Multiple duplication of the bucentaur gene family, which recruits the APE-like domain of retrotransposon: Identification of a novel homolog and distinct cellular expression

Iwashita, Shintaro; Nakashima, Kentaro; Sasaki, Motoki; Osada, Naoki; Song, Si‐Young

doi:10.1016/j.gene.2009.01.012

Cited by 5 publications

(12 citation statements)

References 25 publications

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“…The situation is exactly the same for both the p97Bcnt/Cfdp2 and p97Bcnt2 proteins, which have calculated molecular masses of 66. 3 and 70.8 kDa, respectively (Iwashita et al, 2003(Iwashita et al, , 2009). This might be caused by their physical properties in that the three Bcnt-related proteins are intrinsically disordered proteins (IDPs).…”

Section: Intrinsic Disorder Of the Three Bcnt-related Proteins And Cementioning

confidence: 98%

“…Although we have not yet found evidence that p97Bcnt/Cfdp2 and p97Bcnt2 possess any of these activities, they have several characteristics common to mammalian APendonuclease with intrinsic disorder regions at both the N-and C-termini. Amino acid sequences in a part of the RTE domains are well conserved in all ruminants so far examined including Lesser Malay chevrotain (Iwashita et al, 2009). The central 239-amino acid region of the RTE domain (termed the core RTE domain) corresponds exactly to Endonuclease/Exonuclease/Phosphatase family members (http://www.ncbi.nlm.nih.gov/ cdd?term=Pfam03372).…”

Section: Rte Domains Of P97bcnt/cfdp2 and P97bcnt2mentioning

confidence: 99%

“…Although automated computational annotation predicted another homolog of p97Bcnt (LOC514131) in the bovine genome, its 5' UTR was different from the full-length cDNA that we isolated. Then we identified another paralog, termed p97Bcnt-2, in the adjacent region (Iwashita et al, 2009). The gene product, p97Bcnt2, is highly homologous to p97Bcnct/Cfdp2, comprising an acidic N-terminal region, a 324-amino acid RTE domain, and three IR units instead of the two in p97Bcnt/Cfdp2 in the C-terminal region (Fig.…”

Section: Identification Of Three Bcnt-related Proteinsmentioning

confidence: 99%

“…These data support the above scenario that resulted in the creation of the two paralogs, p97Bcnt/Cfdp2 and p97Bcnt-2. Furthermore, both the processed pseudogene of Bcnt/Cfdp1 and a 900-bp fragment encompassing the IR-II exon of p9Bcnt-2 map on bovine chromosome 26 (Iwashita et al, 2009). It is interesting to examine the relationship between the retrotransposon-mediated creation of novel genes and the occurrence of processed pseudogenes.…”

Section: Tandem Alignment Of Three Bcnt Gene Family Membersmentioning

confidence: 99%

“…It possesses multiple enzymic activities as a 3'-5' DNA exonuclease, (Iwashita et al, 2003), B: anti-p97Bcnt monoclonal antibodies (Nobukuni et al, 1997) and C: anti-p97Bcnt2 peptide antibody (Iwashita et al, 2009). The right panel shows the Coomassie Brilliant Blue staining pattern.…”

Section: Rte Domains Of P97bcnt/cfdp2 and P97bcnt2mentioning

confidence: 99%

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Bucentaur (Bcnt) Gene Family: Gene Duplication and Retrotransposon Insertion

Iwashita¹,

Osada²

2011

Gene Duplication

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Section: Intrinsic Disorder Of the Three Bcnt-related Proteins And Cementioning

confidence: 98%

Section: Rte Domains Of P97bcnt/cfdp2 and P97bcnt2mentioning

confidence: 99%

Section: Identification Of Three Bcnt-related Proteinsmentioning

confidence: 99%

Section: Tandem Alignment Of Three Bcnt Gene Family Membersmentioning

confidence: 99%

Section: Rte Domains Of P97bcnt/cfdp2 and P97bcnt2mentioning

confidence: 99%

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Bucentaur (Bcnt) Gene Family: Gene Duplication and Retrotransposon Insertion

Iwashita¹,

Osada²

2011

Gene Duplication

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The Bucentaur (BCNT) protein family: a long-neglected class of essential proteins required for chromatin/chromosome organization and function

et al. 2014

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The evolutionarily conserved Bucentaur (BCNT) protein superfamily was identified about two decades ago in bovines, but its biological role has long remained largely unknown. Sparse studies in the literature suggest that BCNT proteins perform important functions during development. Only recently, a functional analysis of the Drosophila BCNT ortholog, called YETI, has provided evidence that it is essential for proper fly development and plays roles in chromatin organization. Here, we introduce the BCNT proteins and comprehensively review data that contribute to clarify their function and mechanistic clues on how they may control development in multicellular organisms.

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A spatial similarity of stereochemical environments formed by amino acid residues defines a common epitope of two non-homologous proteins

Nakashima

Iwashita

Suzuki

et al. 2019

Sci Rep

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It is critical for development of high-quality antibodies in research and diagnostics to predict accurately their cross-reactivities with “off-target” molecules, which potentially induce false results. Herein, we report a good example of such a cross-reactivity for an off-target due to a stereochemical environment of epitopes, which does not simply depend on amino acid sequences. We found that significant subpopulation of a polyclonal peptide antibody against Bcnt (Bucentaur) (anti-BCNT-C antibody) cross-reacted with a completely different protein, glutamine synthetase (GS), and identified four amino acids, GYFE, in its C-terminal region as the core amino acids for the cross-reaction. Consistent with this finding, the anti-BCNT-C antibody strongly recognized endogenously and exogenously expressed GS in tissues and cultured cells by Western blotting and immunohistochemistry. Furthermore, we elucidated that the cross-reaction is caused by a spatial similarity between the stereochemical environments formed by amino acid residues, including the GYFE of GS and the GYIE of Bcnt, rather than by their primary sequences. These results suggest it is critical to comprehensively analyze antibody interactions with target molecules including off-targets with special attention to the physicochemical environments of epitope-paratope interfaces to decrease the risk of false interpretations of results using antibodies in science and clinical applications.

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Multiple duplication of the bucentaur gene family, which recruits the APE-like domain of retrotransposon: Identification of a novel homolog and distinct cellular expression

Cited by 5 publications

References 25 publications

Bucentaur (Bcnt) Gene Family: Gene Duplication and Retrotransposon Insertion

Bucentaur (Bcnt) Gene Family: Gene Duplication and Retrotransposon Insertion

The Bucentaur (BCNT) protein family: a long-neglected class of essential proteins required for chromatin/chromosome organization and function

A spatial similarity of stereochemical environments formed by amino acid residues defines a common epitope of two non-homologous proteins

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