Multifunctional ultrasmall AgNP hydrogel accelerates healing of S. aureus infected wounds

Haidari, Hanif; Bright, Richard; Strudwick, Xanthe L.; Garg, Sanjay; Vasilev, Krasimir; Cowin, Allison J.; Kopecki, Zlatko

doi:10.1016/j.actbio.2021.04.007

Cited by 95 publications

(81 citation statements)

References 65 publications

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“…The total photon emission from defined wound areas within the images, region of interest (ROI) was quantified with Living Image R software following established protocols [36]. At two-day post-inoculation, once the bacterial infection was well established, and the presence of mature biofilms was evident by the formation of extracellular polymeric substance (EPS), the wounds were treated daily with either 60 µL of AgNP hydrogel (200 µg/g) equivalent to (12 µg of silver) per treatment, blank hydrogel, and 1% Ag SD cream equivalent to (302 µg of silver) until day 9 and imaged daily to monitor the bacterial burden [24]. Wounds were covered with a sterile Tegaderm TM dressing daily from day 0 to day 10 and post each treatment to create a fertile environment for the development of mature bacterial biofilms and mimic the clinical environment [37].…”

Section: In Vivo Mature Biofilm Wound Infection Animal Model and Treatmentmentioning

confidence: 99%

“…The wound tissue was halved through the center and half of the tissue was submitted for fixation and subsequent hematoxylin and eosin (H&E) tissue staining for histological analysis of wound healing. The remaining half of the wound was bisected, a quarter was used for bacterial colony counts, and a quarter for live/dead bacterial viability staining [24]. Collected organs were weighed and processed for H&E staining.…”

Section: In Vivo Mature Biofilm Wound Infection Animal Model and Treatmentmentioning

confidence: 99%

“…Our previous study demonstrated the potential of a thermoresponsive ultrasmall AgNP hydrogel as an antibacterial agent against acute wound infection (resembling planktonic bacterial growth) [24]. Most acute wound infections are transient and resolve spontaneously within 12 weeks depending on host immune responses [25].…”

Section: Introductionmentioning

confidence: 99%

“…However, chronic infections observed in non-healing wounds are usually associated with persistent bacterial colonization accompanied with delayed healing, which often requires long-term antibacterial therapy at a much higher concentration [26]. Despite the benefits of current antibacterial hydrogels to control short-term acute infection [24], the potential of these approaches has not been demonstrated in complex models of mature biofilm wound infection. These biofilm models feature higher levels of pathogenic bacteria-a larger wound size has a high degree of exudate and maceration and they utilize the application of a wound dressing, which not only closely mimics the clinical situation but also promotes mature biofilm development.…”

Section: Introductionmentioning

confidence: 99%

“…One strategy to overcome these limitations is the fabrication of a controlled release system containing a stable and optimized concentration of ultrasmall AgNPs, harnessing high bactericidal effects with significantly lower or absent cytotoxicity. This tailored approach has been shown to be effective against planktonic bacteria [24]; however, its ability to overcome resistant mature biofilms in chronic wounds has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

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Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

et al. 2021

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Biofilm-associated infections are a major cause of impaired wound healing. Despite the broad spectrum of anti-bacterial benefits provided by silver nanoparticles (AgNPs), these materials still cause controversy due to cytotoxicity and a lack of efficacy against mature biofilms. Herein, highly potent ultrasmall AgNPs were combined with a biocompatible hydrogel with integrated synergistic functionalities to facilitate elimination of clinically relevant mature biofilms in-vivo combined with improved wound healing capacity. The delivery platform showed a superior release mechanism, reflected by high biocompatibility, hemocompatibility, and extended antibacterial efficacy. In vivo studies using the S. aureus wound biofilm model showed that the AgNP hydrogel (200 µg/g) was highly effective in eliminating biofilm infection and promoting wound repair compared to the controls, including silver sulfadiazine (Ag SD). Treatment of infected wounds with the AgNP hydrogel resulted in faster wound closure (46% closure compared to 20% for Ag SD) and accelerated wound re-epithelization (60% for AgNP), as well as improved early collagen deposition. The AgNP hydrogel did not show any toxicity to tissue and/or organs. These findings suggest that the developed AgNP hydrogel has the potential to be a safe wound treatment capable of eliminating infection and providing a safe yet effective strategy for the treatment of infected wounds.

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Section: In Vivo Mature Biofilm Wound Infection Animal Model and Treatmentmentioning

confidence: 99%

Section: In Vivo Mature Biofilm Wound Infection Animal Model and Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

et al. 2021

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Recent Advances in Poly(N‐isopropylacrylamide) Hydrogels and Derivatives as Promising Materials for Biomedical and Engineering Emerging Applications

et al. 2023

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Temperature‐sensitive (thermosensitive) hydrogels, which are part of the family of stimulus‐sensitive hydrogels, consist of water‐filled polymer networks that display a temperature‐dependent degree of swelling. Thermosensitive hydrogels, which can undergo phase transition or swell/de‐swell as temperature changes, have great potential in various technological and biomedical purposes for a number of reasons: their temperature response is reversible, hydrogels are stable and easy to prepare, they can be biocompatible and also be suitably combined with other organic and inorganic materials, resulting in new materials with outstanding properties. Among thermosensitive hydrogels poly(N‐isopropylacrylamide) (PNIPAAm) is the most extensively studied because it brings together the best properties of these materials. Consequently, in the past few years, a wide number of applications and new chemical processes to prepare PNIPAAm and their derivatives are being proposed. The objective of this review is to summarize the fundamentals of thermosensitive hydrogels and recent advances in preparation and both technological and biomedical applications of thermosensitive hydrogel, with a special focus on PNIPAAm and their derivatives. Special attention has been given to the discussion of challenges and future research perspectives based on new horizons not yet considered.

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Advanced Biomaterials for Regulating Polarization of Macrophages in Wound Healing

Mao

Chen

Cai

et al. 2021

Adv Funct Materials

120

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Macrophage immunotherapy is an emerging treatment strategy that modulates the immune system to promote wound healing. The functionality and regeneration of tissue depend on spatially and temporally regulating the biophysical and biochemical microenvironmental with poorly understood mechanisms. Biomaterials are carefully crafted to display and deliver macrophage regulatory signals in a precise and near‐physiological way, serving as powerful artificial microenvironments in which to explore and direct the fate of macrophages. The review starts with discussing the classification and function of macrophages, and then introduces the polarization of macrophages in different microenvironments of conventional wounds and chronic wounds. Recent advances in biomaterials that balance the phenotypes of macrophages in wound healing are emphasized. Finally, looking ahead, the potential ability of biomaterial scaffolds to modulate immune signaling to produce an environment conducive to regeneration is discussed.

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Multifunctional ultrasmall AgNP hydrogel accelerates healing of S. aureus infected wounds

Cited by 95 publications

References 65 publications

Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

Recent Advances in Poly(N‐isopropylacrylamide) Hydrogels and Derivatives as Promising Materials for Biomedical and Engineering Emerging Applications

Advanced Biomaterials for Regulating Polarization of Macrophages in Wound Healing

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