Multiparticulate based thermosensitive intra-pocket forming implants for better treatment of bacterial infections in periodontitis

Yadav, Sarita; Khan, Gayasuddin; Bansal, Monika; Thokala, Sathish; Bonde, Gunjan Vasant; Upadhyay, Mansi; Mishra, Brahmeshwar

doi:10.1016/j.ijbiomac.2018.04.179

Cited by 25 publications

(20 citation statements)

References 46 publications

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“…Gum infection and inflammation are two main induced factors of periodontitis 13,14 . However, studies on periodontitis pathogenesis also revealed a considerable number of genetic factors involved in the initiation and progression of this disease 15 .…”

Section: Discussionmentioning

confidence: 99%

LncRNA DCST1‐AS1 inhibits PDLCs’ proliferation in periodontitis and may bind with miR‐21 precursor to upregulate PLAP‐1

Wang

2021

J of Periodontal Research

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Objective This study aimed to investigate the potential interactions among long noncoding RNA domain containing 1‐antisense (lncRNA DCST1‐AS1), miR‐21, and periodontal ligament–associated protein‐1 (PLAP‐1) in periodontitis. Background data discussing the present state of the field It has been verified that miR‐21 can target PLAP‐1 to regulate the osteogenic differentiation of periodontal ligament cells (PDLCs). Methods Differential expression of DCST1‐AS1 and miR‐21 in PDLCs derived from periodontitis patients and healthy controls was determined by qPCR and unpaired t test. QPCR and Western blots were conducted to evaluate the effects of overexpression of DCST1‐AS1 and miR‐21 on the expression of PLAP‐1. CCK‐8 assay was applied to evaluate the effect of DCST1‐ASI, miR‐21, or PLAP‐1 on PDLCs’ proliferation. Western blotting was conducted to detect the expression levels of CKD family (CDK4, CDK6, and CCND1). Results DCST1‐AS1 was downregulated in PDLCs derived from periodontitis patients, and its expression was inversely correlated with the expression of miR‐2 but positively correlated with PLAP‐1. Bioinformatics analysis showed that DCST1‐AS1 might bind with miR‐21 precursor but not mature miR‐21. Transfection experiments showed that overexpression of DCST1‐AS1 led to decreased expression levels of miR‐21 and significantly increased the expression levels of PLAP‐1 at both mRNA and protein levels, while overexpression of miR‐21 resulted in a dramatic lower level of PLAP‐1. CCK‐8 assay indicated that overexpression of DCST1‐AS1 or PLAP‐1 prohibited PDLCs’ proliferation. However, elevation of miR‐21 had a contrary effect on the proliferation of PDLCs. And increased expression levels of DCST1‐AS1 could significantly inhibit the expression of CDK4, CDK6, and CCND‐1, while overexpression of miR‐21 inversed the effects of DCST1‐AS1. Conclusion Therefore, the expression levels of DCST1‐AS1 are much lower in periodontitis patients compared to that in healthy controls, and overexpression of DCST1‐AS1 can significantly elevate the expression of PLAP‐1 by inhibiting miR‐21 in PDLCs.

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Section: Discussionmentioning

confidence: 99%

LncRNA DCST1‐AS1 inhibits PDLCs’ proliferation in periodontitis and may bind with miR‐21 precursor to upregulate PLAP‐1

Wang

2021

J of Periodontal Research

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“…Qu-SMs optimized batch exhibited an overall desirability of 0.795. The authenticity of the BBD for statistical optimization was proved by the obtained low percentage bias values among the predicted and the experimental response datas [24,25].…”

Section: Optimization Of Qu-smsmentioning

confidence: 93%

Design, optimization, characterization and in-vivo evaluation of Quercetin enveloped Soluplus®/P407 micelles in diabetes treatment

Singh

Moteriya

Bonde

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Quercetin (Qu), is a flavonoid known to have anti-diabetic effects owing to its antioxidant property, thus promoting regeneration of the pancreatic islets, ultimately increasing insulin secretion. But the therapeutic application of Qu is hampered by its low oral bioavailability and its unfavourable physicochemical characteristics. The present work aimed at formulation of Quercetin loaded SoluplusV R micelles (SMs) so as to enhance its bioavailability and provide prolonged release for the management of diabetes. Box-Behnken response surface methodology was employed to optimize the formulation prepared using co-solvent evaporation method. Physicochemical characterization confirmed the nanospherical nature of Quercetin loaded SoluplusV R micelles (Qu-SMs) with average particle size ranging from 85-108nm, encapsulation efficiency of 63-77%. Solid state characterization confirmed the encapsulation of Qu in the micelles without any incompatibilities. Moving forward, the results of in vitro study revealed prolonged and slow release of Qu from the developed formulations. The in vivo pharmacokinetic study revealed improved bioavailability by enveloping the drug in SMs. Moreover, the study performed to evaluate the efficiency in diabetes treatment revealed an enhanced anti-diabetic effect. Thus, Qu-SMs can serve as potential carriers aimed at improving the anti-diabetic property of Qu.

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“…Moreover, a clinical evaluation of both formulations was performed on patients suffering from periodontal pockets: The MH-loaded system, which released in vitro about 85% of the loaded drug within three days, showed the best performance, reducing pocket depth and gingival inflammation and increasing alveolar bone density. Some authors have also explored the use of in situ gelling systems, based on poloxamer, for the intra-pocket co-administration of dual antimicrobials in order to extend the antibacterial activity against a broad range of pathogens [ 82 , 83 ].…”

Section: Buccal Routementioning

confidence: 99%

Recent Advances in the Development of In Situ Gelling Drug Delivery Systems for Non-Parenteral Administration Routes

et al. 2020

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In situ gelling drug delivery systems have gained enormous attention over the last decade. They are in a sol-state before administration, and they are capable of forming gels in response to different endogenous stimuli, such as temperature increase, pH change and the presence of ions. Such systems can be administered through different routes, to achieve local or systemic drug delivery and can also be successfully used as vehicles for drug-loaded nano- and microparticles. Natural, synthetic and/or semi-synthetic polymers with in situ gelling behavior can be used alone, or in combination, for the preparation of such systems; the association with mucoadhesive polymers is highly desirable in order to further prolong the residence time at the site of action/absorption. In situ gelling systems include also solid polymeric formulations, generally obtained by freeze-drying, which, after contact with biological fluids, undergo a fast hydration with the formation of a gel able to release the drug loaded in a controlled manner. This review provides an overview of the in situ gelling drug delivery systems developed in the last 10 years for non-parenteral administration routes, such as ocular, nasal, buccal, gastrointestinal, vaginal and intravesical ones, with a special focus on formulation composition, polymer gelation mechanism and in vitro release studies.

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Multiparticulate based thermosensitive intra-pocket forming implants for better treatment of bacterial infections in periodontitis

Cited by 25 publications

References 46 publications

LncRNA DCST1‐AS1 inhibits PDLCs’ proliferation in periodontitis and may bind with miR‐21 precursor to upregulate PLAP‐1

LncRNA DCST1‐AS1 inhibits PDLCs’ proliferation in periodontitis and may bind with miR‐21 precursor to upregulate PLAP‐1

Design, optimization, characterization and in-vivo evaluation of Quercetin enveloped Soluplus®/P407 micelles in diabetes treatment

Recent Advances in the Development of In Situ Gelling Drug Delivery Systems for Non-Parenteral Administration Routes

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