Multiple Genome Sequences of Lactobacillus plantarum Strains

Kafka, Thomas A.; Geißler, Andreas; Vogel, Rudi F.

doi:10.1128/genomea.00654-17

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…Samples were then sent to GATC Biotech (Constance, Germany) for RNA sequencing. Sequencing data were analyzed using Rockhopper software on the basis of the genome of L. plantarum TMW 1.708 (Biosample: SAMN05805046 [ 23 ]) [ 24 , 25 ]. Genes with corrected p -values lower than 0.05 were considered to show significantly different expression levels.…”

Section: Methodsmentioning

confidence: 99%

Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

et al. 2018

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Tween® 80 is a frequently used supplement of media for the cultivation of lactic acid bacteria. We investigated its effect on the cell physiology and stress tolerance of Lactobacillus (L.) plantarum. Data on the transcriptomic response to Tween 80 supplementation and its effects on cellular fatty acid profiles and growth characteristics are compared with data characterizing the effect of Tween 80, other Tween types and free fatty acids on the high hydrostatic pressure (HHP) tolerance of L. plantarum strain TMW 1.708. These include effects on cell viability, sub-lethal injury, metabolic activity, protein release and propidium iodide uptake. Tween 80 caused the downregulation of fatty acid biosynthesis and an increase in oleic acid and cyclopropane fatty acid levels in the cell membrane. Tween 20, Tween 80 and free oleic acid, but not Tween 40, Tween 60 and other free fatty acids, conferred resistance against HHP. Tween 80 diminished pressure-induced loss of metabolic activity, protein release and uptake of propidium iodide. However, loss of cell viability exceeded by far membrane permeabilization, suggesting that membrane permeabilization, which has frequently been postulated as a major factor in HHP inactivation of microbes, is not necessarily required for HHP-induced cell death of Lactobacillus plantarum.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1203-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

et al. 2018

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“…High-molecular-weight DNA was isolated from liquid cultures (late exponential growth phase) in mMRS and purified as previously described (Kafka et al, 2017 ). To obtain the whole genome shotgun sequence (WGS), the Illumina MiSeq® sequencing technology was applied in combination with the SPAdes 3.9 assembly algorithm.…”

Section: Methodsmentioning

confidence: 99%

Sucrose-Induced Proteomic Response and Carbohydrate Utilization of Lactobacillus sakei TMW 1.411 During Dextran Formation

et al. 2018

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Lactobacillus (L.) sakei belongs to the dominating lactic acid bacteria in indigenous meat fermentations, while diverse strains of this species have also been isolated from plant fermentations. We could recently show, that L. sakei TMW 1.411 produces a high molecular weight dextran from sucrose, indicating its potential use as a dextran forming starter culture. However, the general physiological response of L. sakei to sucrose as carbohydrate source has not been investigated yet, especially upon simultaneous dextran formation. To address this lack of knowledge, we sequenced the genome of L. sakei TMW 1.411 and performed a label-free, quantitative proteomics approach to investigate the sucrose-induced changes in the proteomic profile of this strain in comparison to its proteomic response to glucose. In total, 21 proteins were found to be differentially expressed at the applied significance criteria (FDR ≤ 0.01). Among these, 14 were associated with the carbohydrate metabolism including several enzymes, which enable sucrose and fructose uptake, as well as, their subsequent intracellular metabolization, respectively. The plasmid-encoded, extracellular dextransucrase of L. sakei TMW 1.411 was expressed at high levels irrespective of the present carbohydrate and was predominantly responsible for sucrose consumption in growth experiments using sucrose as sole carbohydrate source, while the released fructose from the dextransucrase reaction was more preferably taken up and intracellularly metabolized than sucrose. Genomic comparisons revealed, that operons coding for uptake and intracellular metabolism of sucrose and fructose are chromosomally conserved among L. sakei, while plasmid-located dextransucrase genes are present only in few strains. In accordance with these findings, all 59 different L. sakei strains of our strain collection were able to grow on sucrose as sole carbohydrate source, while eight of them exhibited a mucous phenotype on agar plates indicating dextran formation from sucrose. Our study therefore highlights the intrinsic adaption of L. sakei to plant environments, where sucrose is abundant, and provides fundamental knowledge regarding the use of L. sakei as starter culture for sucrose-based food fermentation processes with in-situ dextran formation.

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Structural characterization of the surface-associated heteropolysaccharide of Lactobacillus plantarum TMW 1.1478 and genetic analysis of its putative biosynthesis cluster

Prechtl

Wefers

Jakob

et al. 2018

Carbohydrate Polymers

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Multiple Genome Sequences of Lactobacillus plantarum Strains

Cited by 5 publications

References 11 publications

Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum

Sucrose-Induced Proteomic Response and Carbohydrate Utilization of Lactobacillus sakei TMW 1.411 During Dextran Formation

Structural characterization of the surface-associated heteropolysaccharide of Lactobacillus plantarum TMW 1.1478 and genetic analysis of its putative biosynthesis cluster

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