Multiple functions of CREB-binding protein during postembryonic development: identification of target genes

Abstract

BackgroundJuvenile hormones (JH) and ecdysteroids control postembryonic development in insects. They serve as valuable targets for pest management. Hence, understanding the molecular mechanisms of their action is of crucial importance. CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including those from hormone signaling pathways through co-activation of many transcription factors. However, the role of CBP during postembryonic development in insects is not well understood. Therefore, we have studied the role of CBP in postembryonic development in Tribolium, a model coleopteran insect.ResultsCBP is ubiquitously expressed in the red flour beetle, Tribolium castaneum. RNA interference (RNAi) mediated knockdown of CBP resulted in a decrease in JH induction of Kr-h1 gene expression in Tribolium larvae and led to a block in their development. Moreover, the injection of CBP double-stranded RNA (dsRNA) showed lethal phenotypes within 8 days of injection. RNA-seq and subsequent differential gene expression analysis identified CBP target genes in Tribolium. Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins associated with growth and development. Depletion of CBP impaired the expression of several JH response genes (e.g., Kr-h1, Hairy, early trypsin) and ecdysone response genes (EcR, E74, E75, and broad complex). Further, GO enrichment analyses of the downregulated genes showed enrichment in different functions including developmental processes, pigmentation, anatomical structure development, regulation of biological and cellular processes, etc.ConclusionThese data suggest diverse but crucial roles for CBP during postembryonic development in the coleopteran model insect, Tribolium. It can serve as a target for RNAi mediated pest management of this stored product pest.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4373-3) contains supplementary material, which is available to authorized users.

Correction to: Multiple functions of CREB-binding protein during postembryonic development: identification of target genes

“…Following the publication of this article [ 1 ], the authors found that some of the primers listed in Table 1 are not correct. This mistake occurred during assembly of the primer table and the authors apologize for this error.…”

WEGO 2.0: a web tool for analyzing and plotting GO annotations, 2018 update

“…Gene Ontology (GO) was started by the GO Consortium in 1998 to focus on studies of the genome of three model organisms: Drosophila Melanogaster (fruit fly), Mus musculus (mouse) and Saccharomyces cerevisiae (brewer’s or baker’s yeast) ( 1–9 ). As a result of its unified and well-structured vocabulary, GO was quickly adopted across an array of genome projects ( 10 ), transcriptome projects ( 11–14 ), proteome projects ( 15 ) and more. GO consists of three sub-ontologies: biological process, cellular component and molecular function.…”

“…With the rapid development of high-throughput sequencing, the use of genome ( 10 ), transcriptome ( 11–14 ) and proteome ( 15 ) big data has become a major factor in downstream annotation and data analyses. In following this trend and answering user feedback, WEGO was updated to version 2.0 in 2018.…”

20‐Hydroxyecdysone receptor‐activated Bombyx mori CCAAT/enhancer‐binding protein gamma regulates the expression of BmCBP and subsequent histone H3 lysine 27 acetylation in Bo. mori

“…In Camponotus floridanus (Florida carpenter ant), CBP regulates foraging and scouting behaviours by catalysing histone acetylation (Simola et al, 2016). In Tribolium castaneum (red flour beetle), CBP regulates the juvenile hormone pathway and affects postembryonic development (Roy et al, 2017).…”