Multinucleated Giant Cell Formation and Apoptosis in Infected Host Cells Is Mediated by
            <i>Burkholderia pseudomallei</i>
            Type III Secretion Protein BipB

Suparak, Supaporn; Kespichayawattana, Wannapa; Haque, Ashraful; Easton, Anna; Damnin, Suwat; Lertmemongkolchai, Ganjana; Bancroft, Gregory J.; Korbsrisate, Sunee

doi:10.1128/jb.187.18.6556-6560.2005

Cited by 88 publications

(88 citation statements)

References 25 publications

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“…The presence of MNGCs has been observed in the tissues of patients with melioidosis (Wong et al, 1995) and we have previously shown that mutation of B. pseudomallei type III translocator protein BipB can reduce MNGC formation (Suparak et al, 2005). In contrast, none of the three plc mutants were defective in their ability to induce MNGC formation (Fig.…”

Section: B Pseudomallei Requires Plc-2 For Cell Cytotoxicitymentioning

confidence: 93%

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Characterization of two distinct phospholipase C enzymes from Burkholderia pseudomallei

et al. 2007

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Section: B Pseudomallei Requires Plc-2 For Cell Cytotoxicitymentioning

confidence: 93%

“…Apoptosis detection was performed as previously described (Suparak et al, 2005). Briefly, J774A.1 cells were infected with B. pseudomallei strains at a m.o.i.…”

Section: Methodsmentioning

confidence: 99%

Characterization of two distinct phospholipase C enzymes from Burkholderia pseudomallei

et al. 2007

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“…T3SS is important for virulence in many Gram-negative bacteria (28) and T3SS effector proteins have been implicated in hijacking the eukaryotic UPS (29). To determine whether T3SS is required for the loss of ELT-2 during B. pseudomallei infection, we enumerated nuclear ELT-2::GFP in JM90 worms infected with wild-type B. pseudomallei strains KHW or K96243 and their respective isogenic T3SS mutants in a KHW (bsaM and bspR) (30) or K96243 (bipB) (31) background that completely lack the T3SS. B. pseudomallei BsaM is homologous to Salmonella PrgH, which together with InvG and PrgK/EscJ forms the multiring base of the needle complex.…”

Section: Host Ups Mediates Elt-2 Degradation During B Pseudomalleimentioning

confidence: 99%

Burkholderia pseudomallei suppresses Caenorhabditis elegans immunity by specific degradation of a GATA transcription factor

Lee

Wong

Chin

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Burkholderia pseudomallei is a Gram-negative soil bacterium that infects both humans and animals. Although cell culture studies have revealed significant insights into factors contributing to virulence and host defense, the interactions between this pathogen and its intact host remain to be elucidated. To gain insights into the host defense responses to B. pseudomallei infection within an intact host, we analyzed the genome-wide transcriptome of infected Caenorhabditis elegans and identified ∼6% of the nematode genes that were significantly altered over a 12-h course of infection. An unexpected feature of the transcriptional response to B. pseudomallei was a progressive increase in the proportion of down-regulated genes, of which ELT-2 transcriptional targets were significantly enriched. ELT-2 is an intestinal GATA transcription factor with a conserved role in immune responses. We demonstrate that B. pseudomallei down-regulation of ELT-2 targets is associated with degradation of ELT-2 protein by the host ubiquitin-proteasome system. Degradation of ELT-2 requires the B. pseudomallei type III secretion system. Together, our studies using an intact host provide evidence for pathogen-mediated host immune suppression through the destruction of a host transcription factor.innate immunity | ubiquitin-proteosomal system

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“…After internalization, the bacterium can escape from a membrane-bound phagosome to the cytoplasm and induce actin-associated membrane protrusion, which may facilitate its spreading from cell to cell (10,12,13). Recent studies demonstrated that both type III secretion systems (T3SSs) and the global regulatory factor RpoS are involved in this process (23,26).…”

mentioning

confidence: 99%

Burkholderia pseudomallei-Induced Expression of a Negative Regulator, Sterile-α and Armadillo Motif-Containing Protein, in Mouse Macrophages: a Possible Mechanism for Suppression of the MyD88-Independent Pathway

2011

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Burkholderia pseudomallei, a causative agent of melioidosis, is a Gram-negative facultative intracellular bacterium that can survive and multiply in macrophages. Previously, we demonstrated that B. pseudomallei failed to activate gene expression downstream of the MyD88-independent pathway, particularly the expression of beta interferon (IFN-␤) and inducible nitric oxide synthase (iNOS), leading to the inability of macrophages to kill this bacterium. In the present report, we extended our study to show that B. pseudomallei was able to activate sterile-␣ and Armadillo motif (SARM)-containing protein, a known negative regulator of the MyD88-independent pathway. Both live B. pseudomallei and heat-killed B. pseudomallei were able to upregulate SARM expression in a time-dependent manner in mouse macrophage cell line RAW 264.7. The expression of SARM required bacterial internalization, as it could be inhibited by cytochalasin D. In addition, the intracellular survival of B. pseudomallei was suppressed in SARM-deficient macrophages. Increased expression of IFN-␤ and iNOS and degradation of I B␣ correlated with enhanced macrophage killing capability. These results demonstrated that B. pseudomallei modulated macrophage defense mechanisms by upregulating SARM, thus leading to the suppression of IFN-␤ and iNOS needed for bacterial elimination.

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Multinucleated Giant Cell Formation and Apoptosis in Infected Host Cells Is Mediated by Burkholderia pseudomallei Type III Secretion Protein BipB

Cited by 88 publications

References 25 publications

Characterization of two distinct phospholipase C enzymes from Burkholderia pseudomallei

Characterization of two distinct phospholipase C enzymes from Burkholderia pseudomallei

Burkholderia pseudomallei suppresses Caenorhabditis elegans immunity by specific degradation of a GATA transcription factor

Burkholderia pseudomallei-Induced Expression of a Negative Regulator, Sterile-α and Armadillo Motif-Containing Protein, in Mouse Macrophages: a Possible Mechanism for Suppression of the MyD88-Independent Pathway

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