Multilayer films containing immobilized nucleic acids. Their structure and possibilities in biosensor applications

Sukhorukov, Gleb B.; Montrel, M.M.; Петров, А. И.; Шабарчина, Л. И.; Сухоруков, Б. И.

doi:10.1016/0956-5663(96)89440-1

Cited by 122 publications

(86 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…31). Wet chemistry cross-linking by aldehydes has been realized 253,352,358,366) , as well as reduction of graphite oxide by hydrazine and hydrogen 324) , chemical ring closure of polyimides 164,387) , azo coupling 142,148,214) , and the intercalation of acridin orange and related dyes into DNA 20,[333][334][335]343) . Also, much interest has been given to the conversion of substrates by enzymes in ESA-films 248,256,340,[346][347][348][349] .…”

Section: Chemical Reactions In Films Made By Esamentioning

confidence: 99%

“…Ribonucleic and desoxyribonucleic acids (RNA and DNA) are strong polyanions due to the phosphate esters in their backbone and thus deposit easily, as do the model homopolynucleotides 24,26,36,38,116,317,[331][332][333][334][335][336][337][338][339][340][341][342][343] . Whereas intercalation studies have been successful [333][334][335]341) , molecular recognition and selective pairing of single strands is difficult to accomplish yet in ESA-films 26,116,333,338) . Also, a number of mixed protein-polyelectrolyte or protein-protein films 38,108,109,155,202,209,237,248,253,256,265,274,277,279,317,318,[344][345][346][347]…”

Section: Polymers Suited For Esamentioning

confidence: 99%

See 1 more Smart Citation

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,173

1,184

View full text Add to dashboard Cite

A bit of historyThe key role of surfaces for many material properties as well as for many biological processes has been recognized now. Therefore, new strategies aim at the tailoring of the material's surface only -or of a thin surface layer, respectively -, while preserving the bulk properties of the underlying support. Particular emphasis has been given to the surface modification by polymers, in an attempt to extend the known versatility of polymer bulk materials to ultrathin films and coatings, and to prepare bulk-surface composite materials. The self-organization of polymers has been increasingly explored for the preparation of well-defined surfaces and interfaces in recent years, extending the use of the established methods of low molar mass compounds (for comparative reviews, see ref. 1-3)). With such techniques, polymer films are formed spontaneously on substrates, due to balanced interactions between substrate, polymer (or its precursor) and medium. Typically, very thin, often monomolecular layers are produced. Repetitive deposition steps provide a precise control over the total thickness of the coatings, in the range from a few angstroms up to the micrometer range. Moreover, the step-by-step procedures allow for a fine structuring in the third dimension. In addition to the preparation of uniform and homogeneous coatings, gratings, gradients or steps of defined height in molecular dimensions can be easily constructed.The most recent of the self-organization techniques is the alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA) [3][4][5][6][7][8][9][10] . Although some early, singular studies of self-assembly by alternating adsorption of oppositely charged polyions are reported [11][12][13] , a practical method for ESA was developed Feature Article: The article presents the state-of-the-art of alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA), for the preparation of thin polymer coatings. In comparison to other, more established self-organization techniques, this recent method is distinguished by its simplicity, versatility, and speed. In particular, the tendency for self-healing is unique. Emphasis is given to the role of the molecular structure of the polyelectrolytes, and to the nature of the support. Also, various parameters for the preparation of multilayer films are highlighted, which are very important due to the kinetic control of the build-up process. The structure of the resulting coatings, their quality and stability, chemical reactions in the films, and potential applications are discussed.Macromol. Rapid Commun. 21, No. 7

show abstract

Section: Chemical Reactions In Films Made By Esamentioning

confidence: 99%

Section: Polymers Suited For Esamentioning

confidence: 99%

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,173

1,184

View full text Add to dashboard Cite

show abstract

“…Special focus was put on the coupling of DNA with the monolayer resulting in a one-dimensional ordering of bio-macromolecules at the charged surface. Despite a large number of publications devoted to different aspects of DNA/cationic monolayer interaction, [12][13][14][15][16][17][18][19][20][21] the influence of the chemical structure of lipid molecules as well as of the monolayer phase state on the coupling behavior is not fully understood. Therefore, emphasis was placed on a detailed physical-chemical characterization of the novel amphiphilic compounds accompanied by biological testing.…”

Section: Introductionmentioning

confidence: 99%

Physical–Chemical Properties and Transfection Activity of Cationic Lipid/DNA Complexes

et al. 2009

View full text Add to dashboard Cite

Investigation of DNA interactions with cationic lipids is of particular importance for the fabrication of biosensors and nanodevices. Furthermore, lipid/DNA complexes can be applied for direct delivery of DNA-based biopharmaceuticals to damaged cells as non-viral vectors. To obtain more effective and safer DNA vectors, the new cationic lipids 2-tetradecylhexadecanoic acid-{2-[(2-aminoethyl)amino]ethyl}amide (CI) and 2-tetradecylhexadecanoic acid-2-[bis(2-aminoethyl)amino]ethylamide (CII) were synthesized and characterized. The synthesis, physical-chemical properties and first transfection and toxicity experiments are reported. Special attention was focused on the capability of CI and CII to complex DNA at low and high subphase pH values. Langmuir monolayers at the air/water interface represent a well-defined model system to study the lipid/DNA complexes. Interactions and ordering of DNA under Langmuir monolayers of the new cationic lipids were studied using film balance measurements, grazing incidence X-ray diffraction (GIXD) and X-ray reflectivity (XR). The results obtained demonstrate the ability of these cationic lipids to couple with DNA at low as well as at high pH value. Moreover, the observed DNA structuring seems not to depend on subphase pH conditions. An influence of the chemical structure of the lipid head group on the DNA binding ability was clearly observed. Both compounds show good transfection efficacy and low toxicity in the in vitro experiments indicating that lipids with such structures are promising candidates for successful gene delivery systems.

show abstract

“…This approach utilizes the electrostatic attraction of macromolecules together with charge reversal at each step of macro-ion adsorption. Since 1991, a variety of materials such as synthetic polyelectrolytes (Decher et al 1994), proteins (Lvov et al 1995;Lvov & Sukhorukov, 1997;Onda et al 1996aOnda et al , 1996b, nucleic acids (Sukhorukov et al 1996) have been employed to assemble multilayers. The assembling of multilayers was demonstrated on different surfaces.…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of proteins by layer‐by‐layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

Balabushevitch

Sukhorukov

Moroz

et al. 2001

Biotech & Bioengineering

Self Cite

139

View full text Add to dashboard Cite

A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility.

show abstract

Multilayer films containing immobilized nucleic acids. Their structure and possibilities in biosensor applications

Cited by 122 publications

References 18 publications

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Physical–Chemical Properties and Transfection Activity of Cationic Lipid/DNA Complexes

Encapsulation of proteins by layer‐by‐layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

Contact Info

Product

Resources

About