Multifunctional double network hydrogel film for skin wound healing

Abstract

Skin wound management is an important issue in the medical community. Herein, we report a multifunctional film with many advanced features as a dressing for the healing of wounds. The dressing was made using chitosan and poly(acrylamide) (pAAm) hydrogel in order to form a double network (DN) hydrogel film. Compared to the pure chitosan hydrogel, which is fragile, the added pAAm network conferred excellent mechanical capability to the composite film, making it flexible and easy to treat. By further doping the hydrogel with carbon nanotubes and drugs, the film could promote wound healing. Animal experiments demonstrated that the DN hydrogel film could reduce inflammation and promote tissue regeneration. Thus, this film is promising as a treatment for wound healing.

A comprehensive review on the applications of carbon-based nanostructures in wound healing: from antibacterial aspects to cell growth stimulation

“…In the case of the DN + CNTs + VEGF hydrogels, the wound surface size treated by this group was approximately 12% larger than that treated with PBS after 7 days. 177 Safaei…”

“…In the case of the DN + CNTs + VEGF hydrogels, the wound surface size treated by this group was approximately 12% larger than that treated with PBS after 7 days. 177 Safaei et al proposed a one-step method to fabricate optical core–shell microfiber textiles by combining SWCNTs as a sensor layer to monitor oxidative stress and light based on the real-time hydrogen peroxide concentration in in vitro wounds. Hydrogen peroxide as an intracellular ROS messenger stimulates key wound healing stages including angiogenesis.…”

Carbon Nanotubes-Based Hydrogels for Bacterial Eradiation and Wound-Healing Applications

“…The presence of CNT enhanced the interaction among cells and promoted cell proliferation. Figure 5d represents the hematoxylin and eosin (H&E) staining of regenerated granulation tissues from wound sites in different groups and their statistical analysis of tissue thickness [139].…”

“…The H&E analysis confirmed the healing process by showing the high speed of hair follicle recovery, and finally, the skin surface was smooth. [137], (c) In vivo wound-healing model evaluation of MNH hydrogels and observation of treatment in presence of S. aureus infection for 12 days [138], (d) (i) H&E staining of different groups showing the progress in thickness of skin, (ii) respective statistical analysis of average connective tissue thickness in different groups [139], (e) Schematic of chemoattractantinduced cell migration assay using a modified Boyden Chamber experiment. The addition of CNT increased the porosity of the hydrogel and cell migration (left).…”

“…Figure5. (a) Schematic representation of wound-healing phases in cutaneous wound-hemostasis, inflammation, proliferation, and remodeling[2], (b) (i) Scheme representation of mouse-tail amputation model, (ii) bloodstain photograph of mouse tail in blank and hydrogel group, (iii) quantitative data of blood loss (n = 5), ** p < 0.01[137], (c) In vivo wound-healing model evaluation of MNH hydrogels and observation of treatment in presence of S. aureus infection for 12 days[138], (d) (i) H&E staining of different groups showing the progress in thickness of skin, (ii) respective statistical analysis of average connective tissue thickness in different groups[139], (e) Schematic of chemoattractantinduced cell migration assay using a modified Boyden Chamber experiment. The addition of CNT increased the porosity of the hydrogel and cell migration (left).…”