Multimodal molecular 3D imaging for the tumoral volumetric distribution assessment of folate-based biosensors

Abstract

The aim of this study was to characterize the in vivo volumetric distribution of three folate-based biosensors by different imaging modalities (X-ray, fluorescence, Cerenkov luminescence, and radioisotopic imaging) through the development of a tridimensional image reconstruction algorithm. The preclinical and multimodal Xtreme imaging system, with a Multimodal Animal Rotation System (MARS), was used to acquire bidimensional images, which were processed to obtain the tridimensional reconstruction. Images of mice at different times (biosensor distribution) were simultaneously obtained from the four imaging modalities. The filtered back projection and inverse Radon transformation were used as main image-processing techniques. The algorithm developed in Matlab was able to calculate the volumetric profiles of Tc-Folate-Bombesin (radioisotopic image),Lu-Folate-Bombesin (Cerenkov image), and FolateRSense™ 680 (fluorescence image) in tumors and kidneys of mice, and no significant differences were detected in the volumetric quantifications among measurement techniques. The imaging tridimensional reconstruction algorithm can be easily extrapolated to different 2D acquisition-type images. This characteristic flexibility of the algorithm developed in this study is a remarkable advantage in comparison to similar reconstruction methods.

Chemical radioanalysis of production of positron-emitting radioisotope Gallium-68 via (p,n) and (p,2n) reactions using compact cyclotron for tomography applications

“…Since radio-immunotherapy dosimetry besides nuclear imaging is a sophisticated attempt to compare the absorbed dose in tumors and normal tissues [ 64 ], up-to-date techniques are required to improve the therapeutic or diagnostic ratio [ [65] , [66] , [67] , [68] , [69] , [70] , [71] , [72] , [73] ]. Recently, bombarding 68 Zn(NO 3 ) 2 as 1 M solution in dilute (0.2–0.3 M) HNO 3 has been executed by means of GE-PET-trace cyclotron and 68 Ga liquid targets [ 74 ].…”

Theoretical and experimental characterization of emission and transmission spectra of Cerenkov radiation generated by 177Lu in tissue

“…The 177 Lu-CR images of mice acquired using the BVX, and reconstructed as per the methodology reported by Ramírez-Nava et al 21 at 2 and 24 h, are depicted in Fig. 8; images were also acquired at 4, 48, and 120 h. The quantitative analysis of the 177 Lu-CR images shows that the mean depths of the tumor and kidneys are 0.4 and 0.9 cm, respectively.…”

“…All the images were acquired and reconstructed following the methodology reported by Ramírez-Nava. 21 Briefly, CR and x-ray images of the mice were acquired, as represented in Fig. 1, with BVX and its multimodal animal rotation system (MARS) after the administration of 177 Lu (2, 4, 24, 48, and 120 h).…”

Functional characterization of polymeric organic scintillation material for preclinical imaging applications.

“…with thin phosphor screens that act as scintillators to acquire radiographic and radioisotopic images. Furthermore, BIVX is equipped with a Multimodal Animal Rotation System (MARS) that allows for the capture of 360 • rotation images [9,16].…”

“…images were acquired, setting the parameters as follows: (a) for X-ray imaging: voltage of 45 kVp, tube current of 497 μA, aluminum filter of 8 mm, exposure time of 2 seconds, and (b) for radioisotopicluminescence imaging: 30 seconds of exposure, a FOV of 7.2 cm and a 1 × 1 binning [16,19]. Considering the sensitive area of the plastic scintillators and the sample size under analysis, the acquisition process was divided into two parts: first, the upper torso of the mouse was captured, followed by the lower section.…”