Multifunctional sponge scaffold loaded with concentrated growth factors for promoting wound healing

Chen, Lianglong; Huang, Chaoyang; Yu, Zhongbo; Chen, Yujia; Zhang, Huihui; Zheng, Zijun; Jiang, Ziwei; Wei, Xuerong; Peng, Yujie; Huang, Lei; Niu, Li-Bin; Gao, Yanbin; Ma, Jun; Yang, Lei

doi:10.1016/j.isci.2022.105835

Cited by 10 publications

(3 citation statements)

References 59 publications

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“…Of course, a more detailed cell morphological and proteomic analysis is necessary to fully understand the PDA positive effect on cell growth observed in this kind of printable hydrogel. In addition, it may be interesting to evaluate whether a different percentage of PDA or a different quantity of PDA nanoparticles present in a hydrogel [34] could modulate the growth of the different cell population, such as endothelial cells or osteoblasts, to be able, for example, to create a PDA gradient that can act as an "organizer" of small models of complex organs.…”

Section: Discussionmentioning

confidence: 99%

Polydopamine Blending Increases Human Cell Proliferation in Gelatin–Xanthan Gum 3D-Printed Hydrogel

Yerra,

Migliario,

Gino

et al. 2024

Gels

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Background: Gelatin–xanthan gum (Gel–Xnt) hydrogel has been previously modified to improve its printability; now, to increase its ability for use as cell-laden 3D scaffolds (bioink), polydopamine (PDA), a biocompatible, antibacterial, adhesive, and antioxidant mussel-inspired biopolymer, has been added (1–3% v/v) to hydrogel. Methods: Control (CT) and PDA-blended hydrogels were used to print 1 cm2 grids. The hydrogels’ printability, moisture, swelling, hydrolysis, and porosity were tested after glutaraldehyde (GTA) crosslinking, while biocompatibility was tested using primary human-derived skin fibroblasts and spontaneously immortalized human keratinocytes (HaCaT). Keratinocyte or fibroblast suspension (100 µL, 2.5 × 105 cells) was combined with an uncrosslinked CT and PDA blended hydrogel to fabricate cylinders (0.5 cm high, 1 cm wide). These cylinders were then cross-linked and incubated for 1, 3, 7, 14, and 21 days. The presence of cells within various hydrogels was assessed using optical microscopy. Results and discussion: PDA blending did not modify the hydrogel printability or physiochemical characteristics, suggesting that PDA did not interfere with GTA crosslinking. On the other hand, PDA presence strongly accelerated and increased both fibroblast and keratinocyte growth inside. This effect seemed to be linked to the adhesive abilities of PDA, which improve cell adhesion and, in turn, proliferation. Conclusions: The simple PDA blending method described could help in obtaining a new bioink for the development of innovative 3D-printed wound dressings.

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Section: Discussionmentioning

confidence: 99%

Polydopamine Blending Increases Human Cell Proliferation in Gelatin–Xanthan Gum 3D-Printed Hydrogel

Yerra,

Migliario,

Gino

et al. 2024

Gels

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“…Other biomaterials enriched with PL and PRF for STE applications are presented in Table 3. In turn, Chen et al [134] fabricated a skin substitute composed of an acellular dermal matrix (ADM) cross-linked by glutaraldehyde with chitosan, modified with heparin, dopped with polydopamine nanospheres (concentration 2 g/L) and enriched with CFG (marked as CADMS-G-2-Hep-CGF). It was demonstrated that this biomaterial promoted the migration and proliferation of mouse fibroblasts and human endothelial cells in vitro.…”

Section: Wound Dressings and Skin Substitutes Enriched With Pcs Other...mentioning

confidence: 99%

Recent Achievements in the Development of Biomaterials Improved with Platelet Concentrates for Soft and Hard Tissue Engineering Applications

Grzelak,

Hnydka,

Higuchi

et al. 2024

IJMS

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Platelet concentrates such as platelet-rich plasma, platelet-rich fibrin or concentrated growth factors are cost-effective autologous preparations containing various growth factors, including platelet-derived growth factor, transforming growth factor β, insulin-like growth factor 1 and vascular endothelial growth factor. For this reason, they are often used in regenerative medicine to treat wounds, nerve damage as well as cartilage and bone defects. Unfortunately, after administration, these preparations release growth factors very quickly, which lose their activity rapidly. As a consequence, this results in the need to repeat the therapy, which is associated with additional pain and discomfort for the patient. Recent research shows that combining platelet concentrates with biomaterials overcomes this problem because growth factors are released in a more sustainable manner. Moreover, this concept fits into the latest trends in tissue engineering, which include biomaterials, bioactive factors and cells. Therefore, this review presents the latest literature reports on the properties of biomaterials enriched with platelet concentrates for applications in skin, nerve, cartilage and bone tissue engineering.

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“…On one hand, due to their ability in retaining fluids, they are useful in absorbing exudates from the wound, thus creating a conducive environment for cell proliferation and migration[ 201 ]. On the other hand, they can be embedded with media enriched with growth factors that can be released once implanted in the wound bed and effectively promote skin regeneration and wound healing[ 212 ]. Due to their caveolar structure, sponge-like scaffolds (SLS) are exceptionally suitable for hosting MSC cells, whose adhesion and numerosity can be augmented in functionalized SLS ( i.e.…”

Section: Diabetes Comorbiditiesmentioning

confidence: 99%

Alternative therapeutic strategies in diabetes management

Annicchiarico,

Barile,

Buccoliero

et al. 2024

World J Diabetes

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Diabetes is a heterogeneous metabolic disease characterized by elevated blood glucose levels resulting from the destruction or malfunction of pancreatic β cells, insulin resistance in peripheral tissues, or both, and results in a non-sufficient production of insulin. To adjust blood glucose levels, diabetic patients need exogenous insulin administration together with medical nutrition therapy and physical activity. With the aim of improving insulin availability in diabetic patients as well as ameliorating diabetes comorbidities, different strategies have been investigated. The first approaches included enhancing endogenous β cell activity or transplanting new islets. The protocol for this kind of intervention has recently been optimized, leading to standardized procedures. It is indicated for diabetic patients with severe hypoglycemia, complicated by impaired hypoglycemia awareness or exacerbated glycemic lability. Transplantation has been associated with improvement in all comorbidities associated with diabetes, quality of life, and survival. However, different trials are ongoing to further improve the beneficial effects of transplantation. Furthermore, to overcome some limitations associated with the availability of islets/pancreas, alternative therapeutic strategies are under evaluation, such as the use of mesenchymal stem cells (MSCs) or induced pluripotent stem cells for transplantation. The cotransplantation of MSCs with islets has been successful, thus providing protection against proinflammatory cytokines and hypoxia through different mechanisms, including exosome release. The use of induced pluripotent stem cells is recent and requires further investigation. The advantages of MSC implantation have also included the improvement of diabetes-related comorbidities, such as wound healing. Despite the number of advantages of the direct injection of MSCs, new strategies involving biomaterials and scaffolds have been developed to improve the efficacy of mesenchymal cell delivery with promising results. In conclusion, this paper offered an overview of new alternative strategies for diabetes management while highlighting some limitations that will need to be overcome by future approaches.

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Multifunctional sponge scaffold loaded with concentrated growth factors for promoting wound healing

Cited by 10 publications

References 59 publications

Polydopamine Blending Increases Human Cell Proliferation in Gelatin–Xanthan Gum 3D-Printed Hydrogel

Polydopamine Blending Increases Human Cell Proliferation in Gelatin–Xanthan Gum 3D-Printed Hydrogel

Recent Achievements in the Development of Biomaterials Improved with Platelet Concentrates for Soft and Hard Tissue Engineering Applications

Alternative therapeutic strategies in diabetes management

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