Multiple Export Mechanisms for mRNAs

Delaleau, Mildred; Borden, Katherine L. B.

doi:10.3390/cells4030452

Cited by 66 publications

(67 citation statements)

References 165 publications

(261 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, a small subset of mRNA export is dependent on the karyopherin, Exportin 1 (Crm1). 57 Thus trypanosomes appear to have modified their NPC and the Ran-mediated export pathways, a system that already has enormous flexibility, expanding it to include all transport, including mRNA export. Why have they done this?…”

Section: Tms and Alps: Multiple Ways To Tether A Leviathanmentioning

confidence: 99%

Comparative interactomics provides evidence for functional specialization of the nuclear pore complex

Obado

Field

Rout

2017

Nucleus

View full text Add to dashboard Cite

The core architecture of the eukaryotic cell was established well over one billion years ago, and is largely retained in all extant lineages. However, eukaryotic cells also possess lineagespecific features, frequently keyed to specific functional requirements. One quintessential core eukaryotic structure is the nuclear pore complex (NPC), responsible for regulating exchange of macromolecules between the nucleus and cytoplasm as well as acting as a nuclear organizational hub. NPC architecture has been best documented in one eukaryotic supergroup, the Opisthokonts (e.g. Saccharomyces cerevisiae and Homo sapiens), which although compositionally similar, have significant variations in certain NPC subcomplex structures. The variation of NPC structure across other taxa in the eukaryotic kingdom however, remains poorly understood. We explored trypanosomes, highly divergent organisms, and mapped and assigned their NPC proteins to specific substructures to reveal their NPC architecture. We showed that the NPC central structural scaffold is conserved, likely across all eukaryotes, but more peripheral elements can exhibit very significant lineage-specific losses, duplications or other alterations in their components. Amazingly, trypanosomes lack the major components of the mRNA export platform that are asymmetrically localized within yeast and vertebrate NPCs. Concomitant with this, the trypanosome NPC is ALMOST completely symmetric with the nuclear basket being the only major source of asymmetry. We suggest these features point toward a stepwise evolution of the NPC in which a coating scaffold first stabilized the pore after which selective gating emerged and expanded, leading to the addition of peripheral remodeling machineries on the nucleoplasmic and cytoplasmic sides of the pore.

show abstract

Section: Tms and Alps: Multiple Ways To Tether A Leviathanmentioning

confidence: 99%

Comparative interactomics provides evidence for functional specialization of the nuclear pore complex

Obado

Field

Rout

2017

Nucleus

View full text Add to dashboard Cite

show abstract

“…Recent findings of ALS-related genes encoding RNA-binding proteins (RBPs) such as TDP-43 and FUS and of a reduction or loss of these RBPs from the nuclei of anterior horn cells (AHCs) in ALS patients suggest a role of RNA dysregulation in ALS pathogenesis234. RNAs and RBPs are transported between the nucleus and the cytoplasm, and the nuclear pore complex (NPC) functions as a gateway for nucleo-cytoplasmic transport of these molecules56. Disruption of nucleo-cytoplasmic transport or the dysfunction of the NPC is a predicted mechanism underlying cell death78910; and its potential role in ALS pathogenesis has also been suspected.…”

mentioning

confidence: 99%

Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons

Yamashita

Aizawa

Teramoto

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Nuclear dysfunction in motor neurons has been hypothesized to be a principal cause of amyotrophic lateral sclerosis (ALS) pathogenesis. Here, we investigated the mechanism by which the nuclear pore complex (NPC) is disrupted in dying motor neurons in a mechanistic ALS mouse model (adenosine deaminase acting on RNA 2 (ADAR2) conditional knockout (AR2) mice) and in ALS patients. We showed that nucleoporins (Nups) that constituted the NPC were cleaved by activated calpain via a Ca2+-permeable AMPA receptor-mediated mechanism in dying motor neurons lacking ADAR2 expression in AR2 mice. In these neurons, nucleo-cytoplasmic transport was disrupted, and the level of the transcript elongation enzyme RNA polymerase II phosphorylated at Ser2 was significantly decreased. Analogous changes were observed in motor neurons lacking ADAR2 immunoreactivity in sporadic ALS patients. Therefore, calpain-dependent NPC disruption may participate in ALS pathogenesis, and inhibiting Ca2+-mediated cell death signals may be a therapeutic strategy for ALS.

show abstract

“…Modulates the coupling of mRNA splicing with nucleocytoplasmic transport of mature mRNAs (Delaleau and Borden, 2015).…”

Section: Uap56/ddx39bmentioning

confidence: 99%

Cellular RNA Helicases Support Early and Late Events in Retroviral Replication

Singh

Heng

Boris‐Lawrie

2018

Retrovirus-Cell Interactions

View full text Add to dashboard Cite

Multiple Export Mechanisms for mRNAs

Cited by 66 publications

References 165 publications

Comparative interactomics provides evidence for functional specialization of the nuclear pore complex

Comparative interactomics provides evidence for functional specialization of the nuclear pore complex

Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons

Cellular RNA Helicases Support Early and Late Events in Retroviral Replication

Contact Info

Product

Resources

About