Multifunctional Au nanoclusters for targeted bioimaging and enhanced photodynamic inactivation of Staphylococcus aureus

Khlebtsov, Boris N.; Tuchina, Elena S.; Tuchin, Valery V.; Khlebtsov, Nikolai G.

doi:10.1039/c5ra11713e

Cited by 46 publications

(32 citation statements)

References 73 publications

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“…This work is meaningful for providing a simple, one-pot approach of synthesizing highly fluorescent AuNCs. Recently, Boris and coworkers have developed a novel synthesis method to prepare protein-directed AuNCs [8]. As shown in Fig.…”

Section: Proteinsmentioning

confidence: 99%

“…Numerous studies demonstrated that AuNCs also displayed similar solvatochromic properties. Chen's group reported photoluminescence properties of Au 8 NCs when exposed to different organic solvents and further explored the solvatochromic effect of Au 8 NCs protected by different ligands. They confirmed that the solvatochromic effect came from the rich surface properties of Au 8 NCs [30].…”

Section: Solvatochromic Propertiesmentioning

confidence: 99%

“…During the synthesis of NCs, Au 3? ions are mainly reduced by the 21 tyrosine residues in BSA through the phenolic groups. Tseng's group presented a novel approach for preparing highly fluorescent Au 8 NCs and Au 25 NCs by using lysozyme type VI (Lys VI) at pH 3 and pH 12, [43] respectively [49]. Inspired by this finding, researchers try to explore other proteins as efficient bioscaffolds for synthesizing AuNCs such as insulin [50], transferrin-family proteins [51,52], horseradish peroxidase [53], pepsin [54], denatured BSA [55], ribonuclease A [56], trypsin [57], DNase I [58], ovalbumin [59], and egg white [60].…”

Section: Proteinsmentioning

confidence: 99%

“…Poly (amidoamine) (PAMAM) and poly (propyleneimine) (PPI) have been frequently utilized as macromolecule templates to produce AuNCs based on the capability of sequestering metal ions from aqueous or methanol solutions. Zheng et al synthesized PMMA dendrimer-stabilized Au 8 NCs featuring strong blue luminescence with excitation and emission maxima at 384 and 450 nm [44].…”

Section: Dendrimers and Polymersmentioning

confidence: 99%

“…Today, highly fluorescent AuNCs can be fabricated with atomically precise sizes and tunable optical properties. For biomedical applications, AuNCs have been synthesized by using biocompatible capping ligands, including DNA [6], glutathione (GSH) [7], bovine serum albumin (BSA) [8], and other proteins [9]. Such novel properties make fluorescent AuNCs an ideal nanomaterial for promising applications in biosensing [10], imaging [11], and catalysis [12].…”

Section: Introductionmentioning

confidence: 99%

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Fluorescent Gold Nanoclusters: Promising Fluorescent Probes for Sensors and Bioimaging

Wang

Zhu

2017

J. Anal. Test.

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Fluorescent gold nanoclusters (AuNCs) have recently emerged as a novel kind of promising fluorescent probes for high-performance sensors and bioimaging because of their ultrasmall size (\3 nm), strong luminescence, good photostability, and excellent biocompatibility. Over the past decade, we have witnessed growing popularity of AuNCs in analytical applications and enormous efforts have been devoted to their development. In this review, we provide an update on recent advances in the development of AuNCs in terms of physicochemical properties, synthesis strategies, and bioapplications. The optical, electrochemical, catalytical, and solvatochromic properties of AuNCs are first summarized, which are followed by different ligands or template-assisted controllable synthetic methods. Afterwards functionalization of AuNCs is described in terms of ligand exchange, bioconjugation, and noncovalent interaction. We then focus on the applications of AuNCs as fluorescent probes for detection of metal ions, inorganic anions, small biomolecules, proteins, nucleic acids, drug molecules, pH, and temperature. We also summarize the usage of metal NCs in cellular and in vivo targeting and imaging. Finally, we conclude with a brief look at the future challenges and prospects of the development of AuNCs.

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Section: Proteinsmentioning

confidence: 99%

Section: Solvatochromic Propertiesmentioning

confidence: 99%

Section: Proteinsmentioning

confidence: 99%

Section: Dendrimers and Polymersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fluorescent Gold Nanoclusters: Promising Fluorescent Probes for Sensors and Bioimaging

Wang

Zhu

2017

J. Anal. Test.

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Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

Mok

Loh

et al. 2016

Adv Healthcare Materials

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thiolate-protected, [ 3 ] protein/peptide-templated, [ 4 ] and DNA-templated [ 5 ] ones. The last two types of metal NCs use biomolecules as templates to direct the synthesis (thus termed as biotemplated metal NCs), which afford more attractive features such as good aqueous solubility, superb biocompatibility, and versatile surface chemistries, [ 6 ] in addition to their ultrasmall size and superior photoluminescent optical properties. These desirable features render biotemplated metal NCs as promising candidates for various biomedical applications like imaging, [ 7 ] sensing, [ 7b,8 ] delivery [ 9 ] and therapeutics. [ 10 ] Theranostics is a revolutionary treatment strategy proposed by Funkhouser in 1998, which combines accurate diagnosis and targeted therapeutics. [ 11 ] From the list of individal biomedical applications mentioned earlier, a combination of sensing and imaging modalities would be fundamental for disease diagnosis. [ 12 ] In the same vein, image-guided targetedtherapy allows for real-time in vivo monitoring of the pharmacokinetics and pharmacodynamics of the therapeutic agent to enhance treatment effi ciency. [ 13 ] Through the ingenious combination of selective modalities, a highly potent and personalized theranostic agent could be developed. In particular, NC-based systems are poised to be the next versatile theranostic platform. This is because the intrinsic photoluminescence of the noble metal core can be harnessed for fl uorescence imaging, and tailor-made to exhibit unique optical properties for therapy, such as the generation of singlet oxygen [ 14 ] to substitute organicbased photosensitizers for possible photodynamic cancer treatments. In addition to the metal core, the biomolecular ligand shell surrounding the metal NC core can be easily functionalized with biorecognition moieties for sensing and targeted delivery. Due to the large surface to volume ratio of metal NCs, it can also serve as a reservoir sink to load multiple drugs/ genes and be modifi ed with sensing/imaging agents to imbue both therapeutic and diagnostic functions.The selective marriage of respective modalities to form theranostic biotemplated metal NCs will have a far-reaching impact on the future of medicine and healthcare. There are large amounts of work that use randomly picked natural biomolecules as templates to synthesize metal NCs for biomedical

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Aggregation‐Induced Emission (AIE) in Ag−Au Bimetallic Nanocluster

Jin

Liu

et al. 2018

Chemistry A European J

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Herein we report the synthesis and structure determination of a non-fluorescent Au Ag (dppm) (SAdm) (BPh ) (dppm=bis(diphenylphosphino)methane and HSAdm=1-adamantane mercaptan) nanocluster in methanol with extremely strong AIE when aggregating to the solid state (i.e., film or crystal). This phenomenon was rarely reported in structural determined noble metal nanoclusters. The extended X-ray absorption fine structure (EXAFS) measurement ruled out the hypothesis that the luminescence originated from the structure change in different states. Besides, the crystal structure (determined by X-ray diffraction) revealed that the tightly combined left- and right-handed enantiomers induced the strong restriction of intramolecular motions (RIM), which may have an impact on aggregation-induced emission.

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Multifunctional Au nanoclusters for targeted bioimaging and enhanced photodynamic inactivation of Staphylococcus aureus

Abstract: A novel nanocluster platform is developed to combine intense red fluorescence of Au25–BSA nanoclusters (QY ∼ 14%), biospecific binding to S. aureus due to human antistaphylococcal IgG, and photodynamic inactivation due to photosensitizer Photosens™.

Cited by 46 publications

References 73 publications

Fluorescent Gold Nanoclusters: Promising Fluorescent Probes for Sensors and Bioimaging

Fluorescent Gold Nanoclusters: Promising Fluorescent Probes for Sensors and Bioimaging

Rational Design of Biomolecular Templates for Synthesizing Multifunctional Noble Metal Nanoclusters toward Personalized Theranostic Applications

Aggregation‐Induced Emission (AIE) in Ag−Au Bimetallic Nanocluster

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