Multilocus Sequence Typing of Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            from an Area of Low Endemicity by Real-Time PCR

Berglund, Carolina; Mölling, Paula; Sjöberg, Lennart; Söderquist, Bo

doi:10.1128/jcm.43.9.4448-4454.2005

Cited by 36 publications

(36 citation statements)

References 30 publications

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“…Isolates exhibiting SCCmec IV subtypes were found to harbor either subtype IV.1 (36% of isolates) or subtype IV.3 (20% of isolates); no isolates harbored SCCmec IV.2 or IV.4. The prevalence of subtypes IV.1 and IV.3 among pvl-positive MRSA isolates in the present study was not surprising as these are the most frequently identified subtypes among pvl-positive CA-MRSA isolates (2,17,49). Other studies also report SCCmec IV elements which fail to subtype (2,23).…”

Section: Discussionmentioning

confidence: 53%

“…The prevalence of subtypes IV.1 and IV.3 among pvl-positive MRSA isolates in the present study was not surprising as these are the most frequently identified subtypes among pvl-positive CA-MRSA isolates (2,17,49). Other studies also report SCCmec IV elements which fail to subtype (2,23). It has been suggested that SCCmec IV may be highly recombinant, especially in relation to the J1 region, and that this results in the emergence of novel subtypes.…”

Section: Discussionmentioning

confidence: 60%

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The Emergence and Importation of Diverse Genotypes of Methicillin-Resistant Staphylococcus aureus (MRSA) Harboring the Panton-Valentine Leukocidin Gene ( pvl ) Reveal that pvl Is a Poor Marker for Community-Acquired MRSA Strains in Ireland

et al. 2007

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Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) carrying pvl is an emerging problem worldwide. CA-MRSA tends to harbor staphylococcal cassette chromosome mec type IV (SCC mec IV), to be non-multiantibiotic resistant, and to have different genotypes from the local hospital-acquired MRSA (HA-MRSA). However, in Ireland, 80% of HA-MRSA isolates have the non-multiantibiotic-resistant genotype ST22-MRSA-IV. This study investigated MRSA isolates from Ireland (CA-MRSA, health care-associated MRSA, and HA-MRSA) for the carriage of pvl and determined the genotypic characteristics of all pvl -positive isolates identified. All 1,389 MRSA isolates were investigated by antibiogram-resistogram typing and SmaI DNA macrorestriction analysis. pvl -positive isolates were further characterized by multilocus sequence typing and SCC mec , agr , and toxin gene typing. Twenty-five (1.8%) MRSA isolates belonging to six genotypes (ST30, ST8, ST22, ST80, ST5, and ST154) harbored pvl . Nineteen of these (76%) were CA-MRSA isolates, but a prospective study of MRSA isolates from 401 patients showed that only 6.7% (2/30) of patients with CA-MRSA yielded pvl -positive isolates. Thus, pvl cannot be used as a sole marker for CA-MRSA. Fifty-two percent of pvl -positive MRSA isolates were recovered from patients with skin and soft tissue infections; thirty-six percent were from patients of non-Irish ethnic origin, reflecting the increasing heterogeneity of the Irish population due to immigration. All 25 pvl -positive isolates carried SCC mec IV; 14 (56%) harbored SCC mec IV.1 or IV.3, and the remaining 11 isolates could not be subtyped. This study demonstrates that pvl is not a reliable marker for CA-MRSA in Ireland and reveals the emergence and importation of diverse genotypes of pvl -positive MRSA in Ireland.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 60%

The Emergence and Importation of Diverse Genotypes of Methicillin-Resistant Staphylococcus aureus (MRSA) Harboring the Panton-Valentine Leukocidin Gene ( pvl ) Reveal that pvl Is a Poor Marker for Community-Acquired MRSA Strains in Ireland

et al. 2007

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“…The earlier researchers need to design some alternative pairs of primers, due to the possible alterations in the binding sites of reporter primers. These studies found inconsistent PCR conditions along with intra-assay variations for all seven housekeeping genes (Crisóstomo et al, 2005;Berglund et al, 2005;Huletsky et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

A new PCR strategy for multilocus sequence typing of methicillin-resistant Staphylococcus aureus

Khatoon¹,

Hussain²,

Shahid³

et al. 2013

Afr. J. Microbiol. Res.

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A single PCR protocol for methicillin-resistant S. aureus (MRSA) housekeeping genes, carbamate kinase, shikimate dehydrogenase, glycerol kinase, guanylate kinase, phosphate acetyltransferase, triosephosphate isomerases, and acetyl coenzyme-A acetyltransferase for multilocus sequence typing was developed. This PCR strategy provides a simple, rapid and cost effective amplification of the multiple loci of MRSA, with better quality data.

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“…Resistance to fusidic acid, which is characteristic of CC80 was discovered in 92% of the investigated isolates. In a Swedish study from Örebro County, CC80 and CC45 represented the predominant clonal complexes [15].…”

Section: Discussionmentioning

confidence: 99%

“…The increase of CA MRSA isolates (DK E97-1) observed recently in Denmark belonged to this clone [14]. In Sweden, CC80 is one of the most common clones [15].…”

Section: Introductionmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus Infections in Uppsala County, Sweden

Christenson¹,

Ardung²,

Sylvan³

2011

TOIDJ

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Abstract:The epidemiology of community-acquired (CA) and health care-associated (HCA) Methicillin-resistant Staphylococcus aureus (MRSA) infections were investigated in a prospective 3-year (2004-2006) surveillance study in Uppsala County. MRSA was isolated from all patients with soft tissue lesions attending hospitals and primary care clinics, as well as by systematic screening, regardless of symptoms, from all patients seeking medical care who had been treated abroad. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and spa typing were performed. The Panton-Valentine leukocidin (PVL) gene and resistance profiles were recognised. The cost of bacterial analysis was calculated. During the surveillance period, isolates were collected from 7 967 individuals of whom 82 were colonised or infected with MRSA.(24 HCA and 58 CA). A majority (65%) of the MRSA isolates originating outside Sweden. The isolates could be assigned into nine well-known international clones. The most frequent clone was CC8 (32%) within four clusters were identified and equally distributed between HCA and CA-isolates. The next most frequent clone was CC80 (DK E97-1) (23%) only discovered in CA isolates. Resistance to antibiotics other than -lactams was found in 25% of the domestic isolates and in 60 % of isolates originating abroad. None of the HCA isolates carried the virulence determinant PVL gene. There was no spread of MRSA in the community or in hospitals during the surveillance period. Most domestic cases had certain risk factors. Travellers from or family relatives in the Middle East or Asia were highly overrepresented. To save costs selected screening is recommended.

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Multilocus Sequence Typing of Methicillin-Resistant Staphylococcus aureus from an Area of Low Endemicity by Real-Time PCR

Cited by 36 publications

References 30 publications

The Emergence and Importation of Diverse Genotypes of Methicillin-Resistant Staphylococcus aureus (MRSA) Harboring the Panton-Valentine Leukocidin Gene ( pvl ) Reveal that pvl Is a Poor Marker for Community-Acquired MRSA Strains in Ireland

The Emergence and Importation of Diverse Genotypes of Methicillin-Resistant Staphylococcus aureus (MRSA) Harboring the Panton-Valentine Leukocidin Gene ( pvl ) Reveal that pvl Is a Poor Marker for Community-Acquired MRSA Strains in Ireland

A new PCR strategy for multilocus sequence typing of methicillin-resistant Staphylococcus aureus

Methicillin-Resistant Staphylococcus aureus Infections in Uppsala County, Sweden

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