Multifunctional biosynthesized silver nanoparticles exhibiting excellent antimicrobial potential against multi-drug resistant microbes along with remarkable anticancerous properties

Jha, Diksha; Thiruveedula, Prasanna Kumar; Pathak, Rajiv; Kumar, Bipul; Gautam, Hemant K.; Agnihotri, Shrish; Sharma, Ashwani; Kumar, Pradeep

doi:10.1016/j.msec.2017.07.011

Cited by 44 publications

(14 citation statements)

References 48 publications

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“…The synthesized silver and gold nanoparticles when tested for their free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl and antimicrobial activity against E. coli, S. aureus, Aspergillus flavus and Candida albicans showed high antioxidant and antimicrobial activities. In a similar study, Jha et al [25] recently The charges on chitosan backbone can be created by protonation of the -NH 2 groups in acid solution or by structural modification (i.e., methylation, sulfonation, etc.) [1,18].…”

Section: Introductionmentioning

confidence: 93%

“…The synthesized silver and gold nanoparticles when tested for their free radical scavenging activity against 1,1-diphenyl-2-picryl-hydrazyl and antimicrobial activity against E. coli, S. aureus, Aspergillus flavus and Candida albicans showed high antioxidant and antimicrobial activities. In a similar study, Jha et al [25] recently demonstrated that highly stable silver nanoparticles suitable for therapeutic applications can be synthesized using the extracts of Citrus maxima plant. Several of such reports can be found in the literature [7,[21][22][23]26,27].…”

Section: Introductionmentioning

confidence: 96%

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Preparation of Silver/Chitosan Nanofluids Using Selected Plant Extracts: Characterization and Antimicrobial Studies against Gram-Positive and Gram-Negative Bacteria

et al. 2020

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Chitosan/silver nanofluids were prepared using Phoenix dactylifera (DPLE) or Rumex vesicarius (HEL) extracts as the reducing agent, characterized using Fourier-transform infrared spectroscopy (FTIR), ultraviolet–visible (UV-vis), X-ray diffraction (XRD), and transmission electron microscope (TEM). The antimicrobial effect of the nanofluids against Gram positive, Bacillus licheniformis, Staphylococcus haemolyticus, Bacillus cereus, and Micrococcus luteus, and Gram-negative Pseudomonas aeruginosa, Pseudomonas citronellolis, and Escherichia coli bacteria has been studied. The nanoparticles were polydispersed in the chitosan matrix and are highly stable. The zeta potential of the silver nanoparticles in DPLE- and HEL-mediated composites is +46 mV and +56 mV, respectively. The FTIR results reveal that the free carboxylate groups in the plant biomaterial took part in stabilization process. HEL is a stronger reducing agent than DPLE and nanoparticles generated with HEL are smaller (8.0–36 nm) than those produced with DPLE (10–43 nm). DPLE- and HEL-mediated composites effectively inhibit the growth of the studied bacteria but HEL-mediated composite exhibited higher effect. The higher antimicrobial activity of HEL-mediated composite is linked to the smaller nanoparticles. The foregoing results indicate that HEL extract can be used in the green production of potential antimicrobial chitosan/silver nanofluids for biomedical and packaging applications.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 96%

Preparation of Silver/Chitosan Nanofluids Using Selected Plant Extracts: Characterization and Antimicrobial Studies against Gram-Positive and Gram-Negative Bacteria

et al. 2020

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“…1 Silver nanoparticles (Ag-NPs) have obtained considerable attention due to their attractive physicochemical properties 2,3 and remarkable antimicrobial effects exhibited against Gramnegative and Gram-positive multidrug-resistant bacteria. 4 The bactericidal effect of silver in its various chemical forms has been exploited since ancient civilizations; 5 during the 18th century, silver was commonly used to treat infected ulcers, and more recently, in the early 1920s, Ag-NPs were included for wound treatment by the US Food and Drug Administration 6 due to their strong toxicity against a wide range of microorganisms. [7][8][9] There are various mechanisms that have been linked to the toxicity of silver compounds against microorganisms, among the most common are direct disruptive interactions with the cell membrane and DNA 2 and the trigger of biochemical cascades that lead to free radical production, altering cell membrane permeability.…”

Section: Introductionmentioning

confidence: 99%

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

Escárcega‐González¹,

Garza-Cervantes²,

Vázquez-Rodríguez³

et al. 2018

IJN

140

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IntroductionOne of the main issues in the medical field and clinical practice is the development of novel and effective treatments against infections caused by antibiotic-resistant bacteria. One avenue that has been approached to develop effective antimicrobials is the use of silver nanoparticles (Ag-NPs), since they have been found to exhibit an efficient and wide spectrum of antimicrobial properties. Among the main drawbacks of using Ag-NPs are their potential cytotoxicity against eukaryotic cells and the latent environmental toxicity of their synthesis methods. Therefore, diverse green synthesis methods, which involve the use of environmentally friendly plant extracts as reductive and capping agents, have become attractive to synthesize Ag-NPs that exhibit antimicrobial effects against resistant bacteria at concentrations below toxicity thresholds for eukaryotic cells.PurposeIn this study, we report a green one-pot synthesis method that uses Acacia rigidula extract as a reducing and capping agent, to produce Ag-NPs with applications as therapeutic agents to treat infections in vivo.Materials and methodsThe Ag-NPs were characterized using transmission electron microscopy (TEM), high-resolution TEM, selected area electron diffraction, energy-dispersive spectroscopy, ultraviolet–visible, and Fourier transform infrared.ResultsWe show that Ag-NPs are spherical with a narrow size distribution. The Ag-NPs show antimicrobial activities in vitro against Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and a clinical multidrug-resistant strain of P. aeruginosa) and Gram-positive (Bacillus subtilis) bacteria. Moreover, antimicrobial effects of the Ag-NPs, against a resistant P. aeruginosa clinical strain, were tested in a murine skin infection model. The results demonstrate that the Ag-NPs reported in this work are capable of eradicating pathogenic resistant bacteria in an infection in vivo. In addition, skin, liver, and kidney damage profiles were monitored in the murine infection model, and the results demonstrate that Ag-NPs can be used safely as therapeutic agents in animal models.ConclusionTogether, these results suggest the potential use of Ag-NPs, synthesized by green chemistry methods, as therapeutic agents against infections caused by resistant and nonresistant strains.

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“…S. aureus and B. cereus. The results of zone of inhibition and minimum inhibitory concentration assays [43][44][45][46] for both are presented in Tables 3 and 4. These results revealed the activity of the projected conjugate polymers and it was observed that the conjugated polymers were quite effective in inhibiting the bacterial growth.…”

Section: Antibacterial Activity Of Lmwp-s and Hmwp-s Conjugatesmentioning

confidence: 99%

Bifunctionally engineered polyethylenimines as efficient DNA carriers and antibacterials against resistant pathogens

et al. 2018

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In this study, we have designed and developed two series of bifunctional conjugates by tethering polyethylenimine with streptomycin. By varying the amount of streptomycin, conjugates, polyethylenimine-streptomycin, have been synthesized and characterized spectroscopically. Gel electrophoresis assay revealed a slight decrease in the cationic charge density on the conjugates as these retarded the mobility of pDNA at higher w/w ratios. Further, transfection studies showed that both the series of conjugates transfected the mammalian cells efficiently with low-molecular weight polyethylenimine-streptomycin conjugates were more competent (∼9-fold enhancement with respect to native bPEI) exhibiting high cell viability too. Besides, both the series of conjugates displayed excellent antibacterial activity on pathogenic bacteria, even better than native streptomycin on resistant strains. Altogether, these results ensure the promising potential of the projected bifunctional conjugates as safe and efficient gene delivery vectors as well as antibacterials for future biomedical applications.

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Multifunctional biosynthesized silver nanoparticles exhibiting excellent antimicrobial potential against multi-drug resistant microbes along with remarkable anticancerous properties

Cited by 44 publications

References 48 publications

Preparation of Silver/Chitosan Nanofluids Using Selected Plant Extracts: Characterization and Antimicrobial Studies against Gram-Positive and Gram-Negative Bacteria

Preparation of Silver/Chitosan Nanofluids Using Selected Plant Extracts: Characterization and Antimicrobial Studies against Gram-Positive and Gram-Negative Bacteria

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

Bifunctionally engineered polyethylenimines as efficient DNA carriers and antibacterials against resistant pathogens

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