Multiple Foreign Non‐h‐2 Determinants on the Surface of a Chemically‐induced Murine Sarcoma*

Abstract

SUMMARY By immunizing BALB/c (H‐2d mice against normal tissues from C57BL/6J (H‐2b), C3Hf (H‐2k) and DBA/2 (H‐2d), but not from AKR (H‐2k) strains, resistance was induced to the subsequent challenge of the ‘syngeneic’ methyl‐cholanthrene‐induced BALB/c sarcoma ST5; lymph node cells from allo‐immune BALB/c mice were also able to exert a parallel cytotoxic effect against in vitro cultured ST5 cells. The involvement of foreign H‐2 specificities in the observed cross‐reactions was ruled out by absorptions of H‐2 monospecific sera and by the interallelic combinations used, thus suggesting that non‐H‐2 histocompatibility antigens were responsible for the above findings. By using the indirect isotopic antiglobulin assay, BALB/c anti‐C57BL/6J and anti‐C3Hf polyspecific sera were found to bind specifically to cultured ST5 cells. C57BL/6J and C3Hf, but not DBA/2, lymph node cells were able to absorb the anti‐ST5 activity of the anti‐C57BL/6J serum. These results indicated that ST5 cells expressed on their surface at least two different sets of foreign non‐H‐2 antigens: one shared by C57BL/6J and C3Hf tissues, and detected by both cell‐mediated and serological techniques; the other one belonging to DBA/2 tissues, and revealed mainly at the cell‐mediated level.

Production of a tumor-specific xenoantiserum from partially purified immunoprotective tumor antigen

“…Although not purified to homogeneity, Fr 15 displayed a 50-fold increase in specific immunoprotective activity over the crude extract [23], evoked delayed-type hypersensitivity in syngeneic hosts immunized with irradiated cells or with purified plasma membranes, and did not contain H-2 alloantigenic activity [9]. Thus, contrary to the suggestions of Invernizzi et al [8], Invernizzi and Parmiani [7], and Bowen et al [3], the TSTA on MCA-F are not related to major alloantigens [9]. Furthermore, the relative purity and absence of histocompatibility antigens may be responsible for the narrow spectrum of the antibody response induced in the xenogeneic host.…”

Antigens of Chemically Induced Tumors

“…The results of the first two experiments were essentially confirmed by the Winn test, with the transfer of immune lymphocytes together with the tumor cells, and by the hybrid test which considers the possibility of a decreased immunogenicity of tumor cells when injected into hybrid mice if their HA have been modified in such a way to be related to the alien parent of the hybrid combination. The results of the in vivo studies have been supported by in vitro tests [40,42] which indicate that TATA of some chemically induced fibrosarcomas can be formed by multiple foreign antigenic determinants.…”

Expression of alien minor histocompatibility antigens distinct from tumor‐specific transplantation antigen on a murine fibrosarcoma

“…Due to the lack of reliable serology of minor HA, the evidence for the presence of alien non-H-2 antigens of this tumor is still indirect, based on histogenetic and cellmediated cytotoxicity studies (Invernizzi et al, 1977a;Parmiani et af., 1980). In order to evaluate the relationship between TSTA and alien HA, therefore, we had to choose transplantation techniques which have been used to detect both TSTA and the minor HA of the tumor.…”

“…(H-2*), C3H.OH (H-2"'), C3H.SW (H-2'), BALB.K (H-It has been shown by serological, histogenetic and biochemical approaches that murine tumors of different etiology may express alien histocompatibility antigens of the H-2 type , whereas evidence for the presence of alien non-H-2 determinants was provided by indirect findings Invernizzi et al, 1977a;Fujiwara et at., 1978;Russell et at., 1979;Law, 1980).…”