Multiple resonance fiber-optic sensor with time division multiplexing for multianalyte detection

Abstract

A proof-of-concept multiwindow fiber-optic sensor utilizing multiple particle plasmon resonance (PPR) of silver nanoparticles and gold nanorods separately on two unclad portions of the fiber for multianalyte detection is demonstrated. The detection is based on intensity interrogation of multiple wavelengths by a single detector. Time division multiplexing is employed to modulate the illumination of dual-wavelength LEDs to induce PPRs for simultaneous real-time and label-free monitoring of two types of biomolecular interactions. Preliminary results reveal that a refractive index resolution of 9 ×10(-6) RIU is achieved. Moreover, the measured intensities of two windows independently respond to their respective binding events. The potential of the sensor architecture with multiple sensing windows for cascaded, higher throughput, and multianalyte biochemical detection can be expected.

Dual-channel fiber surface plasmon resonance biological sensor based on a hybrid interrogation of intensity and wavelength modulation

“…A two-cascaded LSPR fiber sensor based on intensity interrogation has been demonstrated to simultaneously detect two analytes. 16 However, intensity interrogation is susceptible to stability of the light source, and its fabrication process is relatively complex. Other SPR sensors based on fiber-optic bundles 17 to realize multichannel detection limit the miniaturization of the sensing element, and the procedure is complex.…”

Multichannel Fiber Optic SPR Sensors: Realization Methods, Application Status, and Future Prospects

“…Meanwhile, the MC‐SPR sensors are also widely applied in the high‐precision detection of other biological macromolecules. [ 113,120,182–184 ] In 2012, Lin et al. cascaded two different nanometal channels to achieve resonance at two specific wavelengths, and successfully detected two proteins using TDM technology [182] .…”

“…[ 113,120,182–184 ] In 2012, Lin et al. cascaded two different nanometal channels to achieve resonance at two specific wavelengths, and successfully detected two proteins using TDM technology [182] . The AgNPs channel adopts the specific binding properties of N‐(2,4‐dinitrophenyl)‐6‐aminohexanoic acid (DNP) and the antibody to detect anti‐DNP, and the detection limit of 4.5 × 10 −10 g mL −1 is obtained.…”

Slab waveguide-based particle plasmon resonance optofluidic biosensor for rapid and label-free detection

“…Based on this phenomenon, several WG biosensors have been developed. 15,26–28 However, it is still challenging to use WG sensors for biosensing.…”

“…34 Through the marriage of WG and plasmonics, several biosensors have been developed. 26–28,35 However, the previously reported devices suffer from time consuming fabrication processes, insufficient sensitivities, high cost, and/or bulky measurement systems.…”