Multilocus sequence typing of Candida tropicalis shows clonal cluster enriched in isolates with resistance or trailing growth of fluconazole

Chou, Hsiao-Hui; Lo, Hsiu‐Jung; Chen, Kuo-Wei; Liao, Ming‐Feng; Li, Shuying

doi:10.1016/j.diagmicrobio.2007.03.014

Cited by 53 publications

(56 citation statements)

References 28 publications

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“…Chen et al (32) found that in C. tropicalis, DST 164 in MLST group II was associated with a high MIC of flucytosine. In other studies, Chou et al (27) and Li et al (36) found an association between DST 140 and a reduced susceptibility to fluconazole. Chou et al (27) also reported an association between the DSTs and the C. tropicalis trailing phenomenon.…”

Section: Discussionmentioning

confidence: 81%

“…In other studies, Chou et al (27) and Li et al (36) found an association between DST 140 and a reduced susceptibility to fluconazole. Chou et al (27) also reported an association between the DSTs and the C. tropicalis trailing phenomenon. Additionally, Li et al (36) described several alleles associated with low MICs of fluconazole (alleles 3 of ICL1, 9 of MDR1, 1 of SAPT2, 3, 6, and 10 of SAPT4, 48 of XYR1, and 7 of ZWF1a).…”

Section: Discussionmentioning

confidence: 81%

“…MLST has been used to establish the relationship between the DSTs with susceptibility profile to fluconazole and the trailing phenomenon (27,32,36). In Brazil, fluconazole is an effective systemic antifungal drug against most isolates of C. tropicalis (24).…”

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confidence: 99%

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Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

et al. 2013

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fThe profiles of 61 Candida tropicalis isolates from 43 patients (28 adults and 15 children) diagnosed with candidemia at two teaching hospitals in São Paulo, Brazil, were characterized by multilocus sequence typing (MLST). For the 14 patients who had bloodstream infections, 32 isolates were serially collected from their blood and/or catheters. Thirty-nine diploid sequence types (DSTs) were differentiated. According to the C. tropicalis MLST database (http://pubmlst.org/ctropicalis/), 36 DSTs and 23 genotypes identified from the 61 isolates had not previously been described. This report represents the first study to characterize sequential isolates of C. tropicalis from candidemia cases in South America. Microvariation in a single gene was found in the sequential isolates from 7 patients. The main polymorphisms occurred in the alleles of the XYR1 gene, specifically at nucleotide positions 215, 242, and 344. Macrovariation in six gene fragments was detected in the isolates from 3 patients. eBURST analysis added two new groups to this study (groups 6 and 18). Additionally, susceptibility tests indicate that 3 isolates were resistant to fluconazole. No correlation was found between the DSTs and susceptibility to fluconazole and/or selective antifungal pressure. Two patients were sequentially infected with resistant and susceptible strains. MLST is an important tool for studying the genetic diversity of multiple/sequential isolates of patients with candidemia, allowing the comparison of our data with those from other regions of the world, as well as allowing an analysis of the genetic relationship among several clones in sequential isolates from the same or different candidemia patient sites (blood or catheter).

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 81%

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Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

et al. 2013

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“…Recently, the use of multilocus sequence typing (MLST) has been proposed to meet the increasing need for global surveillance and comparison of genotypes in a central database via the Internet. MLST of pathogenic fungal species has been developed to study population structures, such as those of Candida albicans (3,4,6), Candida tropicalis (12,42), and Candida krusei (27). A set of six genes encoding housekeeping functions, comprising the fragments FKS, LEU2, NMT1, TRP1, UGP1, and URA3, is recommended for MLST with C. glabrata (http://cglabrata.mlst.net/) (16).…”

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confidence: 99%

Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing

Lin

Chen

et al. 2007

J Clin Microbiol

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In this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and mating type class determination. Among the 25 patients with multiple isolates, 19 patients (76%) contained multiple isolates exhibiting identical or highly related PFGE and MLST genotypes, which may indicate the maintenance or microvariation of one C. glabrata strain in each patient. However, isolates from six patients (24%) displayed different sequence types, PFGE genotypes, or mating type classes, which may indicate colonization with more than one clone over time or strain replacement. High correlations among PFGE genotypes, sequence types, and mating types were found (P < 0.01). MLST exhibited less discriminatory power than PFGE with BssHII. The genotypes, sequence types, and mating type classes were independent of anatomic sources, drug susceptibility, and HIV infection status.

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“…Some studies have suggested that up to one-third of cases may occur as nosocomial clusters (3,4). Furthermore, uncommon or novel Candida species are increasingly recognized to cause candidemia (5)(6)(7)(8).…”

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confidence: 99%

Three Clustered Cases of Candidemia Caused by Candida quercitrusa and Mycological Characteristics of This Novel Species

et al. 2014

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We investigated three nosocomial Candida quercitrusa candidemia cases occurring within 2 months in a Chinese hospital. Isolates were identifiable only by DNA sequencing of the rRNA genes. Genetic (via random amplified polymorphic DNA [RAPD]) and protein mass spectral (via matrix-assisted laser desorption ionization-time of flight mass spectrometry [MALDI-TOF MS]) analyses yielded identical profiles suggesting an outbreak. The fluconazole MICs of all the strains were 16 to 32 g/ml.

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Multilocus sequence typing of Candida tropicalis shows clonal cluster enriched in isolates with resistance or trailing growth of fluconazole

Cited by 53 publications

References 28 publications

Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

Multilocus Sequence Typing of Candida tropicalis Shows the Presence of Different Clonal Clusters and Fluconazole Susceptibility Profiles in Sequential Isolates from Candidemia Patients in São Paulo, Brazil

Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing

Three Clustered Cases of Candidemia Caused by Candida quercitrusa and Mycological Characteristics of This Novel Species

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