Multimodal imaging and electroretinography highlights the role of VEGF in the laser-induced subretinal fibrosis of monkey

Wang, Yujiao; Fang, Qiyao; Zhang, Chaomao; Chen, Yongjiang; Gou, Tao; Cai, Qinglin; Yin, Hang; Gao, Yunxia; Feng, Yuliang; Qiu, Shuang; Zhang, Ming; Cen, Xiaobo; Zhang, Hui; Chen, Danian

doi:10.1016/j.exer.2020.108417

Cited by 6 publications

(4 citation statements)

References 52 publications

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“…Hence, it is important to clarify the molecular mechanism of anti-fibrosis and find effective anti-fibrosis targets for the treatment of HLF. With the rapid development of high-throughput sequencing technology and bioinformatics analysis, more and more lncRNAs have been identified in a variety of tissues and cells, which play key regulatory roles in a variety of biological processes [ 24 , 26 , 30 , 35 , 36 , 38 ]. Some of these lncRNAs have been shown to play anti- or pro-fibrotic functions in multiple diseases and have the potential to serve as novel preventive and therapeutic targets for fibrosis [ 11 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, we identified for the first time that XIST was dramatically upregulated in HLF tissues and obviously correlated with LF thinness and fibrosis in patients with LSCS by integrating bioinformatics technology with RT-PCR technology. Many studies have shown that XIST displays vital functions in the regulation of fibrosis [ 35 , 36 , 38 , 39 , 49 , 50 ]. In this study, functional experiments revealed that the overexpression of XIST significantly promoted HLF cells proliferation and fibrosis as well as inhibited apoptosis in vitro, while knockdown of XIST displayed an inhibitory effect on hypertrophy and fibrosis of LF in vivo and in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a study attested that VEGFA-mediated angiogenesis following mechanical stress may be a critical implementation within HLF pathological progression [ 10 ]. VEGFA is known to play a particularly important role in the regulation of tissue fibrosis [ 25 , 35 , 36 , 38 ] and autophagy [ 19 , 21 , 29 ]. Emerging studies have uncovered that VEGFA mediated autophagy to regulate the biological functions of various cells, such as granulosa cells [ 27 ], cancer cells [ 19 , 21 , 37 ], endothelial cells [ 33 ], and so on.…”

Section: Discussionmentioning

confidence: 99%

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LncRNA XIST facilitates hypertrophy of ligamentum flavum by activating VEGFA-mediated autophagy through sponging miR-302b-3p

Cao

Qiu

et al. 2023

Biol Direct

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Background Increasing evidences have shown that long non-coding RNAs (lncRNAs) display crucial regulatory roles in the occurrence and development of numerous diseases. However, the function and underlying mechanisms of lncRNAs in hypertrophy of ligamentum flavum (HLF) have not been report. Methods The integrated analysis of lncRNAs sequencing, bioinformatics analysis and real-time quantitative PCR were used to identify the key lncRNAs involved in HLF progression. Gain- and loss-function experiments were used to explore the functions of lncRNA X inactive specific transcript (XIST) in HLF. Mechanistically, bioinformatics binding site analysis, RNA pull-down, dual-luciferase reporter assay, and rescue experiments were utilized to investigate the mechanism by which XIST acts as a molecular sponge of miR-302b-3p to regulate VEGFA-mediated autophagy. Results We identified that XIST was outstandingly upregulated in HLF tissues and cells. Moreover, the up-regulation of XIST strongly correlated with the thinness and fibrosis degree of LF in LSCS patients. Functionally, knockdown of XIST drastically inhibited proliferation, anti-apoptosis, fibrosis and autophagy of HLF cells in vitro and suppressed hypertrophy and fibrosis of LF tissues in vivo. Intestinally, we uncovered that overexpression of XIST significantly promoted proliferation, anti-apoptosis and fibrosis ability of HLF cells by activating autophagy. Mechanistic studies illustrated that XIST directly medullated the VEGFA-mediated autophagy through sponging miR-302b-3p, thereby enhancing the development and progression of HLF. Conclusion Our findings highlighted that the XIST/miR-302b-3p/VEGFA-mediated autophagy axis is involved in development and progression of HLF. At the same time, this study will complement the blank of lncRNA expression profiles in HLF, which laid the foundation for further exploration of the relationship between lncRNAs and HLF in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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LncRNA XIST facilitates hypertrophy of ligamentum flavum by activating VEGFA-mediated autophagy through sponging miR-302b-3p

Cao

Qiu

et al. 2023

Biol Direct

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“…Therefore, the clinical pharmacokinetics of ocular anti-VEGF agents should be well programmed when selecting appropriate agents for individual patients. Although anti-VEGF drugs can induce significant regression of CNV and preserve central vision, but their half-life is short and multiple intravitreal injections are required to achieve sustained therapeutic effects [ 190 ]. Repeated intravitreal injections increase the risk of multiple complications and adverse effects, such as endophthalmitis, retinal detachment, and iatrogenic traumatic cataract [ 191 ].…”

Section: Current Therapeutics Strategies Targeting the Neuroglia Inte...mentioning

confidence: 99%

Crosstalk Between Microglia and Müller Glia in the Age-Related Macular Degeneration: Role and Therapeutic Value of Neuroinflammation

2024

Aging dis

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Age-related macular degeneration (AMD) is a progressive neurodegeneration disease that causes photoreceptor demise and vision impairments. In AMD pathogenesis, the primary death of retinal neurons always leads to the activation of resident microglia. The migration of activated microglia to the ongoing retinal lesion and their morphological transformation from branching to ameboid-like are recognized as hallmarks of AMD pathogenesis. Activated microglia send signals to Müller cells and promote them to react correspondingly to damaging stimulus. Müller cells are a type of neuroglia cells that maintain the normal function of retinal neurons, modulating innate inflammatory responses, and stabilize retinal structure. Activated Müller cells can accelerate the progression of AMD by damaging neurons and blood vessels. Therefore, the crosstalk between microglia and Müller cells plays a homeostatic role in maintaining the retinal environment, and this interaction is complicatedly modulated. In particular, the mechanism of mutual regulation between the two glia populations is complex under pathological conditions. This paper reviews recent findings on the crosstalk between microglia and Müller glia during AMD pathology process, with special emphasis on its therapeutic potentials.

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YAP is critical to inflammation, endothelial-mesenchymal transition and subretinal fibrosis in experimental choroidal neovascularization

Yang

Zou

Dai

et al. 2022

Experimental Cell Research

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Multimodal imaging and electroretinography highlights the role of VEGF in the laser-induced subretinal fibrosis of monkey

Cited by 6 publications

References 52 publications

LncRNA XIST facilitates hypertrophy of ligamentum flavum by activating VEGFA-mediated autophagy through sponging miR-302b-3p

LncRNA XIST facilitates hypertrophy of ligamentum flavum by activating VEGFA-mediated autophagy through sponging miR-302b-3p

Crosstalk Between Microglia and Müller Glia in the Age-Related Macular Degeneration: Role and Therapeutic Value of Neuroinflammation

YAP is critical to inflammation, endothelial-mesenchymal transition and subretinal fibrosis in experimental choroidal neovascularization

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