Multiple authentications of high-value milk by centrifugal microfluidic chip-based real-time fluorescent LAMP

Yu, Wenhua; Chen, Yanjing; Wang, Zhiying; Qiao, Liang; Xie, Ruibin; Zhang, Juan; Bian, Suying; Li, Hui; Zhang, Yan; Chen, Ailiang

doi:10.1016/j.foodchem.2021.129348

Cited by 43 publications

(18 citation statements)

References 29 publications

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“…The authenticity assessment of dairy products via CZE of milk proteins can be considered as one of the few applications for which the sensitivity of CE "as is" may be adequate. In fact, frauds on dairy products by substitution of the kind of milk with respect to the declared one makes economic sense at least above 10% added cow's milk [143], if not more.…”

Section: The Issue Of Sensitivitymentioning

confidence: 99%

Authenticity assessment of dairy products by capillary electrophoresis

et al. 2021

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Authenticity assessment of dairy products by capillary electrophoresisMilk and derivatives are a very important part in the diet of the world population. Products from goat, buffalo, and sheep species have a greater economic value than the cow ones, therefore, authenticity frauds by improperly adding cow's milk occur frequently: dairy products are among the seven more attractive foods for adulteration. Milk from each of the above-cited animal species has its own definite profile of whey proteins (variants of α-lactalbumin and β-lactoglobulin) and its definite profile of caseins (variants of α S1 -, α S2 -, β-, and κ-casein). Such proteins can be usefully exploited as markers of authenticity by using capillary electrophoresis which is the technique of choice for the analysis of proteins. Due to the multiple adjustable parameters that are unknown to other analytical techniques, capillary electrophoresis is able to detect frauds in milk mixtures and cheese with little use of solvents, fast analysis time, and ease of operation. This makes it attractive and competitive for routine checks that are very important to fight the adulteration market. Advantages and limitations are discussed.

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Section: The Issue Of Sensitivitymentioning

confidence: 99%

Authenticity assessment of dairy products by capillary electrophoresis

et al. 2021

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“…The isothermal amplification technologies provided an easier way for on-site detection. LAMP (Loop-mediated isothermal amplification) has been used to detect adulteration of goat milk [ 17 , 18 ], but it is easy to produce false positive amplification for the complicated design of four to six primers. Moreover, after the process of drying and dehydration, the milk powder will further lose the nucleic acid, which hinders the detection methods development for milk powder, in spite of large-market occupancy of milk powder products.…”

Section: Introductionmentioning

confidence: 99%

A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder

Huang

Liu

Zhang

et al. 2023

Foods

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Because of the serious adulteration of goat milk, the rapid on-site detection of goat milk powder adulteration is needed. In this study, the CRISPR/Cas12a detection system combined with recombinase polymerase amplification (RPA) was employed to qualitatively detect the adulteration of goat milk powder with cattle-derived components. Specific primers and crRNA were designed and screened. After the optimization of RPA and the Cas system, the RPA-CRISPR/Cas12a detection method was established. The detection can complete the rapid identification of cattle-derived components in 45 min, without the assistant of large equipment. The absolute detectability of the RPA-CRISPR/Cas12a assay could reach 10−2 ng/μL for cattle genomic DNA, and 1% (w/w) for cattle milk powder, which is suitable to meet the testing requirements for on-site detection. In total, 55 commercial goat milk powder products were collected for blind testing. The results showed that 27.3% of the samples were adulterated with cattle ingredients, revealing a serious adulteration situation in goat milk powder market. The RPA-CRISPR/Cas12a assay established in this research exhibited its potential for practical use of on-site detection to detect cow milk powder in goat milk powder and can provide reliable technical reference for combating food fraud of adulteration of goat milk products.

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“…[3] The emerging isothermal amplification techniques, especially loop-mediated isothermal amplification (LAMP), overcome the limitations of conventional PCR. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] LAMP is a protocol for nucleic acid amplification that was first introduced in 2000. [24] It is not affected by inhibitors and operates at the same temperature throughout the assay run, eliminating the need for thermal cycling and high-cost equipment.…”

Section: Introductionmentioning

confidence: 99%

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

et al. 2022

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The loop‐mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle. With its unique primer design, LAMP ensures the rapid replication of the targeted DNA region with high specificity and high efficiency. LAMP technology is used for diagnostic purposes in pathogen detection due to its ease of use, low cost, and simplicity without requiring complex equipment. A wide range of LAMP diagnostic platforms have been developed for applications in bacteria, virus, and parasitic pathogen detection. Herein, the methodology of LAMP technology and its applications in pathogen detection and SNP genotyping and mutation detection are discussed. Point‐of‐care (PoC) LAMP platforms designed with the principles of microfluidic chip technology, including LAMP‐on‐a‐chip, paper‐based LAMP, and smartphone‐based LAMP applications have been elaborated. LAMP technology represents a fast, robust, and reliable diagnostic platform for point‐of‐care testing.

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Multiple authentications of high-value milk by centrifugal microfluidic chip-based real-time fluorescent LAMP

Cited by 43 publications

References 29 publications

Authenticity assessment of dairy products by capillary electrophoresis

Authenticity assessment of dairy products by capillary electrophoresis

A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

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