Abstract:High-resolution optical in vivo and ex vivo skin biopsies with subcellular resolution and metabolic information can be provided by multimodal multiphoton tomography. We report on the use of an ultracompact air-chilled 50/80 MHz femtosecond fiber laser for two-photon autofluorescence excitation, generation of higher harmonics, fluorescence lifetime imaging, and confocal reflectance imaging. The 18cm long laser head is placed into a 360° imaging head without requiring an optical arm. The tomograph operates effic… Show more
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