Multimodal molecular imaging system for pathway-specific reporter gene expression

Rossi, Marco; Massai, Luisa; Diamanti, Daniela; Fiengo, Pasquale; Rosa, Antonella; Magrini, Roberta; Magnoni, Letizia; Chellini, Sara; Coniglio, Silvia; Diodato, Enrica; Pilli, E.; Caradonna, Nicola P.; Sardone, Gianluca; Monti, Martina; Roggeri, Riccardo; Lionetti, Vincenzo; Recchia, Fabio A.; Tunici, Patrizia; Valensin, Silvia; Scali, Carla; Pollio, Giuseppe; Porcari, Valentina

doi:10.1016/j.ejps.2016.03.006

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…Rossi et al 151 constructed a lentiviral vector combining OI and MRI together, then successfully monitored the specific biological pathway activity (Wnt pathway) in glioblastoma cells responding to LiCl treatment. After LiCl administration, the luminescence signal increased significantly and lower T2 values were observed.…”

Section: Preclinical Applications Of Multimodality Reporter Gene Imagmentioning

confidence: 99%

Multimodality reporter gene imaging: Construction strategies and application

Li¹,

Wang²,

Liu³

et al. 2018

Theranostics

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Molecular imaging has played an important role in the noninvasive exploration of multiple biological processes. Reporter gene imaging is a key part of molecular imaging. By combining with a reporter probe, a reporter protein can induce the accumulation of specific signals that are detectable by an imaging device to provide indirect information of reporter gene expression in living subjects. There are many types of reporter genes and each corresponding imaging technique has its own advantages and drawbacks. Fused reporter genes or single reporter genes with products detectable by multiple imaging modalities can compensate for the disadvantages and potentiate the advantages of each modality. Reporter gene multimodality imaging could be applied to trace implanted cells, monitor gene therapy, assess endogenous molecular events, screen drugs, etc. Although several types of multimodality imaging apparatus and multimodality reporter genes are available, more sophisticated detectors and multimodality reporter gene systems are needed.

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Section: Preclinical Applications Of Multimodality Reporter Gene Imagmentioning

confidence: 99%

Multimodality reporter gene imaging: Construction strategies and application

Li¹,

Wang²,

Liu³

et al. 2018

Theranostics

View full text Add to dashboard Cite

show abstract

Beyond luciferase-luciferin system: Modification, improved imaging and biomedical application

Jiang

Shi

Tang

et al. 2023

Coordination Chemistry Reviews

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Role of Brain Imaging in Drug Development for Psychiatry

Boer

Vries

Borra

et al. 2022

CRCEP

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Background: Over the last decades many brain imaging studies have contributed to new insights in the pathogenesis of psychiatric disease. However, in spite of these developments, progress in the development of novel therapeutic drugs for prevalent psychiatric health conditions has been limited. Objective: In this review we discuss translational, diagnostic and methodological issues that have hampered drug development in CNS disorders with a particular focus on psychiatry. The role of preclinical models is critically reviewed and opportunities for brain imaging in early stages of drug development using PET and fMRI are discussed. The role of PET and fMRI in drug development is reviewed emphasizing the need to engage in collaborations between industry, academia and phase I unitsIn this review we discuss translational, diagnostic and methodological issues that have hampered drug development in CNS disorders with a particular focus on psychiatry. The role of preclinical models is critically reviewed and opportunities for brain imaging in early stages of drug development using PET and fMRI are discussed. The role of PET and fMRI in drug development is reviewed emphasizing the need to engage in collaborations between industry, academia and phase I units. Conclusion: Brain imaging technology has revolutionized the study of psychiatric illnesses and during the last decade neuroimaging has provided valuable insights at different levels of analysis and brain organization, such as effective connectivity (anatomical), functional connectivity patterns and neurochemical information that may support both preclinical and clinical drug development. Since there is no unifying pathophysiological theory of individual psychiatric syndromes and since many symptoms cut across diagnostic boundaries, a new theoretical framework has been proposed that may help in defining new targets for treatment and thus enhance drug development in CNS diseases. In addition, it is argued that new proposals for data-mining and mathematical modelling as well as freely available databanks for neural network and neurochemical models of rodents combined with revised psychiatric classification will lead to validated new targets for drug development.

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Multimodal molecular imaging system for pathway-specific reporter gene expression

Cited by 3 publications

References 27 publications

Multimodality reporter gene imaging: Construction strategies and application

Multimodality reporter gene imaging: Construction strategies and application

Beyond luciferase-luciferin system: Modification, improved imaging and biomedical application

Role of Brain Imaging in Drug Development for Psychiatry

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