate. Wound samples were collected on days 7, 14 and 21 of treatment for structural analysis, morphometry and Western blotting to quantify the expression of transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF). The results showed that InGaP laser irradiation at 670 nm alone and combined with extract of S. chilensis promoted significant tissue repair responses in this experimental model, increasing the number of fibroblasts, collagen fibres and newly formed blood vessels throughout the experimental period and decreasing the number of granulocytes in burn wounds of second degree in all treated groups. Exclusive treatment of burn wounds with the hydroalcoholic extract of S. chilensis provided similar quantitative results to those seen in the untreated group throughout the experimental period. Therefore, it was observed in the L and LS groups different responses in the expression of TGF-β1 and VEGF. The application of 670-nm laser alone or combined with the extract of S. chilensis promoted favourable responses in tissue repair of second-degree burns in this experimental model.

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Section: Discussionsupporting

confidence: 92%

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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“…The speckle ICTs are computed by fitting each curve to equation (2) and enable mapping of the flow dynamics by analyzing the characteristic shapes of these curves. It is important to note the inflections in the speckle variance curves, as multiexposure implementations with fewer exposures 36,37 congregated in one or two decades of exposure duration may insufficiently capture this relationship and thereby inaccurately sample the flow distributions present, especially after induced flow changes. A true MESI ICT image of flow is generated from the 15 single-exposure speckle images in Figure 2B.…”

Section: Resultsmentioning

confidence: 99%

Chronic Imaging of Cortical Blood Flow using Multi-Exposure Speckle Imaging

Kazmi

Parthasarthy

Song³

et al. 2013

J Cereb Blood Flow Metab

103

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Chronic imaging of cerebral blood flow (CBF) is an important tool for investigating vascular remodeling after injury such as stroke. Although techniques such as Laser Speckle Contrast Imaging (LSCI) have emerged as valuable tools for imaging CBF in acute experiments, their utility for chronic measurements or cross-animal comparisons has been limited. Recently, an extension to LSCI called Multi-Exposure Speckle Imaging (MESI) was introduced that increases the quantitative accuracy of CBF images. In this paper, we show that estimates of chronic blood flow are better with MESI than with traditional LSCI. We evaluate the accuracy of the MESI flow estimates using red blood cell (RBC) photographic tracking as an absolute flow calibration in mice over several days. The flow measures computed using the MESI and LSCI techniques were found to be on average 10% and 24% deviant (n ¼ 9 mice), respectively, compared with RBC velocity changes. We also map CBF dynamics after photo-thrombosis of selected cortical microvasculature. Correlations of flow dynamics with RBC tracking were closer with MESI (r ¼ 0.88) than with LSCI (r ¼ 0.65) up to 2 weeks from baseline. With the increased quantitative accuracy, MESI can provide a platform for studying the efficacy of stroke therapies aimed at flow restoration.

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“…Thinned skull preparation also has benefits like simpler surgical preparation and better control of intracranial pressure. For the thinned skull preparation, the standalone micro-imager can be updated with the multi-exposure strategy [29,30] for a robust CBF measurement.…”

Section: Multi-exposure Lsci and Thinned Skull Preparationmentioning

confidence: 99%

Chronic wide-field imaging of brain hemodynamics in behaving animals

Miao

Zhang

et al. 2016

Biomed. Opt. Express

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Abstract:Chronically monitoring cerebral activities in awake and freely moving status is very important in physiological and pathological studies. We present a novel standalone micro-imager for monitoring the cerebral blood flow (CBF) and total hemoglobin (HbT) activities in freely moving animals using the laser speckle contrast imaging (LSCI) and optical intrinsic signal (OIS) methods. A new cranial window method, using contact lens and wide field optics, is also proposed to achieve the chronic and wide-field imaging of rat's cerebral cortex. The hemodynamic activities of rats' cortex were measured for the first time without restriction of cables or fibers in awake and behaving animals. Chronic imaging showed the increase of CBF and HbT in motor cortex when the rats were climbing on the cage wall. Interestingly, the CBF activation of supplying vessel was smaller than that of parenchyma. Furthermore, after the climbing, CBF demonstrated fully return to the baseline while HbT showed a delayed recovery. The standalone micro-imager technology provides new possibilities of brain imaging in cognitive neuroscience studies. Kleinfeld, "Chronic optical access through a polished and reinforced thinned skull," Nat. Methods 7(12), 981-984 (2010). 20.

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Multiexposure laser speckle contrast imaging of the angiogenic microenvironment

Cited by 29 publications

References 28 publications

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Chronic Imaging of Cortical Blood Flow using Multi-Exposure Speckle Imaging

Chronic wide-field imaging of brain hemodynamics in behaving animals

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