Multinucleation in cleavage stage embryos

Royen, E Van

doi:10.1093/humrep/deg201

Cited by 188 publications

(125 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to other authors, no correlation is found between multinucleation and maternal age or the procedure performed, IVF or ICSI [3,7,8,20,25]. In contrast to general assumption, we found no difference when comparing results of pregnancies where at least one MN embryo was transferred with results on pregnancies with no MN embryos transferred.…”

Section: Discussioncontrasting

confidence: 94%

“…The presence of multinucleated (MN) blastomeres in embryos arising through IVF procedures has been described to range from 14.3% to 74% [1][2][3][4][5][6] and 43% to 87% of the patients to have MN embryos [3,4,7].…”

Section: Introductionmentioning

confidence: 99%

“…Multinucleation has been associated with developmental arrest [1-4, 7, 12-15], poor embryo morphology [1,2,7], elevated fragmentation [2,3], repeated cycles [3], blastomere asymmetry [16,17] and higher multinucleation rate on 2-cells when compared with more developed embryos [1,3,5,7,13,15,18]. Multinucleation has also been associated with higher blastomere diameter [1,6], decrease in blastocyst formation rate [5,7,15,19], reduced capacity of development on siblings of MN embryos [2,5], high amount of oocytes retrieved and elevated E 2 levels [2,3,20], follicles hypoxia [21], culture media [13,22], the use of GnRH antagonist [23] and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

“…Multinucleation has also been associated with higher blastomere diameter [1,6], decrease in blastocyst formation rate [5,7,15,19], reduced capacity of development on siblings of MN embryos [2,5], high amount of oocytes retrieved and elevated E 2 levels [2,3,20], follicles hypoxia [21], culture media [13,22], the use of GnRH antagonist [23] and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

“…Reports on MN cells that evolved to mono-nucleated blastomeres [13,25,26], eventually giving rise to normal pregnancies [2,3,7,9,27], led us to investigate the outcome of our transferred MN embryos, specifically those that "changed" from MN to mono-nucleated blastomeres (those, we named "Corrected Embryos").…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Insights on blastomere nuclearity

Gil

Gustavo

Póo

et al. 2006

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose: To analyze the results of our transferred embryos, especially those that "changed" their blastomere nuclearity from Multinucleated (MN) to Mono-nucleated during development.Methods: Pregnancies where at least one MN embryo was transferred were retrospectively evaluated and categorized in order to record and follow-up on the ones that were implanted. Embryos were classified as normal (when all blastomeres were mono-nucleated on day one and two of development), corrected (multinucleated embryos on day one that became mono-nucleated on day two) and non-corrected (multinucleated either on day one, on day two or both days).Results: There were 633 transfer cycles analyzed. Thirtythree percent (206) had at least one embryo with a MN blastomere at a given stage of development. Pregnancy and implantation rates were 29.0% and 19.0% for the group of exclusively mono-nucleated embryo transfers, and 28.6% and 15.8% for the group with at least one MN embryo transferred. The pregnancy outcome for "corrected" and "noncorrected" embryos could be corroborated unequivocally in only 9 cases, with an outcome of 8 and 4 normal babies, respectively.Conclusions: Because the amount of data analyzed is not satisfactorily large, differences were not significantly different; however, a trend may exist showing that normal at term pregnancies obtained from corrected embryos are more likely to occur than those from non-corrected embryos. Nuclear observation on a daily basis should be one of the strate-Clínica de Fertilidad, Centro Médico Docente La Trinidad, Caracas, Venezuela e-mail: mgilco@cantv.net gies used to select the best embryos for transferring, to improve implantation rates and avoid multiple pregnancies.

show abstract