Multiple Cytokine mRNA Expression in Human Mast Cells Stimulated via FcεRI

Abstract

Cross-linkage of FcεRI on human lung mast cells purified by affinity magnetic selection with monoclonal antibody YB5.B8 against c-kit (purity > 90%) expressed mRNA for multiple cytokines. There was no constitutive expression of interleukin (IL)-4 mRNA. Mast cell stimulation with anti-IgE induced IL-4 mRNA expression which appeared maxmal at 2 h and waned slowly over the next 24 h. IL-5, IL-6, IL-8 and tumour necrosis factor (TNF)-α mRNA were constitutively expressed. Mast cell activation with anti-IgE led to an increase of IL-5 and TNF-α m RN A signals within 2 h and which persisted for at least 24–48 h. On the other hand, IL-6 and IL-8 mRNA expression were not affected by anti-IgE challenge.

High-resolution tracking of cell division demonstrates differential effects of TH1 and TH2 cytokines on SCF-dependent human mast cell production in vitro:correlation with apoptosis and Kit expression

“…Results with IL-5 present differed only slightly from results when only SCF was present. It is tempting to correlate this lack of effect of IL-5 with the 18,19 By comparison, IFN-␥ and IL-4 had profound effects on cell division. IFN-␥ inhibited the division of early CD34 ϩ progenitors within the first week of culture ( Figure 2C) and subsequently promoted the apoptosis of older Kit ϩ cells ( Figure 3B).…”

The prognostic relevance of angiogenesis and mast cells in squamous cell carcinoma of the oesophagus

“…18 28 Basic fibroblast growth factor, interleukin 4 (IL-4), IL-8, tumour necrosis factor (TNF-), and TNF-are among the mediators present in mast cell granules, and are strong inducers of angiogenesis. [29][30][31][32] Mast cell deficient mice exhibit less tumour angiogenesis and tumour growth compared with mice with normal mast cell numbers. 33 Moreover, mast cells were shown to induce neovascularisation through the carcinogenesis of squamous cells.…”

Effects of theophylline, dexamethasone and salbutamol on cytokine gene expression in human peripheral blood CD4+ T-cells

“…However, the cellular origins of these cells are not known. Both mast cells and eosinophils are capable of synthesizing IL-5 [28,29] and theophylline may exert its cytokine inhibitory effects on them. Furthermore, some of the subjects in that study were receiving inhaled corticosteroids, making the interpretation of results difficult.…”