Multifunctional lanthanide and silver ion co-doped nano-chlorapatites with combined spectroscopic and antimicrobial properties

Wiglusz, Rafał J.; Drulis-Kawa, Zuzanna; Pązik, Robert; Zawisza, Katarzyna; Dorotkiewicz-Jach, Agata; Roszkowiak, Justyna; Nédélec, Jean-Marie

doi:10.1039/c5dt00046g

Cited by 16 publications

(10 citation statements)

References 24 publications

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“…As investigated by Iconaru et al [ 75 ], there was no antimicrobial activity of Eu 3+ -doped hydroxyapatites detected against E. coli 25922. Our previous study also showed no effect of Eu 3+ -doped hydroxyapatites and chlorapatites against Gram-negative bacteria [ 76 , 77 ]. However, these materials differed in the concentration of Eu 3+ ions or contained other co-dopants, such as Sr 2+ ions.…”

Section: Resultsmentioning

confidence: 99%

Multifunctionality of Nanosized Calcium Apatite Dual-Doped with Li+/Eu3+ Ions Related to Cell Culture Studies and Cytotoxicity Evaluation In Vitro

et al. 2021

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Li+/Eu3+ dual-doped calcium apatite analogues were fabricated using a microwave stimulated hydrothermal technique. XRPD, FT-IR, micro-Raman spectroscopy, TEM and SAED measurements indicated that obtained apatites are single-phased, crystallize with a hexagonal structure, have similar morphology and nanometric size as well as show red luminescence. Lithium effectively modifies the local symmetry of optical active sites and, thus, affects the emission efficiency. Moreover, the hydrodynamic size and surface charge of the nanoparticles have been extensively studied. The protein adsorption (lysozyme, LSZ; bovine serum albumin, BSA) on the nanoparticle surface depended on the type of cationic dopant (Li+, Eu3+) and anionic group (OH−, Cl−, F−) of the apatite matrix. Interaction with LSZ resulted in a positive zeta potential, and the nanoparticles had the lowest hydrodynamic size in this protein medium. The cytotoxicity assessment was carried out on the human osteosarcoma cell line (U2OS), murine macrophages (J774.E), as well as human red blood cells (RBCs). The studied apatites were not cytotoxic to RBCs and J774.E cells; however, at higher concentrations of nanoparticles, cytotoxicity was observed against the U2OS cell line. No antimicrobial activity was detected against Gram-negative bacteria with one exception for P. aeruginosa treated with Li+-doped fluorapatite.

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Section: Resultsmentioning

confidence: 99%

Multifunctionality of Nanosized Calcium Apatite Dual-Doped with Li+/Eu3+ Ions Related to Cell Culture Studies and Cytotoxicity Evaluation In Vitro

et al. 2021

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“…Although the last ionic additives incorporable into CAP will soon be off the list in terms of being integrated into CAP and limitedly characterized for their properties, it will be a while before even the tip of the iceberg of possibilities achievable through the synergy amongst different ionic dopants is revealed. Some of such simultaneous incorporations of two or more types of ions into CAP, such as silver and lanthanides [ 75 ], zinc and magnesium [ 76 ], selenite and manganese [ 77 ], europium and gadolinium [ 78 ], have already been tested for their multifunctional properties.…”

Section: Cap As a Foreign Ion Accommodatormentioning

confidence: 99%

Calcium Phosphate as a Key Material for Socially Responsible Tissue Engineering

Uskoković

2016

Materials

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Socially responsible technologies are designed while taking into consideration the socioeconomic, geopolitical and environmental limitations of regions in which they will be implemented. In the medical context, this involves making therapeutic platforms more accessible and affordable to patients in poor regions of the world wherein a given disease is endemic. This often necessitates going against the reigning trend of making therapeutic nanoparticles ever more structurally complex and expensive. However, studies aimed at simplifying materials and formulations while maintaining the functionality and therapeutic response of their more complex counterparts seldom provoke a significant interest in the scientific community. In this review we demonstrate that such compositional simplifications are meaningful when it comes to the design of a solution for osteomyelitis, a disease that is in its natural, non-postoperative form particularly prevalent in the underdeveloped parts of the world wherein poverty, poor sanitary conditions, and chronically compromised defense lines of the immune system are the norm. We show that calcium phosphate nanoparticles, which are inexpensive to make, could be chemically designed to possess the same functionality as a hypothetic mixture additionally composed of: (a) a bone growth factor; (b) an antibiotic for prophylactic or anti-infective purposes; (c) a bisphosphonate as an antiresorptive compound; (d) a viral vector to enable the intracellular delivery of therapeutics; (e) a luminescent dye; (f) a radiographic component; (g) an imaging contrast agent; (h) a magnetic domain; and (i) polymers as viscous components enabling the injectability of the material and acting as carriers for the sustained release of a drug. In particular, calcium phosphates could: (a) produce tunable drug release profiles; (b) take the form of viscous and injectable, self-setting pastes; (c) be naturally osteo-inductive and inhibitory for osteoclastogenesis; (d) intracellularly deliver bioactive compounds; (e) accommodate an array of functional ions; (f) be processed into macroporous constructs for tissue engineering; and (g) be naturally antimicrobial. All in all, we see in calcium phosphates the presence of a protean nature whose therapeutic potentials have been barely tapped into.

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“…Inorganic antibacterial agents possess advantages such as stability and safety. The antibacterial agents include ions such as copper, silver, and zinc [ 7 , 8 , 9 ], and several studies have shown that they can play important roles in the prevention or minimization of initial bacterial adhesion [ 10 , 11 ]. Metal ions can react with microbial membrane, causing structural changes and permeability.…”

Section: Introductionmentioning

confidence: 99%

Quenching of the Eu3+ Luminescence by Cu2+ Ions in the Nanosized Hydroxyapatite Designed for Future Bio-Detection

et al. 2021

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The hydroxyapatite nanopowders of the Eu3+-doped, Cu2+-doped, and Eu3+/Cu2+-co-doped Ca10(PO4)6(OH)2 were prepared by a microwave-assisted hydrothermal method. The structural and morphological properties of the products were investigated by X-ray powder diffraction (XRD), transmission electron microscopy techniques (TEM), and infrared spectroscopy (FT-IR). The average crystal size and the unit cell parameters were calculated by a Rietveld refinement tool. The absorption, emission excitation, emission, and luminescence decay time were recorded and studied in detail. The 5D0 → 7F2 transition is the most intense transition. The Eu3+ ions occupied two independent crystallographic sites in these materials exhibited in emission spectra: one Ca(1) site with C3 symmetry and one Ca(2) sites with Cs symmetry. The Eu3+ emission is strongly quenched by Cu2+ ions, and the luminescence decay time is much shorter in the case of Eu3+/Cu2+ co-doped materials than in Eu3+-doped materials. The luminescence quenching mechanism as well as the schematic energy level diagram showing the Eu3+ emission quenching mechanism using Cu2+ ions are proposed. The electron paramagnetic resonance (EPR) technique revealed the existence of at least two different coordination environments for copper(II) ion.

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Multifunctional lanthanide and silver ion co-doped nano-chlorapatites with combined spectroscopic and antimicrobial properties

Cited by 16 publications

References 24 publications

Multifunctionality of Nanosized Calcium Apatite Dual-Doped with Li+/Eu3+ Ions Related to Cell Culture Studies and Cytotoxicity Evaluation In Vitro

Multifunctionality of Nanosized Calcium Apatite Dual-Doped with Li+/Eu3+ Ions Related to Cell Culture Studies and Cytotoxicity Evaluation In Vitro

Calcium Phosphate as a Key Material for Socially Responsible Tissue Engineering

Quenching of the Eu3+ Luminescence by Cu2+ Ions in the Nanosized Hydroxyapatite Designed for Future Bio-Detection

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