Multilayer film elements for clinical analysis: applications to representative chemical determinations.

Spayd, R W; Bruschi, B; Burdick, Bruce A.; Dappen, G M; Eikenberry, Jon N.; Esders, Theodore W.; Figueras, John; Goodhue, C. T.; LaRossa, D D; Nelson, Randall W.; Rand, R N; Wu, T W

doi:10.1093/clinchem/24.8.1343

Cited by 324 publications

(70 citation statements)

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“…We measured the total serum cholesterol concentration using the endpoint colorimetry method of Allain and colleagues (18) and a Vitros 950 analyzer (Ortho Diagnostics, Rochester, New York, USA), and the serum HDLcholesterol concentration using Kodak Vitros cholesterol slides and a Vitros 250 analyzer (Ortho Diagnostics) (19). Serum triacylglycerol concentration was determined by the method of Spayd and coworkers (20) with a Vitros analyzer clinical chemistry slide and a Vitros 950 analyzer (Ortho Diagnostics). In order to calculate serum LDL-cholesterol concentrations, we used the equation LDL-cholesterol ¼ total cholesterol À (HDL-cholesterol þ Triaclyglycerol/5).…”

Section: Biochemical Analyzesmentioning

confidence: 99%

High-density lipoprotein and homocysteine levels correlate inversely in preeclamptic women in northern Nigeria

VanderJagt

Patel

El-Nafaty

et al. 2004

Acta Obstet Gynecol Scand

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Background. Preeclampsia/eclampsia is one of the most common complications of pregnancy. It is a cause of high morbidity for both mother and fetus, especially in developing countries. In a recent survey conducted in Gombe, Nigeria, eclampsia was found to be a major cause of maternal mortality (24.2%), second only to obstetric hemorrhage (27.1%). Previous studies have produced contradictory findings regarding total homocysteine (tHcy) levels in women with preeclampsia/eclampsia and there is little information about the relationship between particular serum lipids and tHcy. The objective of this study in Gombe was to compare the levels of serum lipids and homocysteine in healthy pregnant women and women with preeclampsia/eclampsia in Nigeria. Methods. The experimental subjects included 43 women with preeclampsia/eclampsia and 130 healthy pregnant women served as controls. The criteria for preeclampsia/eclampsia included the following: hypertension (blood pressure > 140/90 mmHg), total protein (> 190 mg/g creatinine), and edema. Blood sera obtained from patients and controls attending the prenatal clinics at the Specialist Hospital and the Federal Medical Center in Gombe were analyzed for tHcy, total cholesterol, triglycerides, high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol, antioxidant capacity, folate, and vitamin B-12. Results. The mean tHcy concentration for the preeclamptic/eclamptic women was greater than that of the controls (10.1 vs. 8.4 mmol/l, respectively, p ¼ 0.01). The mean concentrations of LDL-cholesterol and triacylglycerols were not different between the two groups. However, the mean HDL-cholesterol level was higher in the healthy pregnant women compared with the preeclamptic/eclamptic women (1.64 vs. 1.42 mmol/l, respectively, p ¼ 0.02). The HDL-cholesterol concentration was correlated inversely with the tHcy concentration (p ¼ 0.001, r ¼ 0.51). Total homocysteine was not linked with either serum folate or vitamin B-12. Conclusions. These results show that preeclampsia/eclampsia is associated with increased tHcy levels and that HDL levels are depressed in Nigerian women with this hypertensive, pregnancy associated disorder.

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Section: Biochemical Analyzesmentioning

confidence: 99%

High-density lipoprotein and homocysteine levels correlate inversely in preeclamptic women in northern Nigeria

VanderJagt

Patel

El-Nafaty

et al. 2004

Acta Obstet Gynecol Scand

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“…Triacylglycerol synthesis in HTC cells cultured in serum-free α-MEM medium containing oleate and insulin or no insulin was determined by the enzymatic method (22). Triacylglycerol secretion in HTC cells was stimulated by insulin as a stimulator of hepatic lipogenesis in a dose-dependent manner, but was not observed in these cells cultured in the absence of insulin (Fig.…”

Section: Urea and Triacylglycerol Synthesis In Htc Cellsmentioning

confidence: 99%

Development of hepatocytes from embryonic stem cells after transfection with the HNF‐3β gene

Ishizaka¹,

Shiroi²,

Kanda³

et al. 2002

FASEB j.

111

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We have attempted to generate embryonic stem (ES) cell-derived hepatocytes expressing liver-specific functional properties by use of ES cell technology. It was found that ES cells are allowed to differentiate into hepatocytes possessing high metabolic activities when hepatocyte nuclear factor (HNF)-3beta-transfected ES cells are cultured in alpha-MEM medium supplemented with 10% fetal bovine serum (FBS) and fibroblast growth factor (FGF)-2 in the three-dimensional cell culture system at 5% CO2. The differentiated cells induced albumin, triacylglycerol, urea, and glycogen synthesis as well as further expression of metabolic proteins and serum factors as markers of hepatocytic differentiation for at least 4 months. The cells differentiated from HNF-3beta-transfected ES cells also had hepatocyte-like ultrastructural characteristics, including several endoplasmic reticula, mitochondrion, and glycogen. Our findings indicate that generation of hepatocytes maintaining high metabolic functions developed from mouse ES cells will facilitate the study of the basic mechanism for hepatogenesis and will certainly provide new opportunities for tissue transplantation.

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“…Total cholesterol, triacylglycerol and phospholipid concentrations in plasma samples and liver homogenates were assayed by the enzymatic methods of Allain et al (1974), Takayama et al (1977) and Spayd et al (1978) using Cholesterol E-test Wako, Triglyceride E-test Wako and Phospholipid C-test Wako (Wako Pure Chemical Industries, Ltd., Osaka, Japan), respectively. Plasma glucose concentrations were measured by the method of Trinder (1969) using an oxidase-peroxidase system with Glucose CII-test Wako (Wako Pure Chemical Industries, Ltd.).…”

Section: Lipid Glucose and Fatty Acid Analysesmentioning

confidence: 99%

CHANGES IN LEVELS OF PLASMA TOTAL CHOLESTEROL, TRIACYLGLYCEROL AND PHOSPHOLIPID IN MICE FED ERABU SEA SNAKE (LATICAUDA SEMIFASCIATA) LIPIDS DIET

Higuchi

Shirai

Suzuki

et al. 2008

Journal of Food Lipids

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The effects of dietary Erabu sea snake lipids on changes in plasma total cholesterol, triacylglycerol, and phospholipid levels of male Crlj : CD‐1 (ICR) mice was evaluated. Mice were fed three different experimental diets containing 6% lard, 6% Erabu sea snake lipid and 6% fish oil, respectively. Total cholesterol, triacylglycerol and phospholipid levels in plasma collected at weeks 0, 4, 8, 12 and 16 were measured. Levels of plasma total cholesterol and phospholipids in the sea snake dietary group were significantly reduced at weeks 8‐16 from the start of the trial, and plasma triacylglycerol was significantly reduced at week 16, while those in the lard dietary group remained unchanged and/or increased during the period of feeding trial. Plasma total cholesterol and triacylglycerol levels were significantly lower in sea snake lipids as well as fish oil dietary groups than in the lard dietary group at weeks 4–16. The results suggest that Erabu sea snake lipids rapidly induce reduction of plasma lipid levels at week 4 and reduction of plasma lipids and glucose in mice fed the sea snake lipids diet was influenced by the presence of n‐3 polyunsaturated fatty acids in the feed. PRACTICAL APPLICATIONS Erabu sea snake lipids have large amounts of n‐3 polyunsaturated fatty acids, and a lowering‐effect on levels of plasma and hepatic total cholesterol, triacylglycerol, phospholipid and plasma glucose in mice similar to fish oil. The sea snake lipids may serve as a beneficial food with regard to lipid and glucose metabolism.

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Multilayer film elements for clinical analysis: applications to representative chemical determinations.

Cited by 324 publications

References 0 publications

High-density lipoprotein and homocysteine levels correlate inversely in preeclamptic women in northern Nigeria

High-density lipoprotein and homocysteine levels correlate inversely in preeclamptic women in northern Nigeria

Development of hepatocytes from embryonic stem cells after transfection with the HNF‐3β gene

CHANGES IN LEVELS OF PLASMA TOTAL CHOLESTEROL, TRIACYLGLYCEROL AND PHOSPHOLIPID IN MICE FED ERABU SEA SNAKE (LATICAUDA SEMIFASCIATA) LIPIDS DIET

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