Multiple roles for a novel RND‐type efflux system in <i>Acinetobacter baumannii</i> AB5075

Tipton, Kyle A.; Farokhyfar, Marjan; Rather, Philip N.

doi:10.1002/mbo3.418

Cited by 32 publications

(34 citation statements)

References 54 publications

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“…In A. baumannii, some members of the RND superfamily have been implicated in the pathogenicity of the bacterium. For example, in A. baumannii strain AB5075, the attenuated virulence of two mutants (arpA and arpB) lacking components of an RND system was demonstrated in the G. mellonella model (22). Furthermore, inactivation of the gene A1S_0114, involved in the synthesis of a lipopeptide, reduces the motility and virulence of A. baumannii.…”

Section: Discussionmentioning

confidence: 99%

Roles of Efflux Pumps from Different Superfamilies in the Surface-Associated Motility and Virulence of Acinetobacter baumannii ATCC 17978

Pérez-Varela

Corral

Aranda

et al. 2019

Antimicrob Agents Chemother

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Although the relationship between Acinetobacter baumannii efflux pumps and antimicrobial resistance is well documented, less is known about the involvement of these proteins in the pathogenicity of this nosocomial pathogen. In previous work, we identified the AbaQ major facilitator superfamily (MFS) efflux pump and demonstrated its participation in the motility and virulence of A. baumannii. In the present study, we examined the role in these processes of A. baumannii transporters belonging to different superfamilies of efflux pumps. Genes encoding known or putative permeases belonging to efflux pump superfamilies other than the MFS were selected, and the corresponding knockouts were constructed. The antimicrobial susceptibilities of these mutants were consistent with previously reported data. In mutants of A. baumannii strain ATCC 17978 carrying inactivated genes encoding the efflux pumps A1S_2736 (resistance nodulation division [RND]), A1S_3371 (multidrug and toxic compound extrusion [MATE]), and A1S_0710 (small multidrug resistance [SMR]), as well as the newly described ATP-binding cassette (ABC) permeases A1S_1242 and A1S_2622, both surface-associated motility and virulence were reduced compared to the parental strain. However, inactivation of the genes encoding the known ABC permeases A1S_0536 and A1S_1535, the newly identified putative ABC permeases A1S_0027 and A1S_1057, or the proteobacterial antimicrobial compound efflux (PACE) transporters A1S_1503 and A1S_2063 had no effects on bacterial motility or virulence. Our results demonstrate the involvement of antimicrobial transporters belonging at least to five of the six known efflux pump superfamilies in both surface-associated motility and virulence in A. baumannii ATCC 17978.

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Section: Discussionmentioning

confidence: 99%

Roles of Efflux Pumps from Different Superfamilies in the Surface-Associated Motility and Virulence of Acinetobacter baumannii ATCC 17978

Pérez-Varela

Corral

Aranda

et al. 2019

Antimicrob Agents Chemother

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“…RND efflux systems, a category of bacterial efflux pumps, play a prominent role in both intrinsic and acquired multidrug resistance identified in Gram-negative bacteria and catalyze the active efflux of a wide variety of antibacterial substrates including antibiotics and chemotherapeutic agents [65,66]. RND efflux systems are normally composed of three proteins: an inner membrane transporter, an outer membrane protein that functions as a pore, and a periplasmic protein that interacts with both the inner and outer membrane proteins to provide a conduit for the extrusion of small molecules [67].…”

Section: Rnd and Abc Efflux Systemsmentioning

confidence: 99%

Genomic and Metabolic Insights into Denitrification, Sulfur Oxidation, and Multidrug Efflux Pump Mechanisms in the Bacterium Rhodoferax sediminis sp. nov.

Jin

Zhuo

et al. 2020

Microorganisms

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This genus contains both phototrophs and nonphototrophic members. Here, we present a high-quality complete genome of the strain CHu59-6-5 T , isolated from a freshwater sediment. The circular chromosome (4.39 Mbp) of the strain CHu59-6-5 T has 64.4% G+C content and contains 4240 genes, of which a total of 3918 genes (92.4%) were functionally assigned to the COG (clusters of orthologous groups) database. Functional genes for denitrification (narGHJI, nirK and qnor) were identified on the genomes of the strain CHu59-6-5 T , except for N 2 O reductase (nos) genes for the final step of denitrification. Genes (soxBXAZY) for encoding sulfur oxidation proteins were identified, and the FSD and soxF genes encoding the monomeric flavoproteins which have sulfide dehydrogenase activities were also detected. Lastly, genes for the assembly of two different RND (resistance-nodulation division) type efflux systems and one ABC (ATP-binding cassette) type efflux system were identified in the Rhodoferax sediminis CHu59-6-5 T . Phylogenetic analysis based on 16S rRNA sequences and Average Nucleotide Identities (ANI) support the idea that the strain CHu59-6-5 T has a close relationship to the genus Rhodoferax. A polyphasic study was done to establish the taxonomic status of the strain CHu59-6-5 T . Based on these data, we proposed that the isolate be classified to the genus Rhodoferax as Rhodoferax sediminis sp. nov. with isolate CHu59-6-5 T .

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“…In particular, several regulatory genes, including RND-type efflux system, have been reported to be involved in the resistance and virulence of A . baumannii [ 13 ]. For example, the OmpA protein is involved in biofilm formation and virulence [ 14 ], it also can increase resistance to chloramphenicol, aztreonam, and nalidixic acid [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The role of the type VI secretion system vgrG gene in the virulence and antimicrobial resistance of Acinetobacter baumannii ATCC 19606

Wang

Zhou

et al. 2018

PLoS ONE

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The Type VI Secretion System (T6SS) is an important virulence system that exists in many bacterial pathogens, and has emerged as a potent mediator of pathogenicity in Acinetobacter baumannii. In this study, we inactivated one of the T6SS components vgrG (valine–glycine repeat G) gene in A. baumannii ATCC 19606 and constructed a complementation strain. BEAS-2b human alveolar epithelial cells was adopted to assess bacterial adhesion, and wild female BALB/c mice were used for in vivo experiments to assess the bacterial killing ability to host. Upon deletion of the vgrG gene, increased antimicrobial resistance to ampicillin/sulbactam, but reduced resistance to chloramphenicol were observed. The vgrG mutant strain showed lower growth rate, reduced eukaryotic cell adherence and impaired lethality in mice. However, the vgrG mutant strain is not implicated in biofilm formation. Our study suggests that the Type VI Secretion System core component VgrG contributes to both virulence and antimicrobial resistance in A. baumannii ATCC 19606.

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Multiple roles for a novel RND‐type efflux system in Acinetobacter baumannii AB5075

Cited by 32 publications

References 54 publications

Roles of Efflux Pumps from Different Superfamilies in the Surface-Associated Motility and Virulence of Acinetobacter baumannii ATCC 17978

Roles of Efflux Pumps from Different Superfamilies in the Surface-Associated Motility and Virulence of Acinetobacter baumannii ATCC 17978

Genomic and Metabolic Insights into Denitrification, Sulfur Oxidation, and Multidrug Efflux Pump Mechanisms in the Bacterium Rhodoferax sediminis sp. nov.

The role of the type VI secretion system vgrG gene in the virulence and antimicrobial resistance of Acinetobacter baumannii ATCC 19606

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