Multifunctional Complexes Based on Photoluminescent Upconversion Nanoparticles for Theranostics of the HER2-Positive Tumors

Guryev, Evgenii L.; Smyshlyaeva, A S; Shilyagina, Natalia Yu.; Shanwar, Samah; Kostyuk, A. B.; Shulga, A. A.; Коновалова, Е. В.; Zvyagin, Andrei V.; Deyev, Sergey M.; Петров, Р. В.

doi:10.1134/s160767292002009x

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…A good alternative to traditional organic fluorophores is using various recently developed nanomaterials, such as semiconductor quantum dots, porous silicon semiconductor nanoparticles, carbon dots, gold nanoclusters, etc. [88][89][90][91][92][93].…”

Section: Optical Methods Of Immunodetection Based On Nanomaterialsmentioning

confidence: 99%

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

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The recognition of biomolecules is crucial in key areas such as the timely diagnosis of somatic and infectious diseases, food quality control, and environmental monitoring. This determines the need to develop highly sensitive display devices based on the achievements of modern science and technology, characterized by high selectivity, high speed, low cost, availability, and small size. Such requirements are met by biosensor systems—devices for reagent-free analysis of compounds that consist of a biologically sensitive element (receptor), a transducer, and a working solution. The diversity of biological material and methods for its immobilization on the surface or in the volume of the transducer and the use of nanotechnologies have led to the appearance of an avalanche-like number of different biosensors, which, depending on the type of biologically sensitive element, can be divided into three groups: enzyme, affinity, and cellular/tissue. Affinity biosensors are one of the rapidly developing areas in immunoassay, where the key point is to register the formation of an antigen–antibody complex. This review analyzes the latest work by Russian researchers concerning the production of molecules used in various immunoassay formats as well as new fundamental scientific data obtained as a result of their use.

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Section: Optical Methods Of Immunodetection Based On Nanomaterialsmentioning

confidence: 99%

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

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show abstract

“…In order to ensure an additional therapeutic modality of nanophosphors, their surface was modified with the DARPin 9_29 protein fused with a low-immunogenicity fragment of the pseudomonad exotoxin A, LoPE, using genetic engineering techniques [ 89 ]. The resulting DARP-LoPE protein possesses all the qualities needed for theranostics: it is capable of targeted interaction with target cells and is cytotoxic upon binding to cells.…”

Section: Darpins As a Tool For The Targeted Delivery Of Nanoparticlesmentioning

confidence: 99%

“…DARP-LoPE immunotoxin, a targeting cytotoxic protein, was conjugated to the surface of nanosized anti-Stokes phosphors using carbodiimide and an intermediate linker, polyethylene glycol. The as-synthesized nanosized phosphors were coated with PMAO, an alternating maleic anhydride–1-octadecene copolymer, and polyethylene glycol to ensure a greater colloidal stability [ 89 ]. The cytotoxicity of the targeting nanosized phosphor–PEG–DARP–LoPE complexes was studied for SK-BR-3-Kat cells.…”

Section: Darpins As a Tool For The Targeted Delivery Of Nanoparticlesmentioning

confidence: 99%

Artificial Scaffold PolypeptidesAs an Efficient Tool for the Targeted Delivery of Nanostructures In Vitro and In Vivo

Shipunova

Deyev

2022

Acta Naturae

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The use of traditional tools for the targeted delivery of nanostructures, such as antibodies, transferrin, lectins, or aptamers, often leads to an entire range of undesirable effects. The large size of antibodies often does not allow one to reach the required number of molecules on the surface of nanostructures during modification, and the constant domains of heavy chains, due to their effector functions, can induce phagocytosis. In the recent two decades, targeted polypeptide scaffold molecules of a non-immunoglobulin nature, antibody mimetics, have emerged as much more effective targeting tools. They are small in size (320 kDa), possess high affinity (from subnano- to femtomolar binding constants), low immunogenicity, and exceptional thermodynamic stability. These molecules can be effectively produced in bacterial cells, and, using genetic engineering manipulations, it is possible to create multispecific fusion proteins for the targeting of nanoparticles to cells with a given molecular portrait, which makes scaffold polypeptides an optimal tool for theranostics.

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“…The possibility of using UCNPs for specific visualization of tumor cells and experimental tumors has been demonstrated in [ 190 , 203 , 204 , 205 , 206 ]. Attachment of the bifunctional targeted toxins specific to the tumor cells of a certain molecular profile to biocompatible UCNPs makes it possible to open the therapeutic potential of the designed complexes [ 207 , 208 ] and use the advantages of combined therapy. It has been shown that the efficacy of therapeutic modules (β-emitter and targeted toxin) increases by more than two orders of magnitude when they are used as parts of a theranostic nanocomplex to attack tumor cells [ 209 ].…”

Section: Up-conversion Nanoparticlesmentioning

confidence: 99%

Photoluminescent Nanomaterials for Medical Biotechnology

et al. 2021

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Creation of various photoluminescent nanomaterials has significantly expanded the arsenal of approaches used in modern biomedicine. Their unique photophysical properties can significantly improve the sensitivity and specificity of diagnostic methods, increase therapy effectiveness, and make a theranostic approach to treatment possible through the application of nanoparticle conjugates with functional macromolecules. The most widely used nanomaterials to date are semiconductor quantum dots; gold nanoclusters; carbon dots; nanodiamonds; semiconductor porous silicon; and up-conversion nanoparticles. This paper considers the promising groups of photoluminescent nanomaterials that can be used in medical biotechnology: in particular, for devising agents for optical diagnostic methods, sensorics, and various types of therapy.

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Multifunctional Complexes Based on Photoluminescent Upconversion Nanoparticles for Theranostics of the HER2-Positive Tumors

Cited by 7 publications

References 14 publications

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Artificial Scaffold PolypeptidesAs an Efficient Tool for the Targeted Delivery of Nanostructures In Vitro and In Vivo

Photoluminescent Nanomaterials for Medical Biotechnology

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