Multilocus Genetic Techniques, RAPD-PCR and ISSR-PCR Markers and Polygalacturonase Activity as Tools for Differentiation Among Alternaria solani Isolates on Tomato Fruits and Relation to their Pathogenicity in Egypt

Adss, Ibrahim A.; Abdel-Gaye, Muhammad A.; Botros, William A.; Hafez, Elsayed E.

doi:10.3923/ajppaj.2017.18.27

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Molecular tools are the most accurate for identifying plant pathogens. Their use has increased in recent years due to the availability of specific equipment and chemicals and their ability to identify the types and aggressiveness of isolates (Adss et al, 2017). This study aimed to isolate, purify and identify F. oxysporum.…”

Section: Introductionmentioning

confidence: 99%

Differentiation between Tomato Fusarium Wilt Isolates by ISSR Markers and Virulence Analysis

Adss

El-Shahmy²,

Amer³

2022

Journal of the Advances in Agricultural Researches

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Fusarium oxysporum. f. sp. lycopersici (FOT) causes tomato fusarium wilt, one of the critical fungal diseases causing severe losses in tomato production in Egypt. Eight isolates of F. oxysporum. f. sp. lycopersici were isolated from infected tomato plants collected from six Egyptian governorates. FOT4, FOT2, and FOT5 isolates were highly virulent, while FOT6 and FOT8 were moderately virulent, and FOT7, FOT3, and FOT1 were weakly virulent. All tested ISSRs primers were polymorphic and produced 81 DNA loci of various molecular sizes. DNA fragment patterns analysis showed 37 monomorphic and 44 polymorphic bands, with 54.32% polymorphism between the tested isolates. Among the tested isolates, primer UMC-840 had the highest level of polymorphism (85.71%), while primer HB-13 had the lowest level (33.33%). Dendrogram and ISSR band patterns were analyzed using the UPGMA method to detect the relationship between the eight isolates. The highest genetic homology (82.9%) was observed between FOT4 and FOT3 isolates, while the lowest genetic homology (63.5%) was observed between FOT1 and FOT2 isolates. The eight isolates were classified into three main clusters (A, B, and C). Cluster A includes two sub-clusters; sub-cluster A1 is divided into two groups, group1 consists of two subgroups, subgroup1 include FOT 6 isolate, and subgroup 2 includes FOT4, FOT3, and FOT5 isolates (FOT4 and FOT5 were high virulent isolates), group2 has FOT8 isolate. Sub-cluster A2 includes FOT6 isolate. However, cluster B includes the FOT1 isolate, and cluster C includes the FOT2 isolate.

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Section: Introductionmentioning

confidence: 99%

Differentiation between Tomato Fusarium Wilt Isolates by ISSR Markers and Virulence Analysis

Adss

El-Shahmy²,

Amer³

2022

Journal of the Advances in Agricultural Researches

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“…Similarity matrix of isolated fungi based on ISSR analysisDendrogame was constructed using UPGMA cluster analyses to reveal the genetic relationships among five fungal isolates. Fig(6) showed four major clusters, the first group included A. flavus isolate; the second group contained A. terreus isolate; the third group included A. parasiticus and A. niger isolates; the fourth group contained A. ochraceus isolate. Similar observations were reported by Abdulateef et al (2014) who used ISSR-PCR based technology to document genetic diversity among some local Iraqi isolates through the determination of the ability A. flavus isolates to produce aflatoxin B 1 .…”

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confidence: 99%

Application of Biotechnology for the Production of New Food Preservations

Aly

Emam

2020

Int.J.Halal.Res

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The presence of pathogenic fungi has been a global concern has economical affects. Recently prevent the fungal growth using biological control using halal source become an important strategy for limiting mycotoxigenic fungi. Therefore the aim of this study that the use of some LAB bacteria and screen their antimicrobial activity against pathogenic fungi. Halal bacteria extracts were studied for the antimicrobial activity. The results indicated that two isolates were identified. Meanwhile a total of 90 fungal isolates were isolated from halal food samples. Aspergillus was the dominant fungi (65%) in five species namely, parasiticus, flavus ochraceus, terreus, and niger. Fusarium (clomorum and graminerum) and Penicillium were in lower frequency. Halal bacterial extracts were reduced the mycelium dry weight of all tested fungi and showed clear zone inhibition. Molecular identification of mycotoxin producing fungi was estimated. In conclusion, the study revealed the probability of using halal Enterococcus isolates in food preservation to ensure halal food safety. The partial or total sequencing of the 16s ribosomal DNA (rRNA) gene showed a fast technique used fungi classification

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Morphological Characterization of Colletotrichum gloeosporioiedes Identified from Anthracnose of Mangifera indica L.

Alam¹,

Adhikary²,

.³

2017

Asian J. of Plant Pathology

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Multilocus Genetic Techniques, RAPD-PCR and ISSR-PCR Markers and Polygalacturonase Activity as Tools for Differentiation Among Alternaria solani Isolates on Tomato Fruits and Relation to their Pathogenicity in Egypt

Cited by 4 publications

References 13 publications

Differentiation between Tomato Fusarium Wilt Isolates by ISSR Markers and Virulence Analysis

Differentiation between Tomato Fusarium Wilt Isolates by ISSR Markers and Virulence Analysis

Application of Biotechnology for the Production of New Food Preservations

Morphological Characterization of Colletotrichum gloeosporioiedes Identified from Anthracnose of Mangifera indica L.

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