Multifaceted dialogue between graft and host in neurotransplantation

Ourednik, Václav; Ourednik, Jitka

doi:10.1002/jnr.20037

Cited by 19 publications

(9 citation statements)

References 98 publications

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“…Reverse transcription was performed with SuperScript III (Invitrogen Life Technologies) and an Oligo (dT) [12][13][14][15][16][17][18] primer. Four micrograms of RNA were added into a final volume of 21-mL solution containing 10 mM deoxynucleotide triphosphate mix, 10· RT buffer, 25 mM MgCl 2 , 0.1 M dithiothreitol, RNase inhibitor, and RNase H. Six micrograms of RT product were used for the PCR amplification in a final volume of 50 mL containing 2.5 mM deoxynucleotide triphosphate, 25 mM MgCl 2 , upstream or downstream primers (nestin and GDNF), and Taq DNA polymerase (Invitrogen Life Technologies).…”

Section: Rna Extraction and Semiquantitative Polymerase Chain Reactiomentioning

confidence: 99%

“…For instance, locomotor improvements have been demonstrated in spinal cord-injured mice and primates (13)(14)(15). However, no single therapeutic methodology has been proven effective enough for significant functional recovery because of difficulties such as posttransplantation cellular lineage restriction (16), variable host responses to the engraftment (17,18), and unfavorable hostile environment for the implanted cells.…”

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confidence: 99%

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A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

Lo¹,

Hsu²,

et al. 2008

J Nucl Med

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This aim of our study was to evaluate a novel cell-based therapy for contusion spinal cord injury (SCI) using embryonic-derived NIH3T3 cells, which endogenously express glial cell line-derived neurotrophic factor (GDNF). Methods: Proliferation and differentiation of transplanted NIH3T3 cells and their anti-apoptotic effects were examined after their engraftment into the spinal cords of Long-Evans rats subjected to acute SCI at the T10 vertebral level by a New York University impactor device. NIH3T3 cells were initially engineered to contain dual reporter genes, namely thymidine kinase (T) and enhanced green fluorescence protein (G), for in vivo cell tracking by both nuclear and fluorescence imaging modalities. Results: Planar and fluorescence imaging demonstrated that transplanted NIH3T3-TG cells at the L1 vertebral level migrated 2 cm distal to the injury site as early as 2 h, and the signals persisted for 48 h after SCI. The expression of GDNF by NIH3T3-TG cells was then confirmed by immunohistochemical analysis both in vitro and in vivo. GDNFsecreting NIH3T3-TG transplant provided anti-apoptotic effects in the injured cord over the period of 3 wk. Finally, NIH3T3-TG cells cultured under neuronal differentiation medium exhibited both morphologic and genetic resemblance to neuronal cells. Conclusion: GDNF-secreting NIH3T3-TG cells in combination with molecular imaging could be a platform for developing therapeutic tools for acute SCI.

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Section: Rna Extraction and Semiquantitative Polymerase Chain Reactiomentioning

confidence: 99%

mentioning

confidence: 99%

A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

Lo¹,

Hsu²,

et al. 2008

J Nucl Med

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“…The loss of neurons characterizes many neurodegenerative diseases, such as PD, Alzheimer's disease, and Huntington's disease, and injuries such as stroke, traumatic brain injury, and spinal cord injury. Furthermore, the CNS is known to have a poor capacity for generating new neurons and oligodendrocytes after injury or degeneration, with the adult and aged CNS, in particular, possessing a prevailing non-neurogenic and growth-inhibitory milieu that negatively influences these processes [143,144]. For these reasons, therapies endeavoring to replace the damaged or missing neurons and restore some degree of function have been the focus of much attention.…”

Section: Overview Of Parkinson's Diseasementioning

confidence: 99%

Mesenchymal Stem Cell-Based Therapies for Parkinson’s Disease: Progress, Controversies and Lessons for the Future

Khoo¹,

Tao²,

D.F.³

2011

J Stem Cell Res Ther

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Scientific and clinical research interest in mesenchymal stem cells (MSCs) has increased exponentially since the identification of MSCs in the bone marrow. It is now recognized that MSCs possess the in vitro characteristics of stem cells with the abilities to proliferate, symmetrically divide, and produce multi-lineage mesodermal derivatives, making MSCs attractive candidates for use in potential cellular therapies. Furthermore, MSCs can be relatively easily isolated and expanded in culture with low tumorigenicity and teratoma formation, and have been reported to display immunomodulatory properties that may be advantageous in clinical transplantation. Discovery of the ability of MSCs to differentiate into cells of non-mesodermal tissues, particularly neural cells, has also raised the possibility of utilizing MSCs in regenerative and reparative therapies for neurological disorders. However, a number of hurdles remain to be resolved, including conflicting findings concerning the capacity of MSCs to suppress immune responses and contribute to multiple tissue lineages, highlighting the need for a greater understanding of mechanisms underlying the observed phenomena. In this review we will discuss: (1) recent advances in our understanding of MSC plasticity/transdifferentiation and immunomodulatory properties; (2) evidence for cell-based therapies, in particular MSC-based therapies for Parkinson's disease; and (3) current challenges and potential strategies for the utilization of MSCs in the treatment of Parkinson's disease.

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“…Early during intrauterine life, germinative regions found in the segregation of NSCs grafted into the developing or functionally compromised postnatal CNS harboring NSCs represent a relatively large part of the developing CNS but diminish progressively during CNS (48,50). While studying the behavior of a single clone of human NSCs (hNSCs) grafted into the brain of a midontogeny (Fig.…”

Section: Introductionmentioning

confidence: 99%

Plasticity of the Central Nervous System and Formation of “Auxiliary Niches” after Stem Cell Grafting: An Essay

Ourednik

2007

Cell Transplant

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It is hoped that stem cell biology will play a major role in the treatment of a number of so far incurable diseases via transplantation therapy. Today, we know that neural stem cell grafts not only represent a valuable source of missing cells and molecules for the host nervous system, but they also bring with them biological principles and processes assuring tissue plasticity and homeostasis found in early development and in postnatal neurogenic areas. In this review, we discuss the potential of grafted neural stem/progenitor cells to induce plasticity in the adult diseased brain by mimicking the cellular and molecular processes governing the biology of endogenous stem cell niches. If confirmed, such anlagen of "auxiliary niches" could help us to optimize intercellular communication in donor cell-initiated networks of graft-host interactions and to "rejuvenate" the adult nervous system in its response to disease and injury.

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Multifaceted dialogue between graft and host in neurotransplantation

Cited by 19 publications

References 98 publications

A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

A Novel Cell-Based Therapy for Contusion Spinal Cord Injury Using GDNF-Delivering NIH3T3 Cells with Dual Reporter Genes Monitored by Molecular Imaging

Mesenchymal Stem Cell-Based Therapies for Parkinson’s Disease: Progress, Controversies and Lessons for the Future

Plasticity of the Central Nervous System and Formation of “Auxiliary Niches” after Stem Cell Grafting: An Essay

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