Multigene phylogeny and morphology reveal that the Chinese medicinal mushroom ‘Cordyceps gunnii’ is Metacordyceps neogunnii sp. nov.

Wen, Ting‐Chi; Xiao, Yuan-Pin; Han, Yan-Feng; Huang, Shi-Ke; Zha, Ling-Sheng; Hyde, Kevin D.; Kang, Ji-Chuan

doi:10.11646/phytotaxa.302.1.2

Cited by 17 publications

(22 citation statements)

References 34 publications

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“…pseudoatrovirens TNSF-16380 Japan Coleoptera – JF415977 – JN049893 JF415997 JN049870 – Kepler et al (2012a) Mc. shibinensis GZUH SB13050311 China Lepidoptera KR153588 – KR153589 KR153590 – – – Wen et al (2015) Metacordyceps sp. ARSEF 2038 Korea Hemiptera : Delphacidae – – KJ398806 KJ398616 – – – Kepler et al (2014) GZUH SB13050305 China Lepidoptera larva KU729724 – KU729729 KU729734 – KY423507 – Wen et al (2017) SK 2014 India Hymenoptera – – – – – KJ179838 – Sharma & Gautam (2015) Mc.…”

Section: Methodsmentioning

confidence: 99%

“…Over the last decade, with increased availability of sequence data, recognition of new species in Metarhizium increased our understanding of its diversity, host affiliation, ecology and distribution with new species described from Argentina ( Gutierrez et al. 2019 ), Brazil ( Montalva et al., 2016 , Lopes et al., 2018 , Luz et al., 2019 ), China ( Li et al., 2010 , Chen et al., 2017 , Wen et al., 2017 , Chen et al., 2018a , Chen et al., 2018c ), Japan ( Nishi et al., 2017 , Iwasaki et al., 2019 , Yamamoto et al., 2020 ), and Thailand ( Luangsa-ard et al., 2017 , Thanakitpipattana et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Revisiting Metarhizium and the description of new species from Thailand

Mongkolsamrit¹,

Khonsanit²,

Thanakitpipattana³

et al. 2020

Studies in Mycology

100

101

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Over the last two decades the molecular phylogeny and classification of Metarhizium has been widely studied. Despite these efforts to understand this enigmatic genus, the basal lineages in Metarhizium are still poorly resolved. In this study, a phylogenetic framework is reconstructed for the Clavicipitaceae focusing on Metarhizium through increased taxon-sampling using five genomic loci (SSU, LSU, tef, rpb1, rpb 2) and the barcode marker ITS rDNA. Multi-gene phylogenetic analyses and morphological characterisation of green-spored entomopathogenic Metarhizium isolates from Thailand and soil isolates of M. carneum and M. marquandii reveal their ecological, genetic and species diversity. Nineteen new species are recognised in the Metarhizium clade with narrow host ranges: two new species are found in the M. anisopliae complex – M. clavatum on Coleoptera larvae and M. sulphureum on Lepidoptera larvae; four new species are found in the M. flavoviride complex – M. biotecense and M. fusoideum on brown plant hoppers ( Hemiptera ), M. culicidarum on mosquitoes, M. nornnoi on Lepidoptera larvae; three new species M. megapomponiae, M. cicadae, M. niveum occur on cicadas; five new species M. candelabrum, M. cercopidarum, M. ellipsoideum, M. huainamdangense M. ovoidosporum occur on planthoppers, leafhoppers and froghoppers ( Hemiptera ); one new species M. eburneum on Lepidoptera pupae; and four new species M. phuwiangense, M. purpureum, M. purpureonigrum, M. flavum on Coleoptera . Of these 19 new species, seven produce a sexual morph ( M. clavatum, M. eburneum, M. flavum, M. phuwiangense, M. purpureonigrum, M. purpureum, and M. sulphureum ) and asexual morphs are found in the remaining new species and also in M. sulphureum, M. purpureonigrum and M. purpureum. Metarhizium blattodeae, M. koreanum and M. viridulum are new records for Thailand. An alternative neotype for Metarhizium anisopliae is proposed based on multi-gene and 5′ tef analyses showing that CBS 130.71 from Ukraine is more suitable, being from a much closer geographical location to Metchnikoff’s Metarhizium anisopliae. This isolate is distinct from the neotype of Metarhizium anisopliae var. anisopl...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Revisiting Metarhizium and the description of new species from Thailand

Mongkolsamrit¹,

Khonsanit²,

Thanakitpipattana³

et al. 2020

Studies in Mycology

100

101

View full text Add to dashboard Cite

show abstract

“…Berk. early reported in China which is widely present in Guizhou, Hunan and Anhui provinces [1], has various effects such as analgesia, sedation, improvement of human immunity, anti-tumor, anti-aging, promoting sleep and enhancing memory [2][3][4][5]. It has chemical composition and medicinal value similar to those of Cordyceps sinensis.…”

Section: Introductionmentioning

confidence: 99%

Identification and Analysis of Secondary Metabolites from CH₃OH/H₂O Extract of Metacordyceps Neogunnii

et al. 2019

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Secondary metabolites are considered to be the major compounds in Cordyceps with anti-tumor, anti-aging and immunity-enhancing effects. The molecular structures of secondary metabolites form the basis for the development and utilization of Cordyceps. Metacordyceps neogunnii is an important Cordyceps resource, but less study has been made on the molecular structure of its secondary metabolites. In this study, gas chromatography-mass spectrometry (GC/MS) and ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) were used to analyze and identify the secondary metabolites from CH3OH/H2O extract of Metacordyceps neogunnii. The results show that a total of 22 compounds were identified by GC-MS, including 2 n-alkanes, 1 isoparaffin, 1 cycloalkane, 6 olefins and 13 esters. The predominant compounds were (E)-ocimene, (E)-β-ocimene, methyl oleate, dioctyl adipate, methyl palmitate and methyl linoleate, obtained by means of GC/MS. Five distince classes of secondary metabolites were speculated: 3 polypeptides, 2 esters, 1 isoflavone, 1 isoindrone and 3 amides, from which 10 compounds were detected using UPLC-Q-TOF/MS. (R)-N-((1-(((9H-fluorene-9-yl) methoxy) carbonyl) pyrrolidine-2-yl) methyl)-N-(2-(6-benzoylamino-9H-purin-9-yl) acetyl) glycine, benzyl (5-(2-((3-(2, 3-dihydrobenzo[b] [1, 4] dioxin-6-yl)-4-carbonyl-4H-chromene-7-yl) oxo) acetylamino) amyl) carbamate, 5, 5’-((propane-2, 2-diyl (4, 1-phenylene)) bis (oxo)) bis (2-(naphthalene-1-yl) isoindoline-1, 3-dione), 1-dodecylazepine-2-one and other compounds were the first detected in Metacordyceps neogunnii.

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“…This group currently has more than 900 species that belong to three families (Cordycipitaceae, Ophiocordycipitaceae and partial Clavicipitaceae) in the order Hypocreales (Zha et al 2018). More than 140 species have been reported in China (Wen et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Taxonomy and biology of Cordyceps qingchengensis sp. nov. and its allies

et al. 2019

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Cordyceps qingchengensis sp. nov., growing on a cocooned pupa of a silk moth (Lepidoptera: Bombycidae) and collected from southwestern China, is described, illustrated and compared with allied taxa. The species is morphologically similar to C. bifusispora and C. tenuipes, but can be easily separated from the latter two by the unique host and by branched and thicker stroma. Phylogenetic analyses of single ITS and combined SSU, LSU and TEF1-α datasets indicate that it is closely related to C. bifusispora, C. cicadae (Miq.) Massee (Chanhua) and C. tenuipes, but C. qingchengensis has distinct nucleotide differences which support it as new. Taxonomy of C. tenuipes and C. pruinosa is reviewed and C. ninchukispora (≡ Phytocordyceps ninchukispora) is considered as a synonym of C. pruinosa. Ecology and life cycles of C. qingchengensis, C. tenuipes, C. pruinosa and C. ningxiaensis are recorded and inferred. We provide important biological information for C. qingchengensis and its allies.

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Multigene phylogeny and morphology reveal that the Chinese medicinal mushroom ‘Cordyceps gunnii’ is Metacordyceps neogunnii sp. nov.

Cited by 17 publications

References 34 publications

Revisiting Metarhizium and the description of new species from Thailand

Revisiting Metarhizium and the description of new species from Thailand

Identification and Analysis of Secondary Metabolites from CH₃OH/H₂O Extract of Metacordyceps Neogunnii

<p><strong>Taxonomy and biology of <em>Cordyceps qingchengensis sp. nov</em>. and its allies</strong></p>

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Multigene phylogeny and morphology reveal that the Chinese medicinal mushroom ‘Cordyceps gunnii’ is Metacordyceps neogunnii sp. nov.

Cited by 17 publications

References 34 publications

Revisiting Metarhizium and the description of new species from Thailand

Revisiting Metarhizium and the description of new species from Thailand

Identification and Analysis of Secondary Metabolites from CH3OH/H2O Extract of Metacordyceps Neogunnii

<p><strong>Taxonomy and biology of <em>Cordyceps qingchengensis sp. nov</em>. and its allies</strong></p>

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Identification and Analysis of Secondary Metabolites from CH₃OH/H₂O Extract of Metacordyceps Neogunnii