Multiphoton microscopic imaging of histological sections without hematoxylin and eosin staining differentiates carcinoma <i>in situ</i> lesion from normal oesophagus

Chen, Jianxin; Xu, Jian; Kang, Deyong; Xu, Meifang; Zhuo, Shuangmu; Zhu, Xiaodong; Jiang, Xingshan

doi:10.1063/1.4826322

Cited by 33 publications

(22 citation statements)

References 20 publications

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“…23 In addition, we found that the sample processing also led to the loss of NAD(P)H and FAD, which destroyed the TPEF signals that can be used as standard biomarkers of epithelium and whose fluorescence ratio (redox ratio) was indicative of the cellular metabolic state. 23 In addition, we found that the sample processing also led to the loss of NAD(P)H and FAD, which destroyed the TPEF signals that can be used as standard biomarkers of epithelium and whose fluorescence ratio (redox ratio) was indicative of the cellular metabolic state.…”

Section: Mpm Imaging Instrumentationmentioning

confidence: 94%

“…23,25 For high-resolution imaging, a Plan-Neofluar objective (40× and NA ¼ 0.75, Zeiss) was employed in MPM examination. 23,25 For high-resolution imaging, a Plan-Neofluar objective (40× and NA ¼ 0.75, Zeiss) was employed in MPM examination.…”

Section: Mpm Imaging Instrumentationmentioning

confidence: 99%

“…18,19 In addition, label-free MPM was also demonstrated to be an efficient tool for observing dynamic cellular events under physiological conditions, allowing detailed characterization of pancreatic pathologies from observed tissue and cellular morphologies. 23 The purpose of this study is to establish the optical parameters required for imaging and diagnosis of pancreatic neoplasms without H&E staining, specifically using MPM and unstained histological sections from our tissue library. 22 Moreover, it was shown to be effective in imaging unstained tissue slices for the diagnosis of oesophageal cancer.…”

Section: Introductionmentioning

confidence: 99%

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Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains

Chen

et al. 2014

J. Biomed. Opt

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Hematoxylin and eosin (H&E) staining of tissue samples is the standard approach in histopathology for imaging and diagnosing cancer. Recent reports have shown that multiphoton microscopy (MPM) provides better sample interface with single-cell resolution, which enhances traditional H&E staining and offers a powerful diagnostic tool with potential applications in oncology. The purpose of this study was to further expand the versatility of MPM by establishing the optical parameters required for imaging unstained histological sections of pancreatic neoplasms, thereby providing an efficient and environmentally sustainable alternative to H&E staining while improving the accuracy of pancreatic cancer diagnoses. We found that the high-resolution MPM images clearly distinguish between the structure of normal pancreatic tissues compared with pancreatic neoplasms in unstained histological sections, and discernable differences in tissue architecture and cell morphology between normal versus tumorigenic cells led to enhanced optical diagnosis of cancerous tissue. Moreover, quantitative assessment of the cytomorphological features visualized from MPM images showed significant differences in the nuclear–cytoplasmic ratios of pancreatic neoplasms compared with normal pancreas, as well as further distinguished pancreatic malignant tumors from benign tumors. These results indicate that the MPM could potentially serve as an optical tool for the diagnosis of pancreatic neoplasms in unstained histological sections.

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Section: Mpm Imaging Instrumentationmentioning

confidence: 94%

Section: Mpm Imaging Instrumentationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains

Chen

et al. 2014

J. Biomed. Opt

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“…The nonlinear optical imaging system has been previously described [15,16]. In general, nonlinear optical images were obtained by an inverted microscope (LSM 510 META, Zeiss, Germany) equipped with a mode-locked femtosecond Ti: sapphire laser (Mira 900-F; Coherent, Inc.) at an excitation wavelength of 810 nm.…”

Section: Nonlinear Optical Imaging Systemmentioning

confidence: 99%

Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

Jiang

Chen

et al. 2016

Lasers Med Sci

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Neuroendocrine tumors (NETs), which are rare and slow-growing neoplasms, pose a diagnostic challenge as they are clinically silent at the time of presentation. Here, gastrointestinal neuroendocrine tumors were researched by nonlinear microscopy, and results demonstrate that this technique has the capability to identify neuroendocrine tumors in the absence of labels and can, in particular, detect rare neuroendocrine tumor cells, vascular invasion, desmoplastic reaction, and fibroelastosis induced by neuroendocrine tumors. These conclusions highlight the possibility of nonlinear optical microscopy as a diagnostic tool for label-freely differentiating neuroendocrine tumors by these histopathologic features.

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“…Recently, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) have demonstrated the potential for noninvasive visualization of tissue architecture at the cellular level [17,18]. Such methods have exhibited the ability to detect biological tissues and changes in these tissues with high resolution and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Spatial and temporal identification of cerebral infarctions based on multiphoton microscopic imaging

Wang

Lin

et al. 2018

Biomed. Opt. Express

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Ischemic stroke is a leading cause of death and permanent disability worldwide. Middle cerebral artery occlusion (MCAO) of variable duration times could be anticipated to result in varying degrees of injury that evolve spatially over time. Therefore, investigations following strokes require information concerning the spatiotemporal dimensions of the ischemic core as well as of perilesional areas. In the present study, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) was applied to image such pathophysiological events. The ischemic time-points for evaluation were set at 6, 24, 48, and 72 hours after MCAO. Our results demonstrated that MPM has the ability to not only identify the normal and ischemic brain regions, but also reveal morphological changes of the cortex and striatum at various times following permanent MCAO. These findings corresponded well with the hematoxylin and eosin (H&E) stained tissue images. With the technologic progression of miniaturized imaging devices, MPM can be developed into an effective diagnostic and monitoring tool for ischemic stroke.

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Multiphoton microscopic imaging of histological sections without hematoxylin and eosin staining differentiates carcinoma in situ lesion from normal oesophagus

Cited by 33 publications

References 20 publications

Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains

Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains

Nonlinear optical microscopy for label-free detection of gastrointestinal neuroendocrine tumors

Spatial and temporal identification of cerebral infarctions based on multiphoton microscopic imaging

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