Multilocus sequence analysis of Borrelia burgdorferi sensu lato isolates from Western Siberia, Russia and Northern Mongolia

Sabitova, Yuliya; Фоменко, Н. В.; Tikunov, Artem; Стронин, О. В.; Khasnatinov, M. A.; Davaajav, Abmed; Данчинова, Г. А.; Golovljova, Irina; Tikunova, Nina

doi:10.1016/j.meegid.2018.04.015

Cited by 17 publications

(9 citation statements)

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“…However, we propose that our results in this study case are valid from epidemiological perspective because each of these borrelias is a well-recognized agent of Lyme disease, and no other species from the B. burgdorferi s.l. complex has ever been detected in Mongolia or neighboring territories [ 18 , 33 , 34 , 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

“…and B. miyamotoi can infect the same range of tick and vertebrate hosts, co-infections with these pathogens are usual in ticks [ 30 – 32 ]. In Mongolia, the LB group is represented by the genetically diverse B. afzelii , B. garinii and B. bavariensis isolates [ 33 , 34 ], whereas all known isolates of B. miyamotoi are very similar and belong to the Siberian lineage [ 18 ]. However, due to high genetic similarity of B. garinii , B. afzelii , B. bavariensis and B. miyamotoi , their molecular identification and analysis in co-infected samples is complicated by mixed DNA/RNA templates, especially when the conserved genome regions are investigated [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Co-infections with multiple pathogens in natural populations of Ixodes persulcatus ticks in Mongolia

et al. 2022

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Background In Mongolia, the taiga tick Ixodes persulcatus is the major vector of tick-borne pathogens. Knowledge about co-infections of these pathogens in ticks is necessary both for understanding their persistence in nature and for diagnosing and treating tick-borne diseases. Methods The prevalence of seven tick-borne infections in 346 I. persulcatus collected from the Selenge and Bulgan provinces of Mongolia was evaluated using real-time PCR. Quantification of Borrelia spp. was performed using multiplex quantitative PCR targeting the 16S rRNA gene. Genetic analysis of Borrelia spp. in 11 ticks infected with Borrelia miyamotoi, including six ticks co-infected with Borrelia burgdorferi sensu lato (s.l.), was performed by high-throughput sequencing of the flaB gene fragment. Results Six ticks (1.7%) were infected with tick-borne encephalitis virus (TBEV); 171 (49.4%), with B. burgdorferi sensu lato; 17 (4.9%), with B. miyamotoi; 47 (13.6%), with Anaplasma phagocytophilum; and 56 (16.2%), with Ehrlichia sp. Neither Rickettsia sibirica nor R. heilongjiangensis were detected. Borrelia burgdorferi s.l. occurred as co-infection in 55 (32.2%) of all infected ticks. The other pathogens co-infected ticks in 58.8–70.2% of cases. No pairwise associations between co-infecting pathogens were observed, with the exception of a positive association between A. phagocytophilum and Ehrlichia sp. infections. The spirochete loads of B. miyamotoi were significantly higher than those of B. burgdorferi s.l. (mean: 5.2 vs 4.0 log10 genome copies/tick, respectively). Ten isolates of B. miyamotoi belonged to the Siberian lineage. Borrelia burgdorferi s.l was represented by nine isolates of B. afzelii, B. bavariensis and B. garinii. Conclusions In populations of I. persulcatus inhabiting the Selenge and Bulgan provinces of Mongolia, five vector-borne pathogens, i.e. TBEV, B. burgdorferi s.l., B. miyamotoi, A. phagocytophilum and Ehrlichia sp., persist independently from each other, with the exception of A. phagocytophilum and Ehrlichia sp. which seem to share the circulation mode. The discrepancies in B. burgdorferi s.l. and B. miyamotoi prevalence and spirochete load per tick suggest that different ecological niches are occupied by Lyme disease and relapsing fever agents. High-throughput sequencing allows genetic identification of borreliae species in co-infected ticks. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Co-infections with multiple pathogens in natural populations of Ixodes persulcatus ticks in Mongolia

et al. 2022

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“…Research conducted in Szczecin, in which fragments of flaB gene were amplified out of the I. ricinus isolate have demonstrated the occurrence of the following species in their tissue: B. burgdorferi, B. garinii, B. afzelii and B. miyamotoi [42]. Genotyping of the B. burgdorferi spirochaetes with the application of the p66 gene and genes of surface proteins from ticks of the Ixodes persulcatus and Ixodes pavlovskyi species was conducted by Sabitova et al in Western Syberia and Mongolia [43].…”

Section: Molecular Methodsmentioning

confidence: 99%

Current Methods Used to Identify and Genotype Spirochaetes Borreliella Burgdorferi

Teodorowicz¹,

Weiner²

2020

hpc

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Authors' contribution Wkład autorów: A. Study design/planning zaplanowanie badań B. Data collection/entry zebranie danych C. Data analysis/statistics dane-analiza i statystyki D. Data interpretation interpretacja danych E. Preparation of manuscript przygotowanie artykułu F. Literature analysis/search wyszukiwanie i analiza literatury G. Funds collection zebranie funduszy Summary Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing this disease with conventional methods have led to growing molecular biology research aimed at developing improved diagnostic tools. Molecular biology methods include all techniques that allow the detection and analysis of nucleic acids. Among them there are methods based on PCR reaction, molecular typing methods (MLST), new generation sequencing (NGS). The current development of this field gives great research opportunities. With regard to B. burgdorferi spirochaete, molecular biology is used to: confirm Lyme borreliosis, identify and type Borreliella spirochaetes, detect them in tick vectors or intermediate hosts, as well as to identify co-infections between different Borreliella species and other tick-borne pathogens. They are meant to significantly improve diagnostic analyzes. This paper reviews the current methods used for the detection and identification of B. burgdorferi. Molecular mechanisms for the survival of spirochaetes in the host, infection vectors and clinical picture of Lyme disease were also discussed.

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“…Single loci provide a genealogy only for the targeted locus not for the organism, do not buffer against exchange of genetic material and PCR may show differential sensitivity (Urwin and Maiden, 2003;Lager et al, 2017). Phylogenetic analyses of IGS sequences may give deceptive results in species assignment, as exemplified by B. afzelii, B. lanei (previously named genomospecies 2), and B. garinii/ B. bavariensis (Postic et al, 2007;Margos et al, 2009;Coipan et al, 2016;Margos et al, 2017a;Sabitova et al, 2018).…”

Section: Rrfa-rrlb (5s-23s) Igsmentioning

confidence: 99%

Multipartite Genome of Lyme Disease Borrelia: Structure, Variation and Prophages

Schwartz¹,

Margos²,

Casjens³

et al. 2022

Current Issues in Molecular Biology

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All members of the Borrelia genus that have been examined harbour a linear chromosome that is about 900 kbp in length, as well as a plethora of both linear and circular plasmids in the 5-220 kbp size range. Genome sequences for 27 Lyme disease Borrelia isolates have been determined since the elucidation of the B. burgdorferi B31 genome sequence in 1997. The chromosomes, which carry the vast majority of the housekeeping genes, appear to be very constant in gene content and organization across all Lyme disease Borrelia species. The content of the plasmids, which carry most of the genes that encode the differentially expressed surface proteins that interact with the spirochete's arthropod and vertebrate hosts, is much more variable. Lyme disease Borrelia isolates carry between 7-21 different plasmids, ranging in size from 5-84 kbp. All strains analyzed to date harbor three plasmids, cp26, lp54 and lp17. The plasmids are unusual, as compared to most bacterial plasmids, in that they contain many paralogous sequences, a large number of pseudogenes, and, in some cases, essential genes. In addition, a number of the plasmids have features indicating that they are prophages. Numerous methods have been developed for Lyme disease Borrelia strain typing. These have proven valuable for clinical and epidemiological studies, as well as phylogenomic and population genetic analyses. Increasingly, these approaches have been displaced by whole genome sequencing techniques. Some correlations between genome content and pathogenicity have been deduced, and comparative whole genome analyses promise future progress in this arena. caister.com/cimb 409 Curr. Issues Mol. Biol. Vol. 42 Curr. Issues Mol. Biol. 42: 409-454. caister.com/cimb Multipartite Genome of Borrelia Schwartz et al.

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Multilocus sequence analysis of Borrelia burgdorferi sensu lato isolates from Western Siberia, Russia and Northern Mongolia

Cited by 17 publications

References 36 publications

Co-infections with multiple pathogens in natural populations of Ixodes persulcatus ticks in Mongolia

Co-infections with multiple pathogens in natural populations of Ixodes persulcatus ticks in Mongolia

Current Methods Used to Identify and Genotype Spirochaetes Borreliella Burgdorferi

Multipartite Genome of Lyme Disease Borrelia: Structure, Variation and Prophages

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