Multiple lipid transport pathways to the plasma membrane in yeast

Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast

“…However, under the same conditions, ergosterol transport from the ER to the PM is indistinguishable from that seen in the sec18-1 isogenic wild-type strain. Our results differ from those of Schnabl et al [12] who reported that sec blocks caused only partial reduction of protein and lipid transport to the PM in yeast. This indicates that ergosterol transport between the ER and PM occurs via a non-vesicular (Sec18p-independent) transport mechanism [10,13,14]; a Sec18p-independent mechanism has also been described for the transport of exogenously supplied sterol from the PM to the ER [15].…”

A phosphatidylserine decarboxylase activity in root cells of oat (Avena sativa) is involved in altering membrane phospholipid composition during drought stress acclimation

“…Different mechanisms for transport of phospholipids to the plasma membrane have been proposed over the years. Vesicular traffic of lipids from the ER via Golgi to the plasma membrane, or lipid transport aided by transfer/ exchange proteins have been considered as important mechanisms [38], but evidence is accumulating that additional transport routes exist [26,35]. Recently, a direct transfer of lipids in regions of close contact between the ER and the plasma membrane was demonstrated in yeast [21], and transfer of lipid monomers to the plasma membrane in the form of lyso derivatives is another possibility [16].…”

A signal comprising a basic cluster and an amphipathic α-helix interacts with lipids and is required for the transport of Ist2 to the yeast cortical ER

“…One example of such direct transport is the exchange of lipids between the ER and plasma membrane. Experiments in Saccharomyces cerevisiae show that a non-vesicular exchange of lipids occurs between the ER and plasma membrane (Baumann et al, 2005;Raychaudhuri et al, 2006;Schnabl et al, 2005). The yeast cortical ER is in contact with the plasma membrane at many positions, where such a direct exchange of molecules between cortical ER and plasma membrane might occur (Perktold et al, 2007;Pichler et al, 2001).…”