Multiple resistance, detoxifying enzyme activity, and inheritance of abamectin resistance in Tetranychus urticae Koch (Acarina: Tetranychidae)

Yorulmaz, Sibel; Ay, Recep

doi:10.3906/tar-0809-15

Cited by 14 publications

(6 citation statements)

References 24 publications

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“…Here, we investigated the genetic basis of abamectin resistance in two genetically unrelated populations of the two-spotted spider Different modes of inheritance of abamectin resistance have been found across populations of T. urticae, including incompletely recessive, recessive or incompletely dominant (Dermauw et al, 2012;He et al, 2009;Kwon et al, 2010;Salman & Ay, 2009). Here, independent crosses of two resistant lines with a susceptible line each yielded low levels of resistance to abamectin in the F1 generation, one and two orders of magnitude lower than the resistant parents of gBSA and aBSA respectively (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest

Villacis‐Perez

Xue

Vandenhole

et al. 2023

Evolutionary Applications

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Pesticide resistance relies on a myriad of mechanisms, ranging from single mutations to a complex and polygenic architecture, and it involves mechanisms such as targetsite insensitivity, metabolic detoxification, or a combination of these, with either additive or synergistic effects. Several resistance mechanisms against abamectin, a macrocyclic lactone widely used in crop protection, have been reported in the cosmopolitan pest Tetranychus urticae. However, it has been shown that a single mechanism cannot account for the high levels of abamectin resistance found across different mite populations. Here, we used experimental evolution combined with bulked segregant analyses to map quantitative trait loci (QTL) associated with abamectin resistance in two genetically unrelated populations of T. urticae. In these two independent QTL mapping experiments, three and four QTLs were identified, of which three were shared between experiments. Shared QTLs contained genes encoding subunits of the glutamate-gated chloride channel (GluCl) and harboured previously reported mutations, including G314D in GluCl1 and G326E in GluCl3, but also novel resistance candidate loci, including DNA helicases and chemosensory receptors. Surprisingly, the fourth QTL, present only in only one of the experiments and thus unique for one resistant parental line, revealed a non-functional variant of GluCl2, suggesting gene knock-out as resistance mechanism. Our study uncovers the complex basis of abamectin resistance, and it highlights the intraspecific diversity of genetic mechanisms underlying resistance in a cosmopolitan pest.

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Section: Discussionmentioning

confidence: 99%

Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest

Villacis‐Perez

Xue

Vandenhole

et al. 2023

Evolutionary Applications

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“…An incompletely recessive trait was found in the abamectin‐resistant strains of T. urticae , P. xylostella and house fly Musca domestica L. (Diptera: Muscidae) 43,44 . However, incomplete dominance was reported in other studies, in the abamectin resistance of B. tabaci , the NIL‐Aba and ABA strains of T. urticae , and the TH‐Abm strain of P. xylostella 11,45–47 . The difference of dominance or recessive may be affected by different genetic background and environmental conditions 48 .…”

Section: Discussionmentioning

confidence: 89%

“…43,44 However, incomplete dominance was reported in other studies, in the abamectin resistance of B. tabaci, the NIL-Aba and ABA strains of T. urticae, and the TH-Abm strain of P. xylostella. 11,[45][46][47] The difference of dominance or recessive may be affected by different genetic background and environmental conditions. 48 In addition, the number of genes controlling the insecticide resistance determined the development of resistance.…”

Section: Discussionmentioning

confidence: 99%

Cross‐resistance, inheritance and biochemical mechanism of abamectin resistance in a field‐derived strain of the citrus red mite, Panonychus citri (Acari: Tetranychidae)

Liu,

Li,

Pan

et al. 2023

Pest Management Science

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BACKGROUNDThe citrus red mite, Panonychus citri (McGregor), a global pest of citrus, has developed different levels of resistance to various acaricides in the field. Abamectin is one of the most important insecticides/acaricides worldwide that target a wide number of insect and mite pests. The evolution of abamectin resistance in P. citri is threatening the sustainable use of abamectin for mite control.RESULTSThe abamectin resistant strain (NN‐Aba), derived from a field strain NN by consistent selection with abamectin, showed 4,279‐fold resistance to abamectin compared to a relatively susceptible strain (SS) of P. citri. Cross‐resistance of NN‐Aba was observed between abamectin and emamectin benzoate, pyridaben, fenpropathrin, as well as cyflumetofen. Inheritance analyses indicated that abamectin resistance in the NN‐Aba strain was autosomal, incompletely recessive and polygenic. The synergy experiment showed that abamectin toxicity was synergized by piperonyl butoxide (PBO), diethyl maleate (DEM), and tributyl phosphorotrithiotate (TPP) in the NN‐Aba strain, and synergy ratios were 2.72‐, 2.48‐, and 2.13‐fold, respectively. The glutathione S‐transferases activity in the NN‐Aba strain were significantly increased by 2.08‐fold compared with the NN strain.CONCLUSIONThe abamectin resistance was autosomal, incompletely recessive and polygenic in P. citri. The NN‐Aba strain showed cross‐resistance to various acaricides with different modes of action. Metabolic detoxification mechanism participated in abamectin resistance in NN‐Aba strain. These findings provide useful information for resistance management of P. citri in the field.This article is protected by copyright. All rights reserved.

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“…Yorulmaz and Ay reported that the sensitivity of susceptible and resistant strains of T. urticae to abamectin acaricide was investigated in terms of the enzyme activities of esterase, GST, and monooxygenase (P450). No substantial change in esterase activities was identified for the abamectin-resistant strain paralleled to the susceptible strain (Yorulmaz & Ay, 2009). After 12 cycles of exposure to a susceptible strain of T. urticae to chlorfenapyr, the resistance ratio was found to be 580 based on the LC 50 s (Van Leeuwen et al, 2004).…”

Section: Discussionmentioning

confidence: 90%

Toxicity, joint action effect, and enzymatic assays of abamectin, chlorfenapyr, and pyridaben against the two-spotted spider mite Tetranychus urticae

Badawy

Mahmoud

Khattab

2022

JoBAZ

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Background In the present study, the comparative toxicity of three different acaricides (abamectin, chlorfenapyr, and pyridaben) in technical and formulated forms was assessed on the eggs and adult females of a susceptible strain of Tetranychus urticae. Joint toxic effects of the tested acaricides were also performed against eggs and adults. In addition, the in vitro assay of the tested acaricides was evaluated against some target enzymes isolated from the adult females. Results The LC50 values against eggs by leaf-disk-dip technique were estimated to be 294.27, 1032.93, and 9550.54 mg/L for abamectin, chlorfenapyr, and pyridaben, respectively. However, the LC50 values were 783.06, 666.55, and 731.36 mg/L for the formulations Agromectin, Challenger, and Sanmite, respectively. Abamectin was found to be the greatest lethal to the adults (LC50 = 5.39 mg/L) followed by chlorfenapyr (LC50 = 106.51 mg/L) after 24 h of the treatment by slide-dip technique. Pyridaben was least toxic (LC50 = 690.23 mg/L). Agromectin (LC50 = 0.94 mg/L) followed by Challenger (LC50 = 73.65 mg/L) while the Sanmite was the lowest toxic one (LC50 = 1160.60 mg/L) against the adults. The results of joint toxic action proved that all combinations between the technical or formulated acaricides exhibited potentiation effect and the toxicity was increased significantly against eggs and adults of T. urticae compared to the individual pesticide. The activity of acetylcholinesterase (AChE), adenosine triphosphatase (ATPase), acid and alkaline phosphatases (ACP and ALP), carboxylesterase (CaE), gamma-aminobutyric acid transaminase (GABA-T), and glutathione-S-transferase (GST) isolated from adults treated with 0.025, 0.05, 0.1, 0.5, 1, and 5 mg/L were significantly inhibited compared to the control. Conclusion This study provides the theoretical basis for a rational application of abamectin, chlorfenapyr, and pyridaben mixtures in T. urticae control.

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Multiple resistance, detoxifying enzyme activity, and inheritance of abamectin resistance in Tetranychus urticae Koch (Acarina: Tetranychidae)

Cited by 14 publications

References 24 publications

Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest

Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest

Cross‐resistance, inheritance and biochemical mechanism of abamectin resistance in a field‐derived strain of the citrus red mite, Panonychus citri (Acari: Tetranychidae)

Toxicity, joint action effect, and enzymatic assays of abamectin, chlorfenapyr, and pyridaben against the two-spotted spider mite Tetranychus urticae

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