Multiple-injection affinity capillary electrophoresis to examine binding constants between glycopeptide antibiotics and peptides

Zavaleta, Jose; Chinchilla, Dinora; Martinez, Karla; Gomez, Frank A.

doi:10.1016/j.chroma.2005.10.062

Cited by 22 publications

(26 citation statements)

References 43 publications

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“…injected as a plug and electrophoresed in a buffer contained known concentration of ligand, this step was repeated in the same run and the same capillary but with different ligand concentrations from low to high, increase in the ligand concentration until having alternative arrangements of the sample plug and the ligand plug generating a single electropherogram containing a series of individual sample plugs superimposed on environments of buffer containing increasing concentrations of ligand. The binding constant was calculated from the changes in relative migration time ratio (RMTR) as a function of ligand concentration [56,104]. MIACE has already been used to determine the binding constants between several peptides and glycopeptide antibiotics [56].…”

Section: Multiple-injection Acementioning

confidence: 99%

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Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

et al. 2014

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The present review covers recent advances and important applications of affinity capillary electrophoresis (ACE). It provides an overview about various ACE types, including ACE-MS, the multiple injection mode, the use of microchips and field-amplified sample injection-ACE. The most common scenarios of the studied affinity interactions are protein-drug, protein-metal ion, protein-protein, protein-DNA, protein-carbohydrate, carbohydrate-drug, peptide-peptide, DNA-drug and antigen-antibody. Approaches for the improvements of ACE in term of precision, rinsing protocols and sensitivity are discussed. The combined use of computer simulation programs to support data evaluation is presented. In conclusion, the performance of ACE is compared with other techniques such as equilibrium dialysis, parallel artificial membrane permeability assay, high-performance affinity chromatography as well as surface plasmon resonance, ultraviolet, circular dichroism, nuclear magnetic resonance, Fourier transform infrared, fluorescence, MS and isothermal titration calorimetry.

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Section: Multiple-injection Acementioning

confidence: 99%

“…The most tested peptides are the glycopeptide antibiotics [54,55]. Zavalenta et al used multiple injection ACE to study the affinity and measure the binding constant of vancomycin, ristocetin and teicoplanin when interacting with the bacterial cell wall precursor terminus d-Ala-d-Ala [56].…”

Section: Peptide-peptide Interactionmentioning

confidence: 99%

Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

et al. 2014

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“…In the technique of multiple-injection CAE (MICAE) [122,123], separate plugs of sample containing noninteracting standards, peptide I, buffer, and peptide II, are injected into the capillary and electrophoresed. Peptides I and II migrate through the capillary at similar electrophoretic velocities and remain as distinct zones due to the buffer plug between peptides.…”

Section: Affinity Electrophoresismentioning

confidence: 99%

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

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The article brings a comprehensive survey of recent developments and applications of high-performance capillary electromigration methods, zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. New approaches to the theoretical description and experimental verification of electromigration behavior of peptides and to methodology of their separations, such as sample preparation, adsorption suppression, and detection, are presented. Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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“…CE was used to examine binding constants between eight fluoroquinolone antibiotics and BSA [60], and between different glycopeptide antibiotics and peptides [61][62][63]. Likewise, the determination of pK a values for several sulfonamide antibiotics by CE was reported [64].…”

Section: Applicationsmentioning

confidence: 99%

Recent advances in the analysis of antibiotics by CE and CEC

2007

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This article reviews the most recent developments concerning the determination of antibiotics by CE and CEC. The most employed CE separation modes were CZE and MEKC although microemulsion electrokinetic capillary chromatoghraphy was also employed. For the first time, CE was coupled to MS that was applied as a specific and confirmatory detection technique for the analysis of antibiotics. The analytical characteristics of the developed methodologies as well as the different applications reported in the literature on this subject from June 2005 until May 2007 are included in this article. To give the most relevant information on this topic, the experimental conditions employed to achieve the analysis of antibiotics by CE and CEC are provided together with the main applications performed in the pharmaceutical, agrochemical, biological, food, and environmental fields, emphacizing sample preparation requirements needed in each case.

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Multiple-injection affinity capillary electrophoresis to examine binding constants between glycopeptide antibiotics and peptides

Cited by 22 publications

References 43 publications

Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

Recent developments in CE and CEC of peptides

Recent advances in the analysis of antibiotics by CE and CEC

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