Multilayer Electrodes DEP Chip for Single-cell Level Impedance Measurement

Chuang, Cheng-Hsin; Wei, Ching-Hua; Hsu, Yi-Chu; Lu, Jian-Tang

doi:10.1109/nems.2007.352144

Cited by 5 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the device does not need an extra AC signal for particle trapping. The impedance measurement results reported by Chuang et al [10] show that the impedance magnitude of a single 16 µm particle is higher than that of the PBS, which is consistent with the results in the present work. However, the impedance variation in this work is much higher, making it much easier to distinguish the impedance of a particle from that of PBS solution.…”

Section: Impedance Measurement Of Single Particlesupporting

confidence: 93%

“…Chuang et al [10] presented a 3D microcavity structure for trapping particles and impedance measurement. However, an extra AC signal, which may influence measurement results, is required for the dielectrophoresis (DEP) force to move and trap a single particle in the microcavity.…”

Section: Impedance Measurement Of Single Particlementioning

confidence: 99%

“…However, even when the cell makes contact with the electrodes, only a few parts of the cell are located in the strong electric field. Compared to planar electrodes, three-dimensional (3D) electrode geometries and multilayer electrode geometries provide a much more uniform electric field distribution [8][9][10]. Therefore, most parts of a cell are exposed to the high and uniform electric field, possibly increasing the sensitivity of impedance measurement.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Particle trapping and impedance measurement using bilayer electrodes integrated with microcavity structure

Chen

Liu

Jang

et al. 2017

J. Micromech. Microeng.

View full text Add to dashboard Cite

Traditional planar electrodes for single-particle impedance measurement have difficulty in trapping and positioning particles. This paper proposes a microfluidic device for single-particle trapping and impedance measurement with a microcavity configuration. A carbon dioxide (CO2) laser technique was used to fabricate the microcavity structure, which can capture 15 µm diameter particles without requiring additional trapping structures. The measurement electrodes on both sides of the microcavity were fabricated using electroplating and deposition techniques. The advantages of the microcavity structure and electrodes are discussed. The bottom electrode spreads into the microcavity to increase measurement sensitivity and shrink the exit aperture to around 10 µm for particle trapping. The experimental results show that the device successfully captured particles and distinguished the impedance of a particle from that of phosphate-buffered saline solution.

show abstract

Section: Impedance Measurement Of Single Particlesupporting

confidence: 93%

Section: Impedance Measurement Of Single Particlementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Particle trapping and impedance measurement using bilayer electrodes integrated with microcavity structure

Chen

Liu

Jang

et al. 2017

J. Micromech. Microeng.

View full text Add to dashboard Cite

show abstract

“…Subsequently, Suehiro 71 , et al proposed a similar structure where the electrode system consisted of two glass plates, on which parallel strip electrodes were fabricated, placed together with a spacer between them so that their electrodes face each other and cross at right angles to form the grid. Such structures have also been reported by many groups [72][73][74][75][76][77][78] . Recently, a novel system with thin film vertical electrodes deposited on both sides of the microchannel for DEP trapping has been reported by Wang 79 , et al The vertical metal electrodes with different length ratios were fabricated by electroplating on the SU-8 microchannel to generate the non-uniformity of the electric field for particle manipulation.…”

Section: Step(d)supporting

confidence: 78%

Particle Manipulation by Miniaturised Dielectrophoretic Devices

Tay

Iliescu

2009

DSJ

View full text Add to dashboard Cite

This paper presents a review of dielectrophoresis (DEP) devices which provide an effective way to manipulate and separate micro-or nano-bioparticles automatically and quickly by polarisation effects in a nonuniform electric field. A detailed review for designs and operation principles of various microfabricated DEP devicesis given and some advantages and disadvantages of current devices are noted to the final system to attain the unprecedented levels of performance.

show abstract

“…In 2007, Zhang et al [ 5 ] proposed a different design for impedance measurement with single-cell resolution; he separated the electrodes for individual purposes of DEP trapping and impedance measurement; thus, long-term monitoring of dynamic process of endothelin-1-induced cardiomyocyte hypertrophy could be achieved. In the meanwhile, Chuang [ 6 , 7 ] demonstrated a DEP chip with multilayer electrodes and a micro-cavity array for trapping single cells in a micro-cavity by negative DEP force and sequentially sensing their impedance. In Chuang’s work, the impedance measurement of trapped cells in a micro-cavity did not have to apply DEP force due to the enhancement of the positioning and immobilization by the microstructure effects.…”

Section: Introductionmentioning

confidence: 99%

System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells

Chuang

Huang

2011

Sensors

Self Cite

View full text Add to dashboard Cite

This paper develops a dielectrophoretic (DEP) chip with multi-layer electrodes and a micro-cavity array for programmable manipulations of cells and impedance measurement. The DEP chip consists of an ITO top electrode, flow chamber, middle electrode on an SU-8 surface, micro-cavity arrays of SU-8 and distributed electrodes at the bottom of the micro-cavity. Impedance sensing of single cells could be performed as follows: firstly, cells were trapped in a micro-cavity array by negative DEP force provided by top and middle electrodes; then, the impedance measurement for discrimination of different stage of bladder cancer cells was accomplished by the middle and bottom electrodes. After impedance sensing, the individual releasing of trapped cells was achieved by negative DEP force using the top and bottom electrodes in order to collect the identified cells once more. Both cell manipulations and impedance measurement had been integrated within a system controlled by a PC-based LabVIEW program. In the experiments, two different stages of bladder cancer cell lines (grade III: T24 and grade II: TSGH8301) were utilized for the demonstration of programmable manipulation and impedance sensing; as the results show, the lower-grade bladder cancer cells (TSGH8301) possess higher impedance than the higher-grade ones (T24). In general, the multi-step manipulations of cells can be easily programmed by controlling the electrical signal in our design, which provides an excellent platform technology for lab-on-a-chip (LOC) or a micro-total-analysis-system (Micro TAS).

show abstract

Multilayer Electrodes DEP Chip for Single-cell Level Impedance Measurement

Cited by 5 publications

References 4 publications

Particle trapping and impedance measurement using bilayer electrodes integrated with microcavity structure

Particle trapping and impedance measurement using bilayer electrodes integrated with microcavity structure

Particle Manipulation by Miniaturised Dielectrophoretic Devices

System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells

Contact Info

Product

Resources

About