Multiple Mechanisms Are Involved in Repression of Filamentous Phage SW1 Transcription by the DNA-Binding Protein FpsR

Jian, Huahua; Xu, Guanpeng; Liu, Shunzhang; Hao, Yali; Meng, Canxing; Xu, Jianrong; Zhang, Yue; Liu, Xipeng; Xiao, Xiang

doi:10.1016/j.jmb.2019.01.040

Cited by 7 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One non‐vibrio filamentous phage, SW1, was seen to chronically infect the deep‐sea bacterium Shewanella piezotolerans WP3. It regulated phage production via a phage‐encoded repressor, FpsR, with limited homology to other phage repressors (Jian et al ., 2019). Like filamentous vibriophages, SW1 infections have been proposed to influence host fitness by altering host gene expression.…”

Section: Phage–host Interactions Of Cultured Model Marine Systemsmentioning

confidence: 99%

Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence

Tuttle

Buchan

2020

Environmental Microbiology

View full text Add to dashboard Cite

In the oceans, viruses that infect bacteria (phages) influence a variety of microbially mediated processes that drive global biogeochemical cycles. The nature of their influence is dependent upon infection mode, be it lytic or lysogenic. Temperate phages are predicted to be prevalent in marine systems where they are expected to execute both types of infection modes. Understanding the range and outcomes of temperate phage-host interactions is fundamental for evaluating their ecological impact. Here, we (i) review phage-mediated rewiring of host metabolism, with a focus on marine systems, (ii) consider the range and nature of temperate phage-host interactions, and (iii) draw on studies of cultivated model systems to examine the consequences of lysogeny among several dominant marine bacterial lineages. We also readdress the prevalence of lysogeny among marine bacteria by probing a collection of 1239 publicly available bacterial genomes, representing cultured and uncultivated strains, for evidence of complete prophages. Our conservative analysis, anticipated to underestimate true prevalence, predicts 18% of the genomes examined contain at least one prophage, the majority (97%) were found within genomes of cultured isolates. These results highlight the need for cultivation of additional model systems to better capture the diversity of temperate phagehost interactions in the oceans.

show abstract

Section: Phage–host Interactions Of Cultured Model Marine Systemsmentioning

confidence: 99%

Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence

Tuttle

Buchan

2020

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Correspondingly, eight putative PT modification motifs in the fpsR-fpsA intergenic region were identified according to the conserved PT modification sites (5′-G ps AAC-3′/5′-G ps TTC-3′) of S. enterica serovar Cerro 87 29 . Furthermore, two of these motifs were located within FpsR operators, which are responsible for the genetic switch of phage activation 30 . Exposure of pSW2Dnd from WP3NR to peracetic acid (PAA) and Tris-acetate EDTA (TAE) buffer caused extensive DNA cleavage (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The binding of the phage-borne repressor FpsR to its cognate operator is the key controlling factor for the SW1 genetic switch 30 . To further explore the underlying mechanism through which PT modification affects phage gene expression, 8 PT modification motifs located within the fpsR-fpsA intergenic region were point mutated in pSW2Dnd and pSW2DndΔE to generate two vectors: pSW2Dnd-IG and pSW2DndΔE-IG (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes

Jian

et al. 2021

Nat Commun

Self Cite

View full text Add to dashboard Cite

Phosphorothioate (PT) modification by the dnd gene cluster is the first identified DNA backbone modification and constitute an epigenetic system with multiple functions, including antioxidant ability, restriction modification, and virus resistance. Despite these advantages for hosting dnd systems, they are surprisingly distributed sporadically among contemporary prokaryotic genomes. To address this ecological paradox, we systematically investigate the occurrence and phylogeny of dnd systems, and they are suggested to have originated in ancient Cyanobacteria after the Great Oxygenation Event. Interestingly, the occurrence of dnd systems and prophages is significantly negatively correlated. Further, we experimentally confirm that PT modification activates the filamentous phage SW1 by altering the binding affinity of repressor and the transcription level of its encoding gene. Competition assays, concurrent epigenomic and transcriptomic sequencing subsequently show that PT modification affects the expression of a variety of metabolic genes, which reduces the competitive fitness of the marine bacterium Shewanella piezotolerans WP3. Our findings strongly suggest that a series of negative effects on microorganisms caused by dnd systems limit horizontal gene transfer, thus leading to their sporadic distribution. Overall, our study reveals putative evolutionary scenario of the dnd system and provides novel insights into the physiological and ecological influences of PT modification.

show abstract

“…有趣的是, 这两个启动子之后均包含了很长的 5' 非翻译区(5' UTR), 它们在 SW1 的低温诱导中发挥者显著的作用；此外，在 fpsR 基因的下游还存在 3' UTR, 但其功能还不清楚 [75] . SW1 自身编码的 FpsR 蛋白可与相应的操纵子结合, 通过"空间位阻"和"路障"两种机 A c c e p t e d https://engine.scichina.com/doi/10.1360/SSV-2021-0256 郝亚丽等: 丝状病毒-被忽视的原核生物病毒"潜伏者" 制调节病毒基因的转录, 从而在 SW1 基因开关的转换中发挥关键作用 [76] . 最近，我们的研究发现 SW1 基因开关的诱导依赖于一个阈值温度(4º C), 且这种依赖性并不与温度梯度成反比 [41] (图 4).…”

Section: 诱导调控机制unclassified

<italic>Inoviruses</italic>—the neglected “lurkers” in prokaryotic viruses

Hao¹,

Xu²,

Xi³

et al. 2022

Sci. Sin.-Vitae

Self Cite

View full text Add to dashboard Cite

Inoviruses are highly diverse and globally prevalent, and the prokaryotes they infect cover almost all bacteria phyla, even including some archaea. After nearly sixty years of study on the typical Inoviruses represented by M13 and CTXΦ, it has been shown to possess unique biological characteristics (e.g. chronic infection) as well as important application value. In this reviews, we firstly summarized the isolation and environmental distributions of Inoviruses, and then described their genome feature, virion structure, life cycle, induction and regulatory mechanism, the physiological influences on their hosts, and their applications in different fields. Finally, prospects and directions for future investigation are proposed based on current situation of Inovirus research. We believe that further study on Inoviruses will significantly promote the understanding of prokaryotic virus-host interactions and their potential ecological functions, Meanwhile, these efforts will undoubtedly expand the depth and range of the application of Inoviruses in the field of human health and environmental science.

show abstract

Multiple Mechanisms Are Involved in Repression of Filamentous Phage SW1 Transcription by the DNA-Binding Protein FpsR

Cited by 7 publications

References 49 publications

Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence

Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence

The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes

<italic>Inoviruses</italic>—the neglected “lurkers” in prokaryotic viruses

Contact Info

Product

Resources

About

Multiple Mechanisms Are Involved in Repression of Filamentous Phage SW1 Transcription by the DNA-Binding Protein FpsR

Cited by 7 publications

References 49 publications

Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence

Lysogeny in the oceans: Lessons from cultivated model systems and a reanalysis of its prevalence

The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes

&lt;italic&gt;Inoviruses&lt;/italic&gt;&mdash;the neglected &ldquo;lurkers&rdquo; in prokaryotic viruses

Contact Info

Product

Resources

About

<italic>Inoviruses</italic>—the neglected “lurkers” in prokaryotic viruses