Multiphoton microscopy: A novel diagnostic method for solid tumors in a prospective pediatric oncologic cohort, an experimental study

Muensterer, Oliver J.; Waldron, Sibylle; Boo, Yoon Jung; Ries, Claudius; Sehls, Lisa; Simon, Frank A.; Seidmann, Larissa; Birkenstock, Jérôme; Gödeke, Jan

doi:10.1016/j.ijsu.2017.10.038

Cited by 9 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pediatric tumors (nephroblastomas amongst other types of tumors) have also been assessed by MPM (41). All the typical features of pediatric tumors can be identified with distinct architectural changes on MPM.…”

Section: Diagnostic Toolmentioning

confidence: 99%

Value of multiphoton microscopy in uro-oncology: a narrative review

Treacy¹,

Khosla²,

Kyprianou³

et al. 2023

Transl Androl Urol

View full text Add to dashboard Cite

Background and Objective: Multi-photon microscopy (MPM) is a 3-dimension fluorescence imaging technique that combines the excitation of two low-energy photons, enabling less photo-bleaching and deeper penetration of the imaged tissue. Two signals are detected, autofluorescence (AF), from natural intracellular fluorophores [such as nicotinamide adenine dinucleotide phosphate (NADP) and flavine adenine dinucleotide (FAD) transformation], and second harmonic generation (SHG), a physical property of the laser enhancing non-centrosymmetric structures such as collagen fibers. MPM can give both visual and quantitative information of a fresh tissue (without the need of processing, cutting or staining the tissue), aiding in the progress towards optimizing a real-time imaging device. The objective of this review is to show the value and benefits of the use of MPM in uro-oncology.Methods: A structured literature review was performed using PubMed and Web of Sciences, including all articles with the following keywords: "multiphoton microscopy", "two-photon microscopy", "non-linear microscopy", "second harmonic generation", "urology", "prostate", "bladder", "kidney", "upper tract", "oncology", "surgical margins", "frozen section". Articles were reviewed to summarize the use of this tool in performing biopsies, assessing surgical margins, staging and grading complementary tool, and real-time imaging.

show abstract

Section: Diagnostic Toolmentioning

confidence: 99%

Value of multiphoton microscopy in uro-oncology: a narrative review

Treacy¹,

Khosla²,

Kyprianou³

et al. 2023

Transl Androl Urol

View full text Add to dashboard Cite

show abstract

“…MPM has been used to study many biomedical problems, including applications in urology (Katz et al, 2014 ), tumor assessment (Muensterer et al, 2017 ), infectious diseases (Belperron et al, 2018 ) and cardiovascular research (Wu et al, 2017 ), among others. However, MPM has been most widely used in neuroscience studies for pathophysiological assessment and follow-up of the brain.…”

Section: Technical Approaches For Brain In Vivo Mumentioning

confidence: 99%

In Vivo Imaging of Microglia With Multiphoton Microscopy

Hierro-Bujalance

Bacskai

García-Alloza

2018

Front. Aging Neurosci.

View full text Add to dashboard Cite

Neuroimaging has become an unparalleled tool to understand the central nervous system (CNS) anatomy, physiology and neurological diseases. While an altered immune function and microglia hyperactivation are common neuropathological features for many CNS disorders and neurodegenerative diseases, direct assessment of the role of microglial cells remains a challenging task. Non-invasive neuroimaging techniques, including magnetic resonance imaging (MRI), positron emission tomography (PET) and single positron emission computed tomography (SPECT) are widely used for human clinical applications, and a variety of ligands are available to detect neuroinflammation. In animal models, intravital imaging has been largely used, and minimally invasive multiphoton microcopy (MPM) provides high resolution detection of single microglia cells, longitudinally, in living brain. In this study, we review in vivo real-time MPM approaches to assess microglia in preclinical studies, including individual cell responses in surveillance, support, protection and restoration of brain tissue integrity, synapse formation, homeostasis, as well as in different pathological situations. We focus on in vivo studies that assess the role of microglia in mouse models of Alzheimer’s disease (AD), analyzing microglial motility and recruitment, as well as the role of microglia in anti-amyloid-β treatment, as a key therapeutic approach to treat AD. Altogether, MPM provides a high contrast and high spatial resolution approach to follow microglia chronically in vivo in complex models, supporting MPM as a powerful tool for deep intravital tissue imaging.

show abstract

“…As a result of their inherent optical sectioning capabilities and intrinsic contrast mechanisms, Multiphoton Microscopies (MPM) have emerged over the past three decades as very powerful tools for the label-free characterization of tissue morphology, functionality and biochemical composition [1][2][3], in-vivo and ex-vivo. Among these, Two-Photon Excitation Fluorescence (TPEF) microscopy [4] and Second-harmonic Generation (SHG) microscopy [5] have demonstrated their usefulness for exploring important properties of tissues, allowing the assessment of anatomical and functional states, and the identification of valuable pathological cues [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Multiphoton microscopy of the dermoepidermal junction and automated identification of dysplastic tissues with deep learning

Huttunen

Hristu

Dumitru

et al. 2019

Biomed. Opt. Express

View full text Add to dashboard Cite

Histopathological image analysis performed by a trained expert is currently regarded as the gold-standard for the diagnostics of many pathologies, including cancers. However, such approaches are laborious, time consuming and contain a risk for bias or human error. There is thus a clear need for faster, less intrusive and more accurate diagnostic solutions, requiring also minimal human intervention. Multiphoton microscopy (MPM) can alleviate some of the drawbacks specific to traditional histopathology by exploiting various endogenous optical signals to provide virtual biopsies that reflect the architecture and composition of tissues, both in-vivo or ex-vivo. Here we show that MPM imaging of the dermoepidermal junction (DEJ) in unstained fixed tissues provides useful cues for a histopathologist to identify the onset of non-melanoma skin cancers. Furthermore, we show that MPM images collected on the DEJ, besides being easy to interpret by a trained specialist, can be automatically classified into healthy and dysplastic classes with high precision using a Deep Learning method and existing pre-trained convolutional neural networks. Our results suggest that deep learning enhanced MPM for in-vivo skin cancer screening could facilitate timely diagnosis and intervention, enabling thus more optimal therapeutic approaches.

show abstract

Multiphoton microscopy: A novel diagnostic method for solid tumors in a prospective pediatric oncologic cohort, an experimental study

Abstract: III.

Cited by 9 publications

References 21 publications

Value of multiphoton microscopy in uro-oncology: a narrative review

Value of multiphoton microscopy in uro-oncology: a narrative review

In Vivo Imaging of Microglia With Multiphoton Microscopy

Multiphoton microscopy of the dermoepidermal junction and automated identification of dysplastic tissues with deep learning

Contact Info

Product

Resources

About